구실잣밤나무 추출물의 항산화 및 항균 활성
Antioxidative and Antimicrobial Activities of Castanopsis cuspidata var. sieboldii Extracts
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초록
본 연구는 구실잣밤나무를 식품 저장성이나 안전성을 향상하기 위한 식품 보존제로서의 개발 가능성을 알아보고자 하였다. 구실잣밤나무의 잎을 에탄올로 추출하고 헥산, 디클로로메탄, 에틸아세테이트, 부탄올로 순차적으로 용매분획 하였다. 먼저, 항산화활성으로 DPPH 소거활성, superoxide radical 소거 활성 그리고 xanthine oxidase 억제 활성을 측정하였다. 그 결과, 에틸아세테이트 분획물에서 농도 의존적으로 가장 높은 항산화 활성을 나타내었다. 항균활성은 에탄올 추출물과 용매분획물을 농도별로 조사한 결과 다른 분획물에 비해 Bacillus sublitis, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Salmonella enteritidis 그리고 Salmonella typhimurium에서 에틸아세테이트 분획물에 높은 활성을 띠었다. 이상의 결과를 볼 때, 구실잣밤나무 잎 추출물은 식품 보존제의 개발에 적합 할 수 있다고 사료 된다.
Abstract
This study was designed to investigate the possible utilization of Castanopsis cuspidata as a source of antiseptic agents. The leaves of C. cuspidata, extracted by 80% ethanol, were sequentially fractionated with n-hexane, dichloromethane, ethylacetate, and n-butanol. In order to effectively screen for a natural preservative agent, we first investigated the antioxidant activities such as DPPH radical scavenging capacity, superoxide radical scavenging capacity, and xanthine oxidase inhibitory activity of the C. cuspidata ethanol extracts and fraction. Using a screening system, we found that the ethylacetate fraction had the strongest antioxidant activity, which followed a dose-dependent manner. The antimicrobial activities were shown in the ethylacetate fraction of C. cuspidata. Among the five fractions, the ethylacetate fraction showed the highest antimicrobial activities against microorganisms tested, which were Bacillus sublitis, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Salmonella enteritidis and Salmonella typhimurium. In addition, leaf extracts of C. cuspidata could be suitable for the development of food preservatives.
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