魚腥草 추출물의 항여드름 효과에 관한 연구
The Effects of Houttuyniae Herba extract on the Activity of Anti-bacteria, Anti-inflammation and Anti-oxidation
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Objective : This experimental study was performed to investigate the effects of Houttuyniae Herba extract on anti-inflammation and anti-oxidation. Methods : The cytotoxicity of Houttuyniae Herba water extract and ethanol extract about viability of Raw 264.7 cell were tested using a colormetric tetrazolium assay(MTT assay). We investigated the inhibitory effects of Houttuyniae Herba water extract and ethanol extract on Propionibactrium acnes using paper disk diffusion method. To investigate the anti-inflammation effects of Houttuyniae Herba water extract and ethanol extract on LPS-induced macrophage Raw 264.7 cell, we used ELISA kit. We evaluated anti-oxidation effects of Houttuyniae Herba water extract and ethanol extract on HaCaT cell by Enzyme recycling method. Results : 1. In Houttuyniae Herba water extract and ethanol extract, cell toxicity depended on the density and wasn't difference between two extracts. 2. Houttuyniae Herba water extract and ethanol extract has not the significant inhibition effect of Propionibactrium acnes. 3. Concentration of 50, 100 ㎍/㎖ Houttuyniae Herba water extract inhibited the production of NO in the Raw 264.7 cell stimulated with LPS. 4. All extracts except for 20 ㎍/㎖ Houttuyniae Herba water extract showed anti-oxidation effect by decreasing the DPPH radicals. Conclusions : These results indicate that Houttuyniae Herba extract has anti-inflammation and anti-oxidation effects. If further study is performed, the use of Houttuyniae Herba extract will be valuable and benificial in the therapy of Propionibactrium acnes.
Abstract
Objective : This experimental study was performed to investigate the effects of Houttuyniae Herba extract on anti-inflammation and anti-oxidation. Methods : The cytotoxicity of Houttuyniae Herba water extract and ethanol extract about viability of Raw 264.7 cell were tested using a colormetric tetrazolium assay(MTT assay). We investigated the inhibitory effects of Houttuyniae Herba water extract and ethanol extract on Propionibactrium acnes using paper disk diffusion method. To investigate the anti-inflammation effects of Houttuyniae Herba water extract and ethanol extract on LPS-induced macrophage Raw 264.7 cell, we used ELISA kit. We evaluated anti-oxidation effects of Houttuyniae Herba water extract and ethanol extract on HaCaT cell by Enzyme recycling method. Results : 1. In Houttuyniae Herba water extract and ethanol extract, cell toxicity depended on the density and wasn't difference between two extracts. 2. Houttuyniae Herba water extract and ethanol extract has not the significant inhibition effect of Propionibactrium acnes. 3. Concentration of 50, 100 ㎍/㎖ Houttuyniae Herba water extract inhibited the production of NO in the Raw 264.7 cell stimulated with LPS. 4. All extracts except for 20 ㎍/㎖ Houttuyniae Herba water extract showed anti-oxidation effect by decreasing the DPPH radicals. Conclusions : These results indicate that Houttuyniae Herba extract has anti-inflammation and anti-oxidation effects. If further study is performed, the use of Houttuyniae Herba extract will be valuable and benificial in the therapy of Propionibactrium acnes.
참고문헌 (24)
- 안성구, "여드름 바이블", pp.62~ (2006), 진솔
- 강경희, "피부과학", pp.127~130 (2002), 청구문화사
- 황규왕, "여드름의 치료", 대한의사협회지, 49(1), pp.63~64 (2006)
- 의과대학 교수편, "임상약리학", pp.1138~1139 (1998), 한우리
- 전국한의과대학 본초학교실, "본초학", pp.212~213 (1991), 영림사
- 서부일, "알기쉬운 본초학", pp.97~98 (2004), 대구한의대학교출판부
- 안덕균, "한국본초도감", pp.98~ (2000), 교학사
- Nam-Kyoung Kim, "Studies on the antioxidative effect of the BuckWheat (Fagopyrum esculentum Moench) extract and its Protective Role against Cadmium-mediated stress", J. Soc. Cosmet. Scientists Korea, 31(2), pp.197~206 (2005)
- H. Kim, "Studies on the anti-acne effect of Agrimonia pilosa Ledeb", J. Soc. Cosmet. Scientists Korea, 32(1), pp.53~58 (2006)
- S. Lee, "Trichophyton and Pityrosporum, Antimicrobial effect of Bamboo (Phyllosrachys Bambusoides) Essential Oil on", J. Fd Hyg. Safety, 18(3), pp.113~117 (2003)
- T. Mosmann, "Rapid colorimetric assay for cellular growth and survival application of proliferation and cytotoxicity", J. Immunol. Methods, 65, pp.55~ (1983)
- Sang Eun Lee, "J. Screening of Traditional Herbral Medicines to Develop New Materials for Anti-aging and Anti-wrinkle in the skin", Soc. Cosmet. Scientists Korea, 31(2), pp.147~152 (2005)
- S. M. Han, "Anti-inflammatory effect of the venom from asian honeybees(Apis cerana L.) on inducible nitric oxide synthase and tumor necrosis factor-α in Raw 264.7 cell line", Korean J. Apiculture, 19, pp.89~ (2004)
- M. S. Blois, "Antioxidant determinanations by the use of a stable free radical", Nature, 181, pp.1199~ (1958)
- 李经纬, "중의대사전", pp.1285~ (1995), 인민위생출판사
- 陳實功, "外科正宗", pp.281~ (2000), 천진과학기술출판사
- 頋伯华, "실용중의외과학", pp.535~536 (1985), 상해과학기술출판사
- 오성문, "약용식물로부터 여드름 원인 미생물에 대한 천연 항균제 탐색", 석사, pp.2006
- 안성구, "여드름 바이블", pp.263~347 (2006), 진솔
- 신민교, "임상본초학", pp.574~578 (2000), 영림사
- 안은경, "천마 에탄올 추출물에 대한 항염증 활성", 석사, 약학과, pp.2006
- 하대식, "전통 약용식물의 항산화효과와 유전 독성에 관한 연구", 박사, pp.2006
- 서영국, "어성초 즙 발효제품의 항산화 및 항알레르기 활성", 석사, pp.2005
- 두인선, "5% 어성초 추출물 수용액의 외용요법이 여드름에 미치는 임상적 연구", 석사, pp.2004