율피 추출물 및 분획물의 항산화 활성
Antioxidant activity of ethanol extracts and fractions from Castanea crenata inner shell
2
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
This study examined the antioxidant activity of ethanol extract and fraction from Castanea crenata inner shell in vitro. Antioxidant activity was evaluated through total polyphenol and flavonoid contents and DPPH radical scavenging ability using electron spin resonance (ESR). The total polyphenol contents of the 70% ethanol extract and ethyl acetate fraction were 39.76 mg GAE/g and 323.92 mg GAE/g (p < 0.05). The total polyphenol contents of the ethyl acetate fraction were higher than other fractions. Furthermore, the total flavonoid contents of ethyl acetate were higher than other fractions at 13.12 mg QE/g (p < 0.05). In DPPH radical scavenging ability measurement, the RC50 value of ethyl acetate was lower than ascorbic acid at 2.27 μg/mL (p < 0.05). In conclusion, this result showed that the ethyl acetate fraction of Castanea crenata inner shell 70% ethanol extract has high antioxidant activity.
Abstract
This study examined the antioxidant activity of ethanol extract and fraction from Castanea crenata inner shell in vitro. Antioxidant activity was evaluated through total polyphenol and flavonoid contents and DPPH radical scavenging ability using electron spin resonance (ESR). The total polyphenol contents of the 70% ethanol extract and ethyl acetate fraction were 39.76 mg GAE/g and 323.92 mg GAE/g (p < 0.05). The total polyphenol contents of the ethyl acetate fraction were higher than other fractions. Furthermore, the total flavonoid contents of ethyl acetate were higher than other fractions at 13.12 mg QE/g (p < 0.05). In DPPH radical scavenging ability measurement, the RC50 value of ethyl acetate was lower than ascorbic acid at 2.27 μg/mL (p < 0.05). In conclusion, this result showed that the ethyl acetate fraction of Castanea crenata inner shell 70% ethanol extract has high antioxidant activity.
참고문헌 (25)
- Brieger K, "Reactive oxygen species: from health to disease", Swiss medical weekly, 142, pp.w13659~ (2012)
- Cha SH, "Screening for DPPH Free Radical Scavenging Activities of Autogenous Seaweeds in Jeju Island Using a Electron Spin Resonance (ESR) Spectroscopy", Journal of Marine Bioscience and Biotechnology, 1, pp.252~259 (2006)
- 최미옥, "유산균 발효에 의한 율피(Castanea crenata inner shell) 열수추출물의 아토피 피부 질환에 관한 효과 연구", 한국식품저장유통학회지, 20(4), pp.583~591 (2013), 한국식품저장유통학회
- Cizmarova B, "Pycnogenol - extract from French maritime pine bark (Pinus pinaster), as an effective antioxidant against superoxide radical", Functional Food Science, 1, pp.14~22 (2021)
- Frankel EN, "Antioxidants in lipid foods and their impact on food quality", Food chemistry, 57, pp.51~55 (1996)
- Galanakis CM, "A Knowledge Base for The Recovery of Natural Phenols with Different Solvents", International Journal of Food Properties, 16, pp.382~396 (2013)
- Ganesan K, "Polyphenol-Rich Lentils and Their Health Promoting Effects", International journal of molecular sciences, 18, pp.2390~ (2017)
- Garcia-Conesa MT, "Polyphenol-Rich Foods for Human Health and Disease", Nutrients, 12, pp.400~ (2020)
- 구율리, "효소처리 및 가압추출 공정을 이용한 율피 추출물의항산화, 피부 미백 및 주름개선 효과", 한국식품저장유통학회지, 25(1), pp.79~89 (2018), 한국식품저장유통학회
- He W, "Electron spin resonance spectroscopy for the study of nanomaterial-mediated generation of reactive oxygen species", Journal of Food and Drug Analysis, 22, pp.49~63 (2014)
- 장민정, "밤부산물(율피)의 주름개선 및 미백 효과", 생명과학회지, 21(5), pp.734~738 (2011), 한국생명과학회
- Kim CY, "Inner Shell of Chestnut (Castanea crenatta) Supresses Inflammatory Responses in Ovalbumin-Induced Allergic Asthma Mouse Model", Nutrients, 14, pp.2067~ (2022)
- 김진윤, "밤 과육, 껍질 및 밤나무 잎 추출물에 대한 항산화 및 항염증 활성 비교", 한국약용작물학회지, 22(1), pp.8~16 (2014), 한국약용작물학회[DOI]
- 김경철, "굴거리 열매 추출물의 폴리페놀 함량과 항산화 활성", 공학기술논문지, 12(4), pp.149~155 (2019), 공학기술연구원[DOI]
- 김숙진, "울릉도산 식물의 HepG2 세포에서 지질 축적 및 산화 스트레스 개선 효과", 한국식품저장유통학회지, 27(6), pp.817~828 (2020), 한국식품저장유통학회
- 김유진, "차전초 에탄올 추출물의 항산화 효능", 대한본초학회지, 33(3), pp.37~43 (2018), 대한본초학회[DOI]
- Kwon EJ, "Antioxidative Activity of Solvent Fraction and Isolation of Antioxidative Compound from Chestnut Husk", Journal of the Korean Society of Food Science and Nutrition, 30, pp.726~731 (2001)
- Duc Dat Le, "Quantitation and Radical Scavenging Activity Evaluation of Iridoids and Phenylethanoids from the Roots of Phlomis umbrosa (Turcz.) using DPPH Free Radical and DPPH-HPLC Methods, and their Cytotoxicity", Natural Product Sciences, 25(2), pp.122~129 (2019), 한국생약학회[DOI]
- 이지예, "율피분말 첨가가 양갱의 이화학적 및 관능적 특성에 미치는 영향", 한국식품조리과학회지, 37(1), pp.31~40 (2021), 한국식품조리과학회
- 이민영, "과일 껍질의 비타민 C , 폴리페놀, 플라보노이드 함량과 항산화 활성", 한국식품과학회지, 44(5), pp.540~544 (2012), 한국식품과학회[DOI]
- 김남우, "싸리(Lespedeza bicolor) 추출물의 항산화성에 관한 연구", 한국식품저장유통학회지, 12(1), pp.75~79 (2005), 한국식품저장유통학회
- Nawaz H, "Effect of solvent polarity on extraction yield and antioxidant properties of phytochemicals from bean (Phaseolus vulgaris) seeds", Brazilian Journal of Pharmaceutical Sciences, 56, pp.e17129~ (2020)
- Noh JR, "Hepatoprotective effects of chestnut(Castanea crenata)inner shell extract against chronic ethanol-induced oxidative stress in C57BL", 6 mice, 2011, pp.49, 1537~1543
- 박초희, "포도즙을 첨가한 양갱의 품질특성 및 유리기 소거능", 한국식품영양학회지, 27(4), pp.596~602 (2014), 한국식품영양학회
- Sajid M, "Synthetic Biology towards Improved Flavonoid Pharmacokinetics", Biomolecules, 11, pp.754~ (2021)