대복피 추출물의 분획별 항균 및 항산화 활성
Anti-bacterial and Anti-oxidant Activities of Solvent Fractions of Arecae pericarpium Extracts
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목적: 본 연구는 대복피 메탄올(methanol, MeOH) 추출물 및 용매 분획물의 항산화 및 항균활성을 확인하고자 수행되었다. 방법: 대복피를 80% MeOH로 추출하고, n-hexane, ethyl acetate (EtOAc), n-butanol, H2O로 분획하였다. 추출물과 분획물의추출 수율, 총 폴리페놀 함량을 측정하였다. 대복피의 80% MeOH 추출물과 용매 분획물의 free radical 소거능은 1,1-diphenyl- 2-picrylhydrazyl (DPPH) 방법에 의해 평가하였다. 항산화 활성은 superoxide dismutase (SOD) 및 catalase (CAT) 활성으로 평가하였다. 항균활성은 디스크 확산법에 의해 평가하였다. 결과: 대복피 80% MeOH 추출물 및 n-hexane, EtOAc, n-butanol, H2O 용매 분획물의 추출 수율의 결과, 각각 19.04, 14.33, 12.07, 22.53, 50.97%로 측정되었다. 총 폴리페놀 함량은 80% MeOH 추출물에서 16.6 mg/g으로 가장 높게 측정되었다. DPPH free radical 소거능은 n-hexane 분획물에서 102.9 mg/mL로 양성대조군인 vitamin C 및 butylated hydroxytoluene (BHT)와 비슷한 우수한 항산화 활성을 나타내었다. SOD 활성은 EtOAc 분획물에서가장 높은 값(38.73 U mg protein-1)을 나타내었다. CAT 활성은 n-butanol 분획물에서 가장 높은 값(2.75 U mg protein-1 min-1) 을 나타내었다. 항균활성의 결과에서, 대복피 80% MeOH 추출물은 8종의 균주에 대해 항균활성을 나타냈다.- nHexane 분획물은오직 Listeria monocytogenes에 대해서만 항균활성을 나타냈다. EtOAc, n-butanol, H2O 분획물에서는 8종 균주에 대하여 항균활성을 나타내지 않았다. 결론: 이상의 결과에서 대복피 80% MeOH 추출물은 천연 항산화 및 항균 소재로서의 활용이 가능할 것으로 기대된다.
Abstract
Purpose: This present study was performed to investigate anti-oxidant and anti-bacterial activities of Arecae pericarpium MeOH extracts and its solvent fractions. Methods: Arecae pericarpium was extracted by 80% MeOH and fractionated by n-hexane, ethyl acetate (EtOAc), n-butanol, and H2O. Free radical scavenging activities on 80% MeOH Arecae pericarpium extracts and its fractions were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Anti-oxidant effects were confirmed by catalase (CAT) and superoxide dismutase (SOD) activities. Anti-bacterial activity was evaluated by the paper disk diffusion method. Results: Extraction yields of 80% MeOH Arecae pericarpium extracts and its fractions such as n-hexane, EtOAc, n-butanol, and H2O, were 19.04, 14.33, 12.07, 22.53, and 50.97%, respectively. The 80% MeOH extracts got the highest value (16.6 mg/g) in total polyphenol contents. In the case of DPPH free radical scavenging activity, the n-hexane fraction was 102.9 mg/mL. It was similar to the value of positive control, vitamin C and butylated hydroxytoluene (BHT). The highest values of SOD and CAT activities were confirmed at the EtOAc fraction (38.73 U mg protein-1) and the n-butanol fraction (2.75 U mg protein-1 min-1), respectively. In the results of anti-bacterial activity, 80% MeOH Arecae pericarpium extracts had anti-bacterial effects on all eight microorganisms. The n-hexane fraction had the anti-bacterial effects on only Listeria monocytogenes. There are no anti-bacterial effects on the other fractions such as EtOAc, n-butanol, and H2O. Conclusion: It is considered that these results imply the 80% MeOH Arecae pericarpium extracts can be used as not only natural antioxidants but also natural antibacterials.
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