가열처리 조건에 따른 오염굴(Crassostrea gigas) 중의 Male Specific Coliphage와 노로바이러스 농도변화
Effect of Heat Treatment on Male specific Coliphage and Norovirus Concentrations in Norovirus Contaminated Oyster Crassostrea gigas
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초록
Noroviruses (NoV) are known to cause acute epidemic gastroenteritis worldwide. Outbreak strains are predominantly genogroup I (GI) and genogroup II (GII) in oysters Crassostrea gigas. We investigated the changes in concentration of male specific coliphage (MSC) and NoV under heat treatment of the naturally contaminated oyster, Crassostrea gigas. After heat treatment for 5 min in 85℃, no viable MSC was detected. The concentrations of GI and GII NoV decreased by 1.65 log and 2.25 log, respectively, following heat treatment for 5 min at 100℃. Moreover, both GI and GII NoV were completely deactivated by heat treatment for 10 min at 100℃. Therefore, in order to reduce the risk of norovirus infection from contaminated oysters, immersion in boiling water for at least 10 min is recommended.
Abstract
Noroviruses (NoV) are known to cause acute epidemic gastroenteritis worldwide. Outbreak strains are predominantly genogroup I (GI) and genogroup II (GII) in oysters Crassostrea gigas. We investigated the changes in concentration of male specific coliphage (MSC) and NoV under heat treatment of the naturally contaminated oyster, Crassostrea gigas. After heat treatment for 5 min in 85℃, no viable MSC was detected. The concentrations of GI and GII NoV decreased by 1.65 log and 2.25 log, respectively, following heat treatment for 5 min at 100℃. Moreover, both GI and GII NoV were completely deactivated by heat treatment for 10 min at 100℃. Therefore, in order to reduce the risk of norovirus infection from contaminated oysters, immersion in boiling water for at least 10 min is recommended.
참고문헌 (21)
- Arcangeli G, "Effect of high hydrostatic pressure on murine norovirus in Manila clams", Lett Appl Microbiol, 54, pp.325~329 (2012)[DOI]
- Alfano-Sobsey E, "Norovirus outbreak associated with undercooked oysters and secondary household transmission", Epidemiol Infect, 140, pp.276~282 (2012)[DOI]
- Blackburn, BG, "Surveillance for waterborne-disease outbreaks associated with drinking water-United States, 2001-2002", MMWR Surveill Summ, 53, pp.23~45 (2004)
- Burkhardt W 3rd, "Survival and replication of male-specific bacteriophages in molluscan shellfish", Appl Environ Microbiol, 58, pp.1371~1373 (1992)
- Microbial indicator levels in shellfish, water, and sediments from the upper Narragansett Bay conditional shellfish-growing area. Report to the Narragansett Bay Project, "Cabelli VJ.", Narragansett Bay Project, ~ (1988)
- Croci L, "Inactivation of hepatitis A virus in heat-treated mussels", J Appl Microbiol, 87, pp.884~888 (1999)
- Hewitt J, "Effect of heat treatment on hepatitis A virus and norovirus in New Zealand greenshell mussels(Perna canaliculus)by quantitative real-time reverse transcription PCR and cell culture", J Food Prot, 69, pp.2217~2223 (2006)
- Iritani N, "Detection and genetic characterization of human enteric viruses in oysterassociated gastroenteritis outbreaks between 2001 and 2012 in Osaka City, Japan", J Med Virol, 86, pp.2019~2025 (2014)[DOI]
- Jothikumar N, "Rapid and sensitive detection of noro-viruses by using TaqMan-based one-step reverse transcription-PCR assays and application to naturally contaminated shellfish samples", Appl Environ Microbiol, 71, pp.1870~1875 (2005)
- Koopmans M, "Foodborne viruses", FEMS Microbiol, 26, pp.187~205 (2002)
- 권지영, "Evalution of the Bacteriological Quality of a Shellfish-growing Area in Kamak Bay, Korea", Fisheries and Aquatic Sciences, 11(1), pp.7~14 (2008), 한국수산과학회[DOI]
- Lowther JA, "Two-year systematic study to assess norovirus contamination in oysters from commercial harvesting area in the United Kingdom", Appl Environ Microbiol, 78, pp.5812~5817 (2012)
- 문애리, "Prevalence of Noroviruses in Oysters in Korea", Food Science and Biotechnology, 20(4), pp.1151~1154 (2011), 한국식품과학회
- 박큰바위, "강우 발생에 따른 남해군 봉천 방출수가 강진만 해역의 세균학적 수질에 미치는 영향 평가", 한국수산과학회지, 44(6), pp.622~629 (2011), 한국수산과학회[DOI]
- 박큰바위, "강우 발생에 따른 육상오염원이 나로도 해역의 세균학적 수질에 미치는 영향 평가", 한국수산과학회지, 45(5), pp.414~422 (2012), 한국수산과학회[DOI]
- 신순범, "Genetic Diversity of Noroviruses Detected in Oysters in Jinhae Bay,Korea", Food Science and Biotechnology, 22(5), pp.1453~1460 (2013), 한국식품과학회[DOI]
- 신순범, "통영시 연안의 양식굴(Crassostrea gigas)에서 검출된 노로바이러스의 정량분석", 한국수산과학회지, 47(5), pp.501~507 (2014), 한국수산과학회
- Simmons G, "A New Zealand outbreak of norovirus gastroenteritis linked to the consumption of imported raw Korean oysters", N Z Med J, 120, pp.U2773~ (2007)
- Sow H, "Heat inactivation of hepatitis A virus and a norovirus surrogate in soft-shell clams (Mya arenaria)", Foodborne Pathog Dis, 8, pp.387~393 (2011)[DOI]
- Ueki Y, "Persistence of caliciviruses in artificially contaminated oysters during depuration", Appl Environ Microbiol, 73, pp.5698~5701 (2007)
- Webby RJ, "Internationally distributed frozen oyster meat causing multiple outbreaks of norovirus infection in Australia", Clin Infect Dis, 44, pp.1026~1031 (2007)
