괭이밥 초음파 추출물의 항노화 및 항산화 활성 비교
Comparison of Anti-aging and Antioxidant Activities of Oxalis corniculata Ultrasonic Extracts
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본 연구는 민간 및 약용식물로 사용되고 있는 괭이밥으로부 터 천연유래 기능성 화장료 소재로서의 가능성을 확인하기 위 하여 초음파 추출방법을 이용하여 물(UW)과 70% 에탄올(UE) 을 용매로 추출하여 각 추출물에 대한 항주름, 항산화 활성 및 세포독성과 NO 생성 저해 효과를 실험하였다. 총 폴리페놀은 UW(33.95 mg/g)가 UE(18.61 mg/g)보다 많았으나 총 플라 보노이드는 UE(20.28 mg/g)가 UW(10.26 mg/g)보다 약 2배 많이 함유하였다. Tyrosinase와 collagenase 저해 활성 그리고 ABTS 및 DPPH radical 소거율은 초음파 에탄올 추출물인 UE 가 UW보다 약 1.2배 이상 높은 효과를 나타내었으며, XOase 저해율도 UE(32.71%)가 UW(26.41%)보다 높은 효과를 나타 내었다. Elastase 저해 활성은 UW(48.00%)가 UE(41.75%)보 다 약간 더 높았으며, SOD 유사활성은 두 추출물간에 유의적 차이는 없었다. 아질산염 소거율은 pH 1.2의 조건에서는 UW 가 51.05%로 UE(46.60%)보다 높았으나 pH 3.0에서는 UW18.77%, UE 23.51%로 UW보다 약간 더 높은 아질산염 소거 효과를 나타내었다. MTT assay를 이용한 RAW264.7 대식세포 에 대한 세포독성을 측정한 결과 물 추출물인 UW가 10 μg/mL 에서 RAW264.7 대식세포가 대조군에 비하여 7.22%의 세포 생 존율이 감소하였으나 UW와 UE 모두 농도가 증가함에 따라 세 포생존율이 유의적으로 증가하였으며, RAW264.7 대식세포에 독성이 없는 것으로 나타났다. 그리고 RAW264.7 대식세포에 LPS를 처리한 후 생성되는 NO의 생성 억제를 분석한 결과, 괭 이밥 초음파 추출물에 의해 NO가 10% 정도 감소됨을 확인하였 다. 이러한 결과로 괭이밥 초음파 추출물에 대한 미백, 항주름 및 항산화 효과를 확인하였으며, 물보다는 에탄올을 용매로 추 출하는 것이 화장료 소재로 활용 가치가 좀 더 높은 것으로 판단 된다.
Abstract
Oxalis corniculata L. (Oxalidaceae) has been used as a natural remedy to help relieve hemorrhoids, boils and dermatopathies. This study was conducted to investigate the anti-aging, anti-oxidation activities and cell toxicity of water and ethanol extracts obtained from O. corniculata by ultrasonic extractor at 40℃, to characterize their practical utility. The content of total polyphenol compounds was higher UW (33.95 mg/g) than UE (18.61 mg/g), total flavonoid compounds was roughly twice as much UE (20.28 mg/g) than UW (10.26 mg/g). The tyrosinase inhibitory, collagenase inhibitory, ABTS and DPPH radical scavenging activities of the ultrasonic ethanol extract, UE were over 1.2 times higher than that of the UW. The xanthine oxidase inhibitory rate of UE (32.71%) was higher than UW (26.41%), in 1.0 mg/mL. The elastase inhibitory activity of UE and UW were 41.75% and 48.00%, respectively. Nitrite scavenging activity was the highest as 51.05% at UW at pH 1.2 at a concentration of 1.0 mg/mL. Cell viability, as determined using the MTT assay, was significantly increased with increasing concentrations of both UW and UE, and showed no toxicity to RAW264.7 macrophages. The inhibitory effect on NO production in LPS-stimulated RAW264.7 cells was decreased in about 10% of UW and UE. These results suggests that the ultrasonic water and ethanol extracts from O. corniculata have anti-aging and anti-oxidative properties and could be used as a natural source for the development of functional cosmetics.
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