비만유도 노화 흰쥐의 복합 운동이 대동맥, 골격근 및 심근의HSP70 및 SOD1 발현에 미치는 영향
Effects of Combine Exercise on HSP70 and SOD1 Expression of Aorta, Skeletal Muscle and Myocardium in High Fat Diet induced Obese Aging Rats
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PURPOSE: Exercise improve myocardial cell protection and vascular function through cell repair and suppression of oxidative stress incardiovascular diseases caused by aging. This study aimed to investigate the effect of combine exercise on HSP70 and SOD1 proteinexpression of aorta, skeletal muscle and myocardium in high fat diet induced obese aging rats. METHODS: Male 50-week-old Sprague Dawley rats (n=40) were divided into normal diet (ND, n=10), normal diet+exercise (NDEx,n=10), high fat diet (HFD, n=10), and high fat diet+exercise (HFDEx, n=10) groups. After six weeks on a high fat diet to induce obesity,a 12-week combine exercise program was implemented, which combine exercise (treadmill running+ladder climbing) three times aweek for 45 minutes per session. RESULTS: Body weight was significantly decreased after 12 weeks combine exercise program compared to the ND group (p<.05) andHFDEx group compared to the HFD group (p<.05), respectively. After completing the 12-week exercise program, heat shock protein70 (HSP70) and superoxide dismutase 1 (SOD1) expressions were significantly (p<.05) higher in the NDEx group compared to theND group in the myocardium. Also, SOD1 protein expression was significantly (p<.05) higher in the NDEx group compared to the NDgroup and HFDEx group compared to the HFD group in the skeletal muscle. CONCLUSIONS: : In conclusion, combine exercise intervention of high fat diet-induced obesity resulted in decreased cell repair proteinand antioxidant enzyme protein in the myocardium. Therefore, it is thought that combine exercise intervention for obese inducedrats improved the cell repair protein and antioxidant enzyme activity of the myocardium.
Abstract
PURPOSE: Exercise improve myocardial cell protection and vascular function through cell repair and suppression of oxidative stress incardiovascular diseases caused by aging. This study aimed to investigate the effect of combine exercise on HSP70 and SOD1 proteinexpression of aorta, skeletal muscle and myocardium in high fat diet induced obese aging rats. METHODS: Male 50-week-old Sprague Dawley rats (n=40) were divided into normal diet (ND, n=10), normal diet+exercise (NDEx,n=10), high fat diet (HFD, n=10), and high fat diet+exercise (HFDEx, n=10) groups. After six weeks on a high fat diet to induce obesity,a 12-week combine exercise program was implemented, which combine exercise (treadmill running+ladder climbing) three times aweek for 45 minutes per session. RESULTS: Body weight was significantly decreased after 12 weeks combine exercise program compared to the ND group (p<.05) andHFDEx group compared to the HFD group (p<.05), respectively. After completing the 12-week exercise program, heat shock protein70 (HSP70) and superoxide dismutase 1 (SOD1) expressions were significantly (p<.05) higher in the NDEx group compared to theND group in the myocardium. Also, SOD1 protein expression was significantly (p<.05) higher in the NDEx group compared to the NDgroup and HFDEx group compared to the HFD group in the skeletal muscle. CONCLUSIONS: : In conclusion, combine exercise intervention of high fat diet-induced obesity resulted in decreased cell repair proteinand antioxidant enzyme protein in the myocardium. Therefore, it is thought that combine exercise intervention for obese inducedrats improved the cell repair protein and antioxidant enzyme activity of the myocardium.
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