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화장료 소재개발을 위한 5종 약용식물 열수추출 혼합물의 항산화성 연구

Antioxidant Activity of Water Extracts from 5 Medicinal Herbs Mixture for Cosmetic Material Development

장민정|이양숙|서수정
대한미용학회 (The Korean Society for Investigative Cosmetology)|2021.09|대한미용학회지 (Journal of Investigative Cosmetology)|제17권 제3호|pp. 275-283|DOI 10.15810/jic.2021.17.3.007

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키워드

AntioxidantC. chinensisH. cordataMedicinal herbs mixtureS. glabrae

초록

The present study aimed to examine the antioxidant activity and cytotoxities of cosmetic material development mixture extracts (C. chinensis, P. amurense, H. cordata, S. glabrae and L. bicolor) with different extraction mixing ratios (ME1, 1:1:1:1:1; ME2, 1:1:2:1:1; ME3, 1:1:4:1:2; ME4, 1:1:6:1:2). The results showed that superoxide dismutase (SOD)-like activity was the highest, and 19.24% for the ME4 at 1,000 ㎍/mL. In the measurement of the radical scavenging of ABTS and DPPH, ME3 showed the highest scavenging rates of 97.34% and 99.81%, respectively, at a concentration of 1,000 ㎍/mL. The collagenase and elastase inhibition abilities of ME3 and ME2 mixtures were found to be 47.09% and 17.19% at 1,000 ㎍/mL, and at concentration of 1,000 ㎍/mL, tyrosinase showed the highest inhibition activities 57.3% in ME3. Furthermore, cytotoxicity test of the extract mixture on RAW264.7 macrophages and HaCaT keratinocytes was conducted using the MTT assay. The RAW264.7 macrophages and HaCaT keratinocytes cell toxicity revealed that cell survival did not reduce at 25-100 ㎍/mL concentration. The inhibition of NO production of 5 medicinal herbs mixture decreased by about 13%-15% in the treatment of 100 ㎍ /mL. These results indicated that both ME3 and ME4 have excellent anti-aging and antioxidant activity. Therefore, it can be concluded that ME3 and ME4 with 5 medicinal herbs mixture have the potential to be used as functional ingredient for beauty food and skin health.

Abstract

The present study aimed to examine the antioxidant activity and cytotoxities of cosmetic material development mixture extracts (C. chinensis, P. amurense, H. cordata, S. glabrae and L. bicolor) with different extraction mixing ratios (ME1, 1:1:1:1:1; ME2, 1:1:2:1:1; ME3, 1:1:4:1:2; ME4, 1:1:6:1:2). The results showed that superoxide dismutase (SOD)-like activity was the highest, and 19.24% for the ME4 at 1,000 ㎍/mL. In the measurement of the radical scavenging of ABTS and DPPH, ME3 showed the highest scavenging rates of 97.34% and 99.81%, respectively, at a concentration of 1,000 ㎍/mL. The collagenase and elastase inhibition abilities of ME3 and ME2 mixtures were found to be 47.09% and 17.19% at 1,000 ㎍/mL, and at concentration of 1,000 ㎍/mL, tyrosinase showed the highest inhibition activities 57.3% in ME3. Furthermore, cytotoxicity test of the extract mixture on RAW264.7 macrophages and HaCaT keratinocytes was conducted using the MTT assay. The RAW264.7 macrophages and HaCaT keratinocytes cell toxicity revealed that cell survival did not reduce at 25-100 ㎍/mL concentration. The inhibition of NO production of 5 medicinal herbs mixture decreased by about 13%-15% in the treatment of 100 ㎍ /mL. These results indicated that both ME3 and ME4 have excellent anti-aging and antioxidant activity. Therefore, it can be concluded that ME3 and ME4 with 5 medicinal herbs mixture have the potential to be used as functional ingredient for beauty food and skin health.

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