지구성훈련 후 일회성 운동시 흰쥐 골격근 내 Small HSP 및 HSP 70과 Cytochrome c의 발현
Expression of αB-crystallin, HSP 27, HSP 70 and Cytochrome c in rat skeletal muscle following acute exercise between training and nontraining group
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본 연구의 목적은 지구성 훈련 후 일회성 운동시 흰쥐 골격근에서 small HSP, HSP 70과 cytochrome c가 어떠한 발현양상을 나타내는지 규명하는 것이다. 실험방법으로 SD 흰쥐를 저강도 지구성훈련집단(16m/min, 10°)과 고강도 지구성훈련집단(27m/min, 10°)로 나누어 8주간 지구성훈련을 실시하고 96시간의 운동중단을 시킨 후 일회성운동집단, 저강도/고강도 훈련집단에게 일회성운동(23m/min, 10°)를 실시하였다. 골격근은 가자미근과 장지신근을 적출하였으며 western 전기영동 분석방법을 이용하여 분석하고 유의수준은 p<.05수준으로 한 결과는 다음과 같다. 일회성 운동집단보다 지구성 훈련집단에서 세포사 및 근손상지표가 제한적으로 나타나 aB-crystallin과 HSP 27의 발현에 영향을 미친 것으로 나타났으며, HSP 70의 경우에는 훈련집단에서 회복기에 유의하게 증가하는 것으로 나타났다. 이러한 결과, small HSP는 운동으로 인한 손상 초기에 증가하여 회복기까지 지속되는 요인의 영향을 받아 증가하여 근골격과 IF(intermediate filament)의 항상성 유지를 위해 기능하며, HSP 70의 경우 주로 운동으로 인한 손상의 재생과 생합성에 관여하는 것으로 사료된다.
Abstract
This study was performed to analysis the expression of αB-crystallin, HSP 27, HSP 70 and cytochrome c by acute exercise in training and nontraining groups. The Male SD rat trained to run treadmill for 16M/min, 10° intensity(LT group) and 27M/min, 10° intensity(HT group) during 8 weeks. After interrupting 96H exercise, acute exercise and endurance training groups ran the treadmill running for 90min, 23m/min, 10°. Soleus/extensor digitorum longus muscle was extracted at the end of exercise and 24H recovery period. Small HSP, HSP 70, cytochrome c and muscle damage factor were analysed by western blotting. Small HSP were significantly increased in early stage of exercise. Also HSP 70 expression at the recovery. These results showed that the small HSPs may function as stabilizer of disrupted myofibrillar structure at the early stage of execise. and HSP 70 expression at the recovery period showed that the HSP 70 was involved in recovery and remodeling and adaptation processed in skeletal muscle.
참고문헌 (34)
- 김정규, "평지와 내리막 달리기 수행이 creatine kinase 활성도와 백혈구 및 혈중 HSP72 발현에 미치는 영향", 운동과학, 16(2), pp.115~124 (2007), 한국운동생리학회
- 박대령, "일회성 운동 및 회복기 흰쥐 골격근 내 Small HSP 및 HSP 70과 골격근 손상인자 발현 양상에 대한 연구", 운동과학, 18(3), pp.285~294 (2009), 한국운동생리학회
- 신영오, "장기간의 운동이 쥐의 심장근과 골격근의 HSP 72발현 변화에 미치는 영향", 한국체육학회지, 40(2), pp.655~662 (2001)
- Bedford, T. G, "Maximum oxygen consumption of rats and its changes with various experimental procedures", J. Appl. Physiol, 47(6), pp.1278~1283 (1979)
- Calderwood, S. K, "Extracellular heat shock protein in cell signaling", FEBS. Lett, 581, pp.3689~3694 (2007)
- Chen, Y, "Alternations in the expression and activity of creatine kinase-M and mitochondrial creatine kinase subunit in skeletal muscle following prolonged intense exercise in rats", Eur. J. Appl. Physiol, 81, pp.114~119 (2000)
- Folkesson, M, "Immunohistochemical changes in the expression of HSP 27 in exercised human vastus lateralis muscle", Acta. Physiol, 194, pp.215~222 (2008)
- Gabai, V. L., "Invited review : Interplay between molecular chaperon & signaling pathway in survival of heat shock", J. Appl. Physiol, 92, pp.1743~1748 (2002)
- Gorog. D. A, "MAPKAPK-2 modulates p38-MAPK localization and small heat shock protein phosphorylation and small heat shock protein phosphorylation but not mediate the injury associated with p38-MAPK activation during myocardial ischemia", Cell Stress and Chaperones, 14, pp.477~489 (2009)
- Hamilton, K. L, "Short-term exercise training can improve myocardial tolerance to I", R without elevation in heat shock proteins, 2001, pp.281, H1346~H1352
- Ji, L. L, "Acute exercise activates nuclear factor (NF)-KappaB signaling pathway in rat skeletal mucsle", FASEB. J, 18, pp.1499~1506 (2004)
- Koh, T. J, "Cytoskeletal disruption and small heat shock protein translocation immediately after lengthening contractions", Am. J. Physiol. Cell. Physiol, 286, pp.c713~c722 (2004)
- Komulainen, K, "The disruption of myofibre structure in rat skeletal muscle after forced lengthening contraction", Eur. J. Physiol, 436, pp.735~741 (1998)
- Kregel, K. C, "Molecular biology of thermoregulation Invite review : Heat shock protein : modifiying factors in physical stress responses and acquires thermotolerance", J. Appl. Physiol, 92, pp.2177~2186 (2002)
- Launay, N, "Cell signaling pathway to αB-crystallin following stresses of the cytoskeleton", Experimental cell research, 312, pp.3570~3584 (2006)
- Lethi, T. M, "Repeated bout effect on the cytoskeletal protein titin, desmin and dystrophin in rat skeletal muscle", J. Muscle. Res. Cell. Motil, 28(1), pp.39~47 (2007)
- Martindale, J. L, "Cellular response to oxidative stress; signaling for suicide and survival", J. Cell. Physiol, 192(1), pp.1~15 (2002)
- McArdle, A, "Exercise and skeletal muscle Aging : celluar and molecular mechanism", Ageing. Res. Rev, 1(1), pp.79~93 (2002)
- McCully, K. K, "Exercise-induced injury to skeletal muscle", Federation. Proc., 통권 : 45, pp.2933~2936 (1986)
- Melling. C. W, "Myocardial HSP 70 phosphorylation and PKC-mediated cardioprotection following exercise", Cell Stress and Chaperones, 14, pp.141~150 (2009)
- Milne, K. J, "Exercise-induced elevation of HSP70 is intensity dependent", J. Appl. Physiol, 93, pp.561~568 (2002)
- Morton, J. P, "Time course and differential response of major heat shock protein families in human skeletal muscle following acute nondamaging treadmill exercise", J. Appl. Physiol, 101, pp.176~182 (2006)
- Maglara, A. A, "Damage to developing mouse skeletal muscle myotubes in culture : protective effect of heat shock proteins", J Physiol, 543, pp.837~846 (2003)
- Noble, E. G, "Heat shock proteins and exercise : a primer", Appl. Physiol. Nutr. Metab, 33, pp.1050~1065 (2008)
- Noble, E, G, "Exercise is the primary factor associated with HSP70 induction in muscle of treadmill running rats", Acta. Physiol, 187, pp.495~501 (2006)
- Paulsen, P. G, "Maximal eccentric exercise induces a rapid accumulation of small heat shock proteins on myofibrils and a delayed HSP70 response in humans", Am. J. Physiol. Regul. Integr. Comp. Physiol, 293, pp.R844~R853 (2007)
- Rose, A. J, "Effect of exercise on protein kinase C activity and localization in human skeletal muscle", J. Physiol, 561(3), pp.861~870 (2004)
- Rőckl, K. S, "Signaling mechanism in skeletal muscle : acute response and chronic adaptation to exercise", IUBMB. Life, 60(3), pp.145~153 (2008)
- Sachdev, B, "Production, detection, and adaptive response to free radical in exercise", Free Radical Biology & medicine, 44, pp.215~223 (2008)
- Samelman, T. R, "Endurance training increases the expression of mitochondrial and nuclear encoded cytochrome c oxidase subunits and heat shock proteins in rat skeletal muscle", Eur. J. Appl. Physiol, 83(1), pp.22~27 (2000)
- Thompson, H. S, "Exercise-induced HSP27, HSP70 and MAPK response in human skeletal muscle", Acta. Physiol. Scand, 178, pp.61~72 (2003)
- Tiidus, P. M, "Skeletal muscle damage and repair", pp.5~6 (2008), Human Kinetic
- Voegeli, T. S, "Heat shock proteins 27 and 70 regulating angiotensin Ⅱ-induced NF-κB: a possible connection to blood pressure control?", Appl. Physiol. Nutr. Metab, 33(5), pp.1042~1049 (2008)
- Vissing, K. H, "Heat shock protein translocation and expression response is attenuated in response to repeated eccentric exercise", Acta. Physiol, 196(3), pp.283~293 (2008)