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황련의 1,3-Butylene glycol과 혼합 추출물의 항주름 및 항산화 활성

Anti-wrinkle and Antioxidant Activity of 1,3-Butylene glycol Mixed Extracts from Coptidis Rhizoma

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초록

We evaluated the cytotoxicities and anti-wrinkle, antioxidant and whitening capacities of 1,3-butylene glycol and mixed solvent from Coptidis Rhizoma for potential use in cosmetic materials. The collagenase inhibition rates (ie, anti-wrinkle effects) were 81.95% (BGE; 1,3-butylene glycol extract from Coptidis Rhizoma) and 81.28% (WBG; water in 1,3-butylene glycol extract from Coptidis Rhizoma) at 3.0%. The elastase inhibition potential of BGE was higher than that of ascorbic acid (81.96% vs 68.06%, respectively). The tyrosinase inhibition rate of BGE was 11.20%. The DPPH radical scavenging potentials of BGE and EtBG (ethanol in 1,3-butylene glycol extract from Coptidis Rhizoma) were 86.04% and 85.37%, respectively. As for ABTs [2,2′-azinobis-(3-ethylbenzothiazoline-6- sulfonic acid)] radical scavenging and SOD-like activities, WBG showed the highest values-98.98% and 57.03% at a concentration of 3.0%, respectively. Xanthine oxidase inhibitory activities of BGE and EtBG were 94.64% and 94.18%, respectively. Measuring cytotoxicity using the MTT assay, RAW264.7 macrophage viability was increased at 25 ug/mL, however, as the concentration of extracts increased, the viability of RAW264.7 macrophages decreased. HaCaT cell viabilities decreased following treatment with WBG and EtBG at 25 ug/mL, but HaCaT cell viabilities increased at concentrations over 50 ug/mL. Our results revealed that the 1,3-BG and water and ethanol mixed extracts displayed anti-wrinkle and antioxidant activities. These extracts could be used as a functional anti-aging cosmetic material.

Abstract

We evaluated the cytotoxicities and anti-wrinkle, antioxidant and whitening capacities of 1,3-butylene glycol and mixed solvent from Coptidis Rhizoma for potential use in cosmetic materials. The collagenase inhibition rates (ie, anti-wrinkle effects) were 81.95% (BGE; 1,3-butylene glycol extract from Coptidis Rhizoma) and 81.28% (WBG; water in 1,3-butylene glycol extract from Coptidis Rhizoma) at 3.0%. The elastase inhibition potential of BGE was higher than that of ascorbic acid (81.96% vs 68.06%, respectively). The tyrosinase inhibition rate of BGE was 11.20%. The DPPH radical scavenging potentials of BGE and EtBG (ethanol in 1,3-butylene glycol extract from Coptidis Rhizoma) were 86.04% and 85.37%, respectively. As for ABTs [2,2′-azinobis-(3-ethylbenzothiazoline-6- sulfonic acid)] radical scavenging and SOD-like activities, WBG showed the highest values-98.98% and 57.03% at a concentration of 3.0%, respectively. Xanthine oxidase inhibitory activities of BGE and EtBG were 94.64% and 94.18%, respectively. Measuring cytotoxicity using the MTT assay, RAW264.7 macrophage viability was increased at 25 ug/mL, however, as the concentration of extracts increased, the viability of RAW264.7 macrophages decreased. HaCaT cell viabilities decreased following treatment with WBG and EtBG at 25 ug/mL, but HaCaT cell viabilities increased at concentrations over 50 ug/mL. Our results revealed that the 1,3-BG and water and ethanol mixed extracts displayed anti-wrinkle and antioxidant activities. These extracts could be used as a functional anti-aging cosmetic material.

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