전자선 조사에 의한 신선굴(Crassostrea gigas) 중 휴먼노로바이러스 GⅡ. 4의 저감화
Inactivation of Human Norovirus GII. 4 on Oyster Crassostrea gigas by Electron Beam Irradiation
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
This study investigated the reduction in human norovirus (HNV) GII. 4 count in pacific oyster Crassostrea gigas using electron beam irradiation. Infectious HNV GII. 4 was detected using RT-qPCR (real time reverse transcriptionquantitative polymerase chain reaction) with PMA (propidium monoazide)/sarkosyl. At electron beam doses 1, 5, 7, and 10 kGy, the count of HNV GII. 4 was 2.74, 2.37, 2.06, and 1.55 log copies/μL (control, 3.01 log copy/μL), respectively, confirming that as the irradiation dose increased, norovirus count reduced significantly (P<0.05). After PMA/ sarkosyl treatment, the counts further reduced at the same irradiation dose, and 10 kGy showed significant differences between the non-treated and PMA/sarkosyl-treated samples (P<0.05). The Ed (decimal reduction dose of electron beam) value based on the first-order kinetic model was 7.33 kGy (R2=0.98). No significant difference was observed in the pH values of the control (6.2) and electron beam-irradiated samples at all doses (6.1). For sensory evaluation, the non-treated sample scored the highest in all categories (5.25-6.17), while the samples treated with 10 kGy showed the lowest score (4.67-5.33), although without statistical significance (P>0.05). Overall, our results suggest that 7 kGy electron beam is sufficient for the non-thermal sterilization of oysters without causing significant changes in quality.
Abstract
This study investigated the reduction in human norovirus (HNV) GII. 4 count in pacific oyster Crassostrea gigas using electron beam irradiation. Infectious HNV GII. 4 was detected using RT-qPCR (real time reverse transcriptionquantitative polymerase chain reaction) with PMA (propidium monoazide)/sarkosyl. At electron beam doses 1, 5, 7, and 10 kGy, the count of HNV GII. 4 was 2.74, 2.37, 2.06, and 1.55 log copies/μL (control, 3.01 log copy/μL), respectively, confirming that as the irradiation dose increased, norovirus count reduced significantly (P<0.05). After PMA/ sarkosyl treatment, the counts further reduced at the same irradiation dose, and 10 kGy showed significant differences between the non-treated and PMA/sarkosyl-treated samples (P<0.05). The Ed (decimal reduction dose of electron beam) value based on the first-order kinetic model was 7.33 kGy (R2=0.98). No significant difference was observed in the pH values of the control (6.2) and electron beam-irradiated samples at all doses (6.1). For sensory evaluation, the non-treated sample scored the highest in all categories (5.25-6.17), while the samples treated with 10 kGy showed the lowest score (4.67-5.33), although without statistical significance (P>0.05). Overall, our results suggest that 7 kGy electron beam is sufficient for the non-thermal sterilization of oysters without causing significant changes in quality.
참고문헌 (31)
- Erwin Duizer, "Laboratory efforts to cultivate noroviruses", Journal of General Virology, 85(1), pp.79~87 (2004), Microbiology Society[DOI]
- Louise M. Fielding, "The effect of electron beam irradiation, combined with acetic acid, on the survival and recovery of Escherichia coli and Lactobacillus curvatus", International Journal of Food Microbiology, 35(3), pp.259~265 (1997), Elsevier BV[DOI]
- Noemí Fuster, "Propidium monoazide RTqPCR assays for the assessment of hepatitis A inactivation and for a better estimation of the health risk of contaminated waters", Water Research, 101, pp.226~232 (2016), Elsevier BV[DOI]
- Jay JJ, "Modern Food Microbiology", pp.1~277 (1996), Chapman Hall
- 제길수, "저선량 감마선 반복조사의 육류와 생선 중 미생물 저감효과", 한국식품위생안전성학회지, 30(1), pp.92~97 (2015), 한국식품위생안전성학회
- Eun Bi Jeon, "Characterizing the effects of thermal treatment on human norovirus GII.4 viability using propidium monoazide combined with RT-qPCR and quality assessments in mussels", Food Control, 109, pp.106954~ (2020), Elsevier BV[DOI]
- Myeong-In Jeong, "Thermal inactivation of human norovirus on spinach using propidium or ethidium monoazide combined with real-time quantitative reverse transcription-polymerase chain reaction", Food Control, 78, pp.79~84 (2017), Elsevier BV[DOI]
- 정사무엘, "전자선 조사 명란젓갈의 미생물학적, 이화학적 및 관능적 품질특성", Food Science and Preservation, 16(2), pp.198~203 (2009), 한국식품저장유통학회
- 김빛나, "전자선 조사 양념 오징어 젓갈 및 부재료의 미생물학적 및 관능적 특성", Korean Journal of Agricultural Science, 35(2), pp.155~165 (2008), 농업과학연구소
- 김신영, "Propidium monoazide와 real-time PCR을 이용한 살아있는 Enterococcus faecalis의 선택적인 검출", Restorative Dentistry and Endodontics, 33(6), pp.537~544 (2008), 대한치과보존학회
- 김미선, "감마선 조사에 의한 복숭아의 미생물학적, 이화학적 품질 및 관능적 품질 변화", 한국식품영양과학회지, 38(3), pp.364~371 (2009), 한국식품영양과학회
- 김현주, "UV-C 및 전자선 조사가 막걸리의 품질에 미치는 영향", Food Science and Preservation, 20(1), pp.45~51 (2013), 한국식품저장유통학회
- 김유진, "전자선 처리에 따른 마른 김(Porphyra tenera)의 미생물 저감화 효과와 저항성 세균의 동정", Food Science and Preservation, 23(1), pp.139~143 (2016), 한국식품저장유통학회
- Seh Eun Kim, "Effects of electron beam irradiation on murine norovirus-1 in abalone (Haliotis discus hannai) meat and viscera", LWT, 86, pp.611~618 (2017), Elsevier BV[DOI]
- Lee MH, "Detection of norovirus using molecular biological method", Safe Food, 9, pp.26~32 (2014)
- Hae-Won Lee, "Pretreatment with propidium monoazide", sodium lauroyl sarcosinate improves discrimination of infectious waterborne virus by RT-qPCR combined with magnetic separation, 2018, pp.233, 306~314[DOI]
- Hae-Won Lee, "Use of RT-qPCR with combined intercalating dye and sodium lauroyl sarcosinate pretreatment to evaluate the virucidal activity of halophyte extracts against norovirus", Food Control, 98, pp.100~106 (2019), Elsevier BV[DOI]
- David Lees, "Viruses and bivalve shellfish", International Journal of Food Microbiology, 59(1-2), pp.81~116 (2000), Elsevier BV[DOI]
- "Statistics of food poisoning" (2020)[URL]
- L. Moreno, "Application of viability PCR to discriminate the infectivity of hepatitis A virus in food samples", International Journal of Food Microbiology, 201, pp.1~6 (2015), Elsevier BV[DOI]
- Tania Nolan, "Quantification of mRNA using real-time RT-PCR", Nature Protocols, 1(3), pp.1559~1582 (2006), Springer Science and Business Media LLC[DOI]
- Park JS, "Microbiological, physicochemical, and sensory characteristics of gamma-irradiated fresh oysters during storage", J Radiat Ind, 2, pp.85~91 (2008)
- Shin Young Park, "Influence of NaCl on the inactivation of murine norovirus-1 and hepatitis A virus in the Korean traditional salted oyster product “Eoriguljeot” during storage", Food Research International, 62, pp.382~387 (2014), Elsevier BV[DOI]
- 박준석, "조미 자숙굴(Crassostrea gigas) 통조림 및 조미 구운굴(Crassostrea gigas) 통조림의 제조 및 품질특성", 한국수산과학회지, 51(5), pp.469~476 (2018), 한국수산과학회[DOI]
- Shin Young Park, "Synergistic Effects of Combined Chlorine and Vitamin B1 on the Reduction of Murine Norovirus-1 on the Oyster (Crassostrea gigas) Surface", Food and Environmental Virology, 11(3), pp.205~213 (2019), Springer Science and Business Media LLC[DOI]
- Chandni Praveen, "Susceptibility of Murine Norovirus and Hepatitis A Virus to Electron Beam Irradiation in Oysters and Quantifying the Reduction in Potential Infection Risks", Applied and Environmental Microbiology, 79(12), pp.3796~3801 (2013), American Society for Microbiology[DOI]
- Ashley Predmore, "Electron beam inactivation of Tulane virus on fresh produce, and mechanism of inactivation of human norovirus surrogates by electron beam irradiation", International Journal of Food Microbiology, 198, pp.28~36 (2015), Elsevier BV[DOI]
- Knut Rudi, "Use of Ethidium Monoazide and PCR in Combination for Quantification of Viable and Dead Cells in Complex Samples", Applied and Environmental Microbiology, 71(2), pp.1018~1024 (2005), American Society for Microbiology[DOI]
- 신순범, "통영시 연안의 양식굴(Crassostrea gigas)에서 검출된 노로바이러스의 정량분석", 한국수산과학회지, 47(5), pp.501~507 (2014), 한국수산과학회
- 손광태, "굴의 선도에 따른 pH, 글리코겐, 가용성단백질, 탁도와의 상관관계", 한국수산과학회지, 47(5), pp.495~500 (2014), 한국수산과학회
- 송범석, "감마선 또는 전자선 조사된 분쇄돈육과 돈육패티의 저장 중 품질특성 비교", 한국축산식품학회지, 29(2), pp.194~202 (2009), 한국축산식품학회