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추출용매에 따른 어성초 추출물의 항산화 및 항노화 활성

Antioxidant and Anti-aging Activity of Houttuyniae Herba Extracts Obtained from Different Solvents

이양숙|김남우|서수정|장유수
대한미용학회 (The Korean Society for Investigative Cosmetology)|2019.06|대한미용학회지 (Journal of Investigative Cosmetology)|제15권 제2호|pp. 147-157|DOI 10.15810/jic.2019.15.2.004

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Antioxidant1Anti-agingHouttuyniae HerbaExtract solvents3-butylene glycol

초록

This study aimed to investigate antioxidant, anti-aging and cytotoxicities of Houttuyniae herba extracts in different solvents, viz. WE; water extract, EE; 70% ethanol extract, BGE; 1,3-butylene glycol. The contents of total polyphenol and flavonoid compounds of EE were 3,083.33 μg/mL and 728.79 μg/mL, respectively. The ABTS radical scavenging activity at 0.5% concentration in EE was 67.62%. The DPPH radical scavenging effect and SOD-like activity showed the highest effect of 74.65% and 43.83%, respectively in BGE at 3.0% concentration. Furthermore, the xanthine oxidase inhibition activity of BGE at 3.0% concentration in EE was 38.26%. Tyrosinase inhibition activity for whitening effect showed the highest value of 20.93% in WE. In the analysis of anti-wrinkle effect, the highest collagenase and elastase inhibition activities, viz. 53.70% and 47.51%, respectively were observed in EE. In the cytotoxicity test of the extracts on RAW264.7 macrophages and HaCaT keratinocytes using the MTT assay, RAW264.7 macrophages viability in WE decreased by about 30%, and decreased by about 22% in both EE and BGE. The HaCaT cell viability of WE decreased at the concentration of over 50 μg/mL. However, as the concentration of EE and BGE increased, the viability of RAW264.7 macrophage also increased. These results indicated that the ethanol extracts (EE) of Houttuyniae herba have useful ingredients, an excellent antioxidant and anti-aging activity. In addition to the water and ethanol, 1,3-butylene glycol can be used as a solvent for functional cosmetics materials extraction.

Abstract

This study aimed to investigate antioxidant, anti-aging and cytotoxicities of Houttuyniae herba extracts in different solvents, viz. WE; water extract, EE; 70% ethanol extract, BGE; 1,3-butylene glycol. The contents of total polyphenol and flavonoid compounds of EE were 3,083.33 μg/mL and 728.79 μg/mL, respectively. The ABTS radical scavenging activity at 0.5% concentration in EE was 67.62%. The DPPH radical scavenging effect and SOD-like activity showed the highest effect of 74.65% and 43.83%, respectively in BGE at 3.0% concentration. Furthermore, the xanthine oxidase inhibition activity of BGE at 3.0% concentration in EE was 38.26%. Tyrosinase inhibition activity for whitening effect showed the highest value of 20.93% in WE. In the analysis of anti-wrinkle effect, the highest collagenase and elastase inhibition activities, viz. 53.70% and 47.51%, respectively were observed in EE. In the cytotoxicity test of the extracts on RAW264.7 macrophages and HaCaT keratinocytes using the MTT assay, RAW264.7 macrophages viability in WE decreased by about 30%, and decreased by about 22% in both EE and BGE. The HaCaT cell viability of WE decreased at the concentration of over 50 μg/mL. However, as the concentration of EE and BGE increased, the viability of RAW264.7 macrophage also increased. These results indicated that the ethanol extracts (EE) of Houttuyniae herba have useful ingredients, an excellent antioxidant and anti-aging activity. In addition to the water and ethanol, 1,3-butylene glycol can be used as a solvent for functional cosmetics materials extraction.

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