트레드밀 운동이 추위 스트레스에 의한 생쥐 해마의 신경세포 사멸에 미치는 영향
Effect of treadmill exercise on cold stress-induced apoptotic neuronal cell death in the hippocampus in mice
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초록
본 연구는 추위 노출로 인한 스트레스에 의해 유발된 해마의 신경세포 사멸에 대한 트레드밀 운동의 효과를 규명하는데 그 목적이 있다. 본 실험을 위하여 체중 30-40g(8주령) ICR 계열 수컷 생쥐 총 20마리를 각각 통제집단(con, n=5), 트레드밀 운동집단(ex, n=5), 추위 스트레스 유발집단(cold, n=5), 추위 스트레스 유발 후 트레드밀 운동집단(cold+ex, n=5)의 4군으로 분류하였다. 추위 스트레스 유발집단과 추위 스트레스 유발 후 트레드밀 운동집단에게는 매일 3시간씩 주 6회, 3주간시간씩의 방에서 추위 스트레스에 노출시켰다. 트레드밀 운동집단과 추위 스트레스 유발 후 트레드밀 운동집단에게는 소형 동물용 트레드밀을 이용하여 추위 노출 직후 5 m/min의 속도로 1일 30분간의 달리기 운동을 주 6회, 3주간시실시하였다. 해마의 신경세포 사멸을 확인하기 위하여 caspase-3의 발현을 면역조직화학법을 이용하여 분석하였고, DNA의 분절을 확인하기 위하여 위염색법을 실시하였다. 본 실험 결과 추위 스트레스 유발은 caspase-3의 발현과 TUNEL 양성세포수를 유의하게 증가시켰고(p<.05), 추위 스트레스 유발 후 트레드밀 운동은 caspase-3의 발현과 TUNEL 양성세포수를 유의하게 감소시켰다(p<.05). 본 실험을 통하여 추위 스트레스에 의하여 유발될 수 있는 해마의 신경세포 사멸에 트레드밀 운동의 보호 작용이 있음을 알 수 있었다.
Abstract
In the present study, the effect of treadmill exercise on chronic intermittent cold stress-induced apoptotic neuronal cell death in the hippocampus of mice was investigated. Male ICR mice were divided into four groups: the control-rest group; the control-exercise group; the cold-rest group; and the cold-exercise group (n = 5 in each group). In the present study, the chronic intermittent cold stress was induced by exposure of mice to cold room at 4℃ for 3 h once a day, 6 times per week during 3 weeks. The treadmill exercise load consisted of running at a speed of 5 m/min for 30 min once a day, 6 times per week for 3 weeks. Immunohistochemistry for caspase-3 and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL) staining were performed to determine apoptotic neuronal cell death in the hippocampal dentate gyrus. Our results revealed that the expression of caspase-3 and the number of TUNEL-positive cells were significantly increased by cold stress (p<.05), and treadmill exercise significantly suppressed the expression of caspase-3 and the number of TUNEL-positive cells after cold stress (p<.05). The findings of our study provide evidence that treadmill exercise may ameliorate the brain damage induced by cold stress.
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