금은화, 백선피, 연교 추출물의 항노화, 항산화 및 항염증 활성
Anti-aging, Antioxidant and Anti-inflammation Activities of Lonicerae Flos, Dictamni Radicis Cortex and Forsythiae Fructus Extracts
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초록
The current study intended to investigate anti-aging, antioxidant, cytotoxicity and anti-inflammatory activities of three herbal water extracts of Lonicerae Flos(LF), Dictamni Radicis Cortex(DRC) and Forsythiae Fructus(FF). The collagenase and elastase inhibition abilities of FF extract were 33.64% and 40.58% at 1,000 μg/mL, and xanthine oxidase inhibition rate was 23.05%. The tyrosinase inhibition rate of the three extracts were 25.12%~27.78% and showed similar inhibitory effects. DPPH and ABTS radical scavenging abilities of LF were 91.19% and 78.54%, respectively. The LF extract showed the highest value of nitrite scavenging ability of 91.30% at pH 1.2. In the cytotoxicity test of the extracts on RAW264.7 macrophages and HaCaT keratinocytes using the MTT assay, RAW264.7 macrophages viability of LF was decreased by about 20% at 20 μg/mL, whereas for FF the viability increased by 10% at 100 μg/mL. The HaCaT cell viabilities decreased by about 37% at 100 μg/mL for LF. The xanthine oxidase inhibition activity of DRC extracts was the highest, 62.21% at 1,000 μg/mL. The superoxide dismutase (SOD)-like activity was the highest, 23.05% for the FF extract. The inhibition of NO production of DRC decreased by about 47% in the treatment of 100 μg/mL, and decreased by about 43% and 35% for LF and FF, respectively. These results indicated that the LF and FF have useful ingredients, excellent anti-aging, antioxidant activity and nitrite scavenging ability. Therefore, it can be concluded that LF and FF water extract have the potential to be used as a functional ingredient for skin health and beauty food.
Abstract
The current study intended to investigate anti-aging, antioxidant, cytotoxicity and anti-inflammatory activities of three herbal water extracts of Lonicerae Flos(LF), Dictamni Radicis Cortex(DRC) and Forsythiae Fructus(FF). The collagenase and elastase inhibition abilities of FF extract were 33.64% and 40.58% at 1,000 μg/mL, and xanthine oxidase inhibition rate was 23.05%. The tyrosinase inhibition rate of the three extracts were 25.12%~27.78% and showed similar inhibitory effects. DPPH and ABTS radical scavenging abilities of LF were 91.19% and 78.54%, respectively. The LF extract showed the highest value of nitrite scavenging ability of 91.30% at pH 1.2. In the cytotoxicity test of the extracts on RAW264.7 macrophages and HaCaT keratinocytes using the MTT assay, RAW264.7 macrophages viability of LF was decreased by about 20% at 20 μg/mL, whereas for FF the viability increased by 10% at 100 μg/mL. The HaCaT cell viabilities decreased by about 37% at 100 μg/mL for LF. The xanthine oxidase inhibition activity of DRC extracts was the highest, 62.21% at 1,000 μg/mL. The superoxide dismutase (SOD)-like activity was the highest, 23.05% for the FF extract. The inhibition of NO production of DRC decreased by about 47% in the treatment of 100 μg/mL, and decreased by about 43% and 35% for LF and FF, respectively. These results indicated that the LF and FF have useful ingredients, excellent anti-aging, antioxidant activity and nitrite scavenging ability. Therefore, it can be concluded that LF and FF water extract have the potential to be used as a functional ingredient for skin health and beauty food.
참고문헌 (48)
- AOAC, "Official method of analysis", Method 965.31. Association of official analytical chemists, 19~ (2005)
- 배지현, "식중독 유발세균의 증식에 미치는 금은화 추출물의 항균효과", 한국식품과학회지, 37(4), pp.642~647 (2005), 한국식품과학회
- Bartsch H, "Inhibition of endogenous nitrosation: Mechanism and implications in human cancer prevention", Mutatio Res, 202, pp.307~324 (1988)
- Bensky D, "Chinese herbal medicine: Materia media", pp.85~86 (1986), Eastland press
- Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Cannell RJP, "Results of a large scale screen of microalgae for the production of protease inhibitors", Planta Med, 54, pp.10~14 (1988)
- 곡정강, "連翹의 LPS로 유도된 Raw 264.7 Cell에서의 抗炎症 효과", 대한한방내과학회지, 31(2), pp.242~253 (2010), 대한한방내과학회
- Cooney RV, "N-nitrosation and N-nitration of morphpline by nitrogen dioxide in aqueous solution: Effect of vanillin and related phenols", J Agric Food Chem, 35, pp.789~793 (1987)
- Crosby NT, "N-nitrosamines: A review of chemical and biological properties and their estimation in foodstuffs", Adv Food Res, 22, pp.1~71 (1976)
- del Marmol V, "Tyrosinase and related proteins mammalian pigmentation", FEBS Lett, 381, pp.165~168 (1996)
- Fellegrini N, "Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation de-colorization assay", Method Enzymol, 299, pp.379~389 (1999)
- Giacomoni PU, "Factors of skin ageing share common mechanism", Biogerontology, 2, pp.219~229 (2001)
- Greenstock CL, "Radiation and aging: free radical damage, biological response and possible antioxidant intervention", Med Hypotheses, 41, pp.473~482 (1993)
- Hatano T, "Inhibitory effects of galloylated flavonoids on xanthine oxidase", Planta Med, 57, pp.83~84 (1991)
- Huang KC, "The pharmacology of Chinese herbs", pp.8~9 (1998), CRS Press
- Jiang S, "Effects of a Dictamnus dasycarpus T. extract on allergic models in mice", Biosci Biotechnol Biochem, 72, pp.660~665 (2008)
- 주정석, "소회향과 금은화 추출물의 급성췌장염에 대한 예방효과", 대한본초학회지, 25(4), pp.39~45 (2010), 대한본초학회
- Jung SW, "Screening of tyrosinase inhibitor from plants", Korean J Food Sci Technol, 27, pp.891~896 (1995)
- Kato H, "Inhibition of nitrosamine formation by nondialyzable melanoidins", Agric Biol Chem, 51, pp.1333~1338 (1987)
- Kim JH, "Fraxinellone inhibits lipopolysaccharide-induced inducible nitric oxide synthase and cyclooxygenase-2 expression by negatively regulating nuclear factor-kappa B in RAW 264.7 Anti-aging, Antioxidant and Anti-inflammation Activities of Three Herbal Extractsmacrophages cells", Biol Pharm Bull, 32, pp.1062~1068 (2009)
- 김수화, "피부의 노화와 주름 억제, 미백효과를 가지는 전통 한약재의 탐색", 동의생리병리학회지, 22(3), pp.691~698 (2008), 한의병리학회
- Kim TJ, "Korean resources plant", pp.262~ (1991), Seoul National University Publish
- Lee JM, "Use of oriental herbs as medical food", Food Indust Nutr, 5, pp.50~56 (2000)
- 임정대, "한국산 약용식물로부터의 SOD 활성 및 Phenolic Compounds 함량 비교", 한국약용작물학회지, 12(3), pp.191~202 (2004), 한국약용작물학회
- Luo ZH, "The combined modulating effects of cerium nitrate with certain Chinese traditional drugs on altered cell-mediated immunities in scald mice", Zonghua wai Ke Za Zhi, 28, pp.562~565 (1990)
- Marklund S, "Involvement of superoxide amino radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase", Eur J Biochem, 47, pp.468~474 (1975)
- 나경민, "생약재 추출물의 생리활성 효과", Food Science and Preservation, 11(3), pp.388~393 (2004), 한국식품저장유통학회
- Nieva Moreno MI, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina", J Ethnopharmacol, 71, pp.109~114 (2000)
- Nishibe S, "Studies on the Chinese crude drug "Forsythiae Fructus." VI. The structure and antibacterial activity of suspensaside isolated from Forsythia suspensa", Chem Pharm Bull, 30, pp.4548~4553 (1982)
- Peter FS, "The toxicology of nitrate, nitrite and n-nitroso compounds", J Sci Food Agric, 52, pp.1761~1764 (1975)
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic Biol Med, 26, pp.1231~1237 (1999)
- Rim BM, "Effects of Lonicera japonica extracts as a biological response modifier", Environ Mut Car, 12, pp.45~54 (1992)
- Rittié L, "UV-light-induced signal cascades and skin aging", Ageing Res Rev, 1, pp.705~720 (2002)
- Seo SY, "Mushroom tyrosinase: Recent prospects", J Agric Food Chem, 51, pp.2837~2853 (2003)
- Shin DH, "Antimicrobial effect of ethanol extracts from some medicinal herbs and their fractionates against food-born bacteria", Korean J Food Sci Technol, 29, pp.808~816 (1997)
- 신유진, "한약재 열수추출물의 항산화 활성 및 Xanthine Oxidase 저해 활성", 한국식품영양과학회지, 42(10), pp.1712~1716 (2013), 한국식품영양과학회
- 송선영, "금은화의 폴리페놀, 플라보노이드, Chlorogenic acid의 최적 추출조건", 공학기술논문지, 11(1), pp.1~5 (2018), 공학기술연구원[DOI]
- Stirpe F, "The regulation of rat liver xanthine oxidase", J Biol Chem, 244, pp.3855~3863 (1969)
- Surveswaran S, "Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants", Food Chem, 102, pp.938~953 (2007)
- Talwar HS, "Reduced type I and type III procollagens in photodamaged adult human skin", J Invest Dermatol, 105, pp.285~290 (1995)
- Tsukahara K, "Inhibition of ultraviolet-B-induced wrinkle formation by an elastase-inhibiting herbal extract: Implication for the mechanism underlying elastase-associated wrinkles", Int J Dermatol, 45, pp.460~468 (2006)
- Wünsch E, "Zur quantitativen bestimmung der kollagenase", Hoppe-seyler’s Zeitschrift Fur Physiologische Chemie, 333, pp.149~151 (1963)
- Wyngaarden JB, "Molecular nature of enzyme regulation in purine biosynthesis", Ciba Found Symp, 48, pp.43~64 (1977)
- Yagi A, "Inhibition of mushroom-tyrosinase by aloe extract", Planta Med, 53, pp.515~517 (1987)
- 양서진, "연교 추출물의 항산화활성 및 미백 효과", 한국약용작물학회지, 19(6), pp.472~477 (2011), 한국약용작물학회
- Yoon JS, "Limonoids from Dictamnus dasycarpus protect against glutamate-induced toxicity in primary cultured rat cortical cells", J Mol Neurosci, 42, pp.9~16 (2010)
- 윤경진, "금은화 추출액이 RAW 264.7 Macrophage에서의 NO와 PGE2 생성에 미치는 영향", Journal of Acupuncture Research, 29(1), pp.67~74 (2012), 대한침구의학회
- Zhao W, "Antifungal alkaloids and limonoid derivatives from Dictamnus dasycarpus", Phytochemistry, 47, pp.7~11 (1998)