항산화활성 비교를 통한 발효 칠피의 추출용매 조건 탐색
Screening of Extraction Solvent Condition of Fermented Rhus verniciflua Stem Bark by Antioxidant Activities
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초록
In this study, we investigated antioxidant capacity and determined the phenolic and flavonoid contents using each of various solvent conditions from fermented Rhus verniciflua stem bark (F-RVS). Each extracts displayed markedly similar content of extraction yield. However, M80 extract showed a significantly higher antioxidant activity in comparison to other extract investigated. M80 exhibited strong DPPH radical scavenging activity with RC50 value of 10.5 ± 1.4 ㎍/㎖,reducing power value 1.04 Abs (concentration of 1 ㎎/㎖), and anti-lipid peroxidation activity value of 94.6% (concentration of 10 ㎎/㎖). M80 extract showed the high content of total phenolic (319.7 ㎎ GAE/㎖ extract) and total flavonoid (111.6 ㎎QE/㎖ extract). Phenolic and flavonoid compounds showed significant relationship in DPPH radical scavenging (R2= 0.911and 0.912, each extract) and reducing power (R2 = 0.903 and 0.837, each extract) from fermented R. verniciflua stem bark. However, antilipid peroxidation activity (R2 = 0.589 and 0.441, each extract) was not significant like DPPH radical scavenging and reducing power. Therefor the result indicated that the potential antioxidant activities and functional values were ovserved significantly at M80 solvent condition from the fermented R. verniciflua stem bark.
Abstract
In this study, we investigated antioxidant capacity and determined the phenolic and flavonoid contents using each of various solvent conditions from fermented Rhus verniciflua stem bark (F-RVS). Each extracts displayed markedly similar content of extraction yield. However, M80 extract showed a significantly higher antioxidant activity in comparison to other extract investigated. M80 exhibited strong DPPH radical scavenging activity with RC50 value of 10.5 ± 1.4 ㎍/㎖,reducing power value 1.04 Abs (concentration of 1 ㎎/㎖), and anti-lipid peroxidation activity value of 94.6% (concentration of 10 ㎎/㎖). M80 extract showed the high content of total phenolic (319.7 ㎎ GAE/㎖ extract) and total flavonoid (111.6 ㎎QE/㎖ extract). Phenolic and flavonoid compounds showed significant relationship in DPPH radical scavenging (R2= 0.911and 0.912, each extract) and reducing power (R2 = 0.903 and 0.837, each extract) from fermented R. verniciflua stem bark. However, antilipid peroxidation activity (R2 = 0.589 and 0.441, each extract) was not significant like DPPH radical scavenging and reducing power. Therefor the result indicated that the potential antioxidant activities and functional values were ovserved significantly at M80 solvent condition from the fermented R. verniciflua stem bark.
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