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일회성 유산소 운동에 따른 흰쥐 비복근 내 미토콘드리아 기능 및 구조 조절 단백질의 발현

The effect of acute exercise on the expression of mitochondria functiom and content protein in rat skeletal muscle

강윤석|김재철|김정하|박대령
한국운동생리학회|2012.05|운동과학 (Exercise Science)|제21권 제2호|pp. 191-200|DOI 10.15857/ksep.2012.21.2.191

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참고문헌

키워드

p53skeletal muscle골격근αB-crystallinPGC-1α일회성 유산소 운동Drp 1Mfn 2acute endurance exercise

초록

일회성 유산소 운동(26 m/min, 10°)에 따른 운동 중(45, 90 min)과 회복기(12 h)시 흰쥐 비복근 내 Drp 1, Mfn 2, PGC-1α, p53 및 αB-crystallin 단백질의 발현을 규명하는데 연구의 목적이 있으며, 골격근내 단백질 발현은 western blotting 방법을 통해 분석하였다. 미토콘드리아 기능 및 구조와 관련된 인자인 Drp 1, Mfn 2,PGC-1α의 발현이 적색 비복근에서 운동 45분과 회복기 12시간에서 높게 증가한 반면 백색 비복근에서는 증가하지 않는 것으로 나타났다. αB-crystallin은 적색 비복근에서 운동 45분에 증가하였고 p53의 경우 적색 비복근과 백색 비복근 모두 운동45분과 90분, 회복기 12시간에서 증가하는 것으로 나타났다. 이러한 결과는 Drp 1, Mfn 2, PGC-1α, p53, 및 αB-crystallin이운동 중 골격근내 미토콘드리아 기능과 구조의 변화에 적응을 유도하여 미토콘드리아의 항상성 유지에 긍정적으로 작용하고 있는 것으로 사료된다.

Abstract

The aim of this study was to investigate the expression of Drp 1, Mfn 2, PGC-1α, p53 and αB-crystallin protein in the skeletal muscle of Sprague-Dawley rats by endurance exercise(26 m/min, 10°,45 min, 90 min, recovery 12 h). The expression of Drp 1, Mfn 2, PGC-1α, p53 and αB-crystallin protein in skeletal muscle analyzed by western blotting. The expression of Drp 1, Mfn 2 and PGC-1α protein significantly were increased in red gastrocnemius during exercise(45 min) and recovery 12 h. The expression of B-crystallin protein significantly were increased in red gastrocnemius during exercise(45 min). The expression of p53 protein in both red and white gastrocnemius was increased in all periods. These findings suggest that Drp 1, Mfn 2, PGC-1α, p53 and αB-crystallin protein plays a role of mitochondrial homeostasis in skeletal musce by endurance exercise.

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