통영시 연안의 양식굴(Crassostrea gigas)에서 검출된 노로바이러스의 정량분석
Norovirus Quantification in Oysters Crassostrea gigas Collected from Tongyeoung, Korea
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초록
Norovirus (NoV) is a major cause of food poisoning outbreaks in Korea. Most NoV outbreaks originate from environmentalcontamination, but bivalves such as oysters are also important vectors. Oyster Crassostrea gigas contaminationby NoV has been reported in Korea, but no quantitative analyses of NoV have been performed. We investigatedthe NoV concentration in 21 oyster samples from a Korean commercial oyster-growing area with confirmedfecal contamination from January to December 2012, using real-time reverse transcription–polymerase chain reaction. Additionally, we assessed the NoV concentration after heating to investigate the effects of heat treatment onNoV-infected oysters. In NoV-positive samples, the cycle threshold (Ct) values were 37.43-39.41 and 36.77-39.30,while viral concentrations were 8.97×102–2.24×102 and 3.05×102–7.47×101 copies/g for genogroups I and II, respectively. After heat treatment, NoV genogroup I decreased by 83.4%, 88.0%, 89.4% and 100% at 60°C, 68°C, 70°C,and 100°C, respectively, for 15 min, while genogroup II respectively decreased by 67.3%, 76.3%, 80.1%, and 89.8%under the same conditions.
Abstract
Norovirus (NoV) is a major cause of food poisoning outbreaks in Korea. Most NoV outbreaks originate from environmentalcontamination, but bivalves such as oysters are also important vectors. Oyster Crassostrea gigas contaminationby NoV has been reported in Korea, but no quantitative analyses of NoV have been performed. We investigatedthe NoV concentration in 21 oyster samples from a Korean commercial oyster-growing area with confirmedfecal contamination from January to December 2012, using real-time reverse transcription–polymerase chain reaction. Additionally, we assessed the NoV concentration after heating to investigate the effects of heat treatment onNoV-infected oysters. In NoV-positive samples, the cycle threshold (Ct) values were 37.43-39.41 and 36.77-39.30,while viral concentrations were 8.97×102–2.24×102 and 3.05×102–7.47×101 copies/g for genogroups I and II, respectively. After heat treatment, NoV genogroup I decreased by 83.4%, 88.0%, 89.4% and 100% at 60°C, 68°C, 70°C,and 100°C, respectively, for 15 min, while genogroup II respectively decreased by 67.3%, 76.3%, 80.1%, and 89.8%under the same conditions.
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