굴거리 열매 추출물의 폴리페놀 함량과 항산화 활성
Polyphenol Contents and Antioxidant Activity of Fruit Extracts from Daphniphyllum macropodum
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초록
As an effort to develop functional ingredients and to discover their antioxidant activity, the total polyphenol content, total flavonoid content, 2,2-diphenyl-1-pcrylhydrazyl (DPPH) radical scavenging activity, trolox equivalent antioxidant capacity (TEAC), oxygen radical absorption capacity (ORAC), and ferric reducing antioxidant power (FRAP) of Daphniphyllum macropodum were measured using a methanol extract and various solvent fractions. Based on the result, the total polyphenol content was highest in an ethyl acetate fraction of 212.33±1.05 mg GAE/g where the total flavonoid content was 77.96±0.21 mg QE/g. The DPPH radical scavenging activity was highest in an ethyl acetate fraction (IC50 : 47.10±1.32 μg/mL). The ethyl acetate fraction showed the highest TEAC (1052.23±13.94 mM TE/g), ORAC (9308.71±480.69 mM TE/g), and FRAP (2064.76±42.31 mM FE/g) in antioxidant capacity value. The total polyphenol and flavonoid contents significantly correlated with the antioxidant activity of D. macropodum extracts and fractions. In addition, the survival rate of HaCaT and HepG2 cells at 200 μg/mL was higher than 70%. With these results, it was confirmed that the ethyl acetate fraction of D. macropodum has great antioxidant potential. Thus, it can be expected to be developed into a specific functional ingredient.
Abstract
As an effort to develop functional ingredients and to discover their antioxidant activity, the total polyphenol content, total flavonoid content, 2,2-diphenyl-1-pcrylhydrazyl (DPPH) radical scavenging activity, trolox equivalent antioxidant capacity (TEAC), oxygen radical absorption capacity (ORAC), and ferric reducing antioxidant power (FRAP) of Daphniphyllum macropodum were measured using a methanol extract and various solvent fractions. Based on the result, the total polyphenol content was highest in an ethyl acetate fraction of 212.33±1.05 mg GAE/g where the total flavonoid content was 77.96±0.21 mg QE/g. The DPPH radical scavenging activity was highest in an ethyl acetate fraction (IC50 : 47.10±1.32 μg/mL). The ethyl acetate fraction showed the highest TEAC (1052.23±13.94 mM TE/g), ORAC (9308.71±480.69 mM TE/g), and FRAP (2064.76±42.31 mM FE/g) in antioxidant capacity value. The total polyphenol and flavonoid contents significantly correlated with the antioxidant activity of D. macropodum extracts and fractions. In addition, the survival rate of HaCaT and HepG2 cells at 200 μg/mL was higher than 70%. With these results, it was confirmed that the ethyl acetate fraction of D. macropodum has great antioxidant potential. Thus, it can be expected to be developed into a specific functional ingredient.
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