일회성 운동 및 회복기 흰쥐 골격근 내 Small HSP 및 HSP 70과 골격근 손상인자 발현 양상에 대한 연구
Expression of αB-crystallin, HSP 27, HSP 70 and muscle damage factors in rat skeletal muscle following acute endurance exercise and recovery
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초록
본 연구의 목적은 일회성 운동 및 회복기에 흰쥐 골격근에서 Small HSP군과 HSP 70 그리고 골격근 손상인자가 어떠한 발현양상을 나타내는지 규명하는 것이다. 실험방법으로는 SD 흰쥐가 26M/min, 10도의 강도에서 트레드밀 달리기를 실시하였으며, 운동시작 후 15분, 30분, 45분, 60분, 90분과 회복기 6H, 12H, 36H, 48H, 72H에 조직을 적출하였다. 분석을 위한 조직은 가자미근과 장지신근을 적출하였으며, Westren 전기영동 분석방법을 이용하여 분석을 실시하고 유의수준은 p<.05수준으로 한 결과는 다음과 같다. Small HSP인 αB-crystallin과 HSP 27은 운동 초기의 손상과 관련되어 유의하게 증가하는 것으로 나타났으며, 이러한 결과는 운동초기의 골격근 구조의 손상을 예방하고 안정화시키기 위해 small HSP군이 중요한 기능을 하는 것으로 사료된다. 또한, 회복기에 나타난 HSP 70의 지연된 발현은 HSP 70이 주로 손상에 따른 회복 및 재생과 관련되어 있음을 제시하는 것으로 사료된다.
Abstract
This study was to investigate to the expression of αB-crystallin, HSP 27, HSP 70 and muscle damage factor by acute endurance exercise and recovery. The Male SD rat ran the treadmill running for 90min 26M/min, 10° intensity and Soleus and extensor digitorum longus muscle was extracted at 15, 30, 45, 60, 90 min in exercise period and 6H, 12H, 24H, 36H, 48H in recovery period. Expression of αB-crystallin, HSP 27, HSP 70 and muscle damage factor were analyzed by western blotting. The expression of αB-crystallin, HSP 27 was significantly increased in early phase of exercise and HSP 70 was significantly increased in recovery period. The expression of cytosolic cytochrome c were significantly increased in recovery period. These results suggests that the small HSPs may function as stabilizer of disrupted myofibrillar structure at the early stage of execise. and Delayed HSP 70 expression at the recovery period showed that the HSP 70 was involved in recovery and remodeling and adaptation processed in skeletal muscle.
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