단기간과 장기간 지구성 운동에 따른 골격근 내 대사적응
Metabolic adaptation by acute and chronic endurance swim exercise in skeletal muscle.
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지구성트레이닝에 따른 해당효소의 역할은 미토콘드리아효소에 비해 명확하게 밝혀져 있지 않을 뿐 아니라, 미토콘드리아의 적응이 일부분만 이루어진 상황에서의 해당효소 작용 또한 규명된 것이 없다. 이에 본 연구는 지구성트레이닝에 따른 에너지대사 적응이 어떻게 이루어지는가를 알아보기 위해 단기간과 장기간 지구성트레이닝 후 미토콘드리아 전자전달 효소와 해당효소의 발현정도를 분석하였다. 이를 위해 24마리의 흰쥐를 각각 6마리씩 4그룹(sedentary; Sed, 1 day exercise; 1d Ex, 3 day exercise; 3d Ex, 3 weeks exercise; 3wk Ex)으로 분류한 다음 수영을 실시하였다. 미토콘드리아 전자전달 효소와 해당효소의 발현 분석을 위한 골격근은 마지막 운동훈련 18시간 후 적출하였다. 그 결과 해당효소인 총 LDH와 GAPDH는 Sed 그룹에 비해 1d Ex 그룹(60 %)과 3d Ex 그룹(80 %)은 유의하게 감소하였으며, 3d Ex 그룹은 1d Ex 그룹과도 유의한 차이를 보였다. 그러나 3wk Ex 그룹은 Sed 그룹과 비슷한 수준을 보였다. 그리고 HK와 LDH-H는 3d Ex 그룹과 3wk Ex 그룹이 Sed 그룹에 비해 유의하게 높게 나타났다. 미토콘드리아 효소 중 β-산화효소인 LCAD는 1d Ex, 3d Ex 그리고 3wk Ex 그룹 모두 Sed 그룹에 비해 유의하게 증가하였으며, 3d Ex 그룹은 1d Ex와 3wk Ex 그룹보다도 유의하게 높게 나타났다. 미토콘드리아 전자전달계 효소인 COX4와 NADH는 1d Ex, 3d Ex 그리고 3wk Ex 그룹 모두 Sed 그룹에 비해 유의하게 증가하였다. 또한 단기간 지구성트레이닝시 미토콘드리아 효소와 해당효소간에는 유의한 부적상관관계가 있으며, 장기간 지구성트레이닝시에는 아무런 상관관계가 없었다.
Abstract
The Effect of chronic and acute endurance training on the role of glycolytic enzymes compared to the mitochondrial enzymes has not been clearly identified. This study measured mitochondrial electron transport enzymes and glycolytic enzymes to evaluate the metabolic adaptation after acute and chronic endurance training. Twenty-four rats were randomly assigned into 4 groups(Sedentary, Sed; 1 day exercise, 1d Ex; 3 day exercise, 3d Ex; 3 weeks exercise, 3wk Ex). Exercise groups were trained by using a 6h/day swimming program. Muscle samples for mitochondrial enzymes and glycolytic enzymes were harvested 18 hours after the last bout of exercise. The expressions of total LDH and GAPDH in glycolytic enzymes were decreased in the 1d Ex(60 %) and 3d Ex(80 %) group from the Sed group. But, 3wk Ex group was not changed compared with Sed group. The expressions of HK and LDH-H form were increased in 3d Ex and 3wk Ex group compared to the Sed group. The expression of mitochondrial β-oxidation enzyme LCAD was increased more in the 1d Ex, 3d Ex and 3wk Ex than the Sed group, with the 3d Ex group having the most increase. The expressions of mitochondrial electron transport enzymes COX4 and NADH were increased in all exercise groups. There is a negative correlation between mitochondrial enzymes and glycolytic enzymes by acute endurance exercise, but by prolonged endurance exercise there is no correlation.
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