Negative Correlation between the Prevalence of Norovirus and High Bacterial Loads of Escherichia coli in Oysters Crassostrea gigas
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Seasonal variation in the prevalence of norovirus in oysters Crassostrea gigas was investigated and compared to levels of Escherichia coli, a fecal indicator in oysters. Oysters were collected from Iwon-myeon, Taean-gun, Korea, a primary production area for European Union export of oysters between 2013 and 2014. We observed seasonality in the prevalence of norovirus in oysters, with a higher prevalence and viral load detected during winter months. Oysters taken from production areas that complied with the European Union standard for raw consumption (< 230 MPN/100 g of E. coli) had 22.1% of the samples test positive for norovirus (15/68 samples). However, norovirus was not detected in any of the samples (0%, 0/4 samples) that were collected from production sites that exceeded the standard fecal contamination level for raw consumption (> 230 MPN/100 g of E. coli). These results indicated that there is a negative correlation between the prevalence of norovirus and high levels of E. coli in oysters. Therefore, our results suggest that current food safety guidelines using only a bacterial fecal contamination indicator, E. coli, may not adequately assess shellfish production areas for viral and bacterial contamination.
Abstract
Seasonal variation in the prevalence of norovirus in oysters Crassostrea gigas was investigated and compared to levels of Escherichia coli, a fecal indicator in oysters. Oysters were collected from Iwon-myeon, Taean-gun, Korea, a primary production area for European Union export of oysters between 2013 and 2014. We observed seasonality in the prevalence of norovirus in oysters, with a higher prevalence and viral load detected during winter months. Oysters taken from production areas that complied with the European Union standard for raw consumption (< 230 MPN/100 g of E. coli) had 22.1% of the samples test positive for norovirus (15/68 samples). However, norovirus was not detected in any of the samples (0%, 0/4 samples) that were collected from production sites that exceeded the standard fecal contamination level for raw consumption (> 230 MPN/100 g of E. coli). These results indicated that there is a negative correlation between the prevalence of norovirus and high levels of E. coli in oysters. Therefore, our results suggest that current food safety guidelines using only a bacterial fecal contamination indicator, E. coli, may not adequately assess shellfish production areas for viral and bacterial contamination.
참고문헌 (34)
- Allwood PB, "Effect of temperature on the survival of F-specific RNA coliphage, feline calicivirus, and Escherichia coli in chlorinated water", Int J Environ Res Public Health, 2, pp.442~446 (2005)
- Ang LH, "An outbreak of viral gastroenteritis associated with eating raw oysters", Commun Dis Public Health, 1, pp.38~40 (1998)
- Bagordo F, "Rotavirus occurrence in shellfish with low levels of E. coli", Food Environ Virol, 5, pp.169~175 (2013)
- Blanton LH, "Molecular and epidemiologic trends of caliciviruses associated with outbreaks of acute gastroenteritis in the United States, 2000–2004", J Infect Dis, 193, pp.413~421 (2006)
- Cannon JL, "Surrogates for the study of norovirus stability and inactivation in the environment : a comparison of murine norovirus and feline calicivirus", J Food Prot, 69, pp.2761~2765 (2006)
- Carlos JA, "Environmental influences on faecal indicator organisms in coastal waters and their accumulation in bivalve shellfish estuaries and coasts", Est Coast, 36 (2013)
- Chalmers JWT, "An outbreak of viral gastroenteritis associated with adequately prepared oysters", Epidemiol Infect, 115, pp.163~167 (1995)
- Clements K, "Epizoic barnacles act as pathogen reservoirs on shellfish beds", J Shellfish Res, 32, pp.533~538 (2013)
- Dancer D, "Human norovirus RNA persists in seawater under simulated winter conditions but does not bioaccumulate efficiently in Pacific oysters (Crassostrea gigas)", J Food Prot, 73, pp.2123~2127 (2010)
- Diez-Valcarce M, "Occurrence of human enteric viruses in commercial mussels at retail level in three European countries", Food Environ Virol, 4, pp.73~80 (2012)
- Donia D, "Presence of hepatitis E RNA in mussels used as bio-monitors of viral marine pollution", J Virol Methods, 186, pp.198~202 (2012)
- Doré WJ, "The development of management strategies for control of virological quality in oysters", Water Sci Technol, 38, pp.29~35 (1998)
- European Community, "Regulation (EC) no 854", 2004 of the European Parliament and of the Council of 29 April 2004 laying down specific rules for the organisation of official controls on products of animal origin intended for human consumption, 2004, pp.L226, 83~127
- Flannery J, "Use of FRNA bacteriophages to indicate the risk of norovirus contamination in Irish oysters", J Food Prot, 72, pp.2358~2362 (2009)
- Fong TT, "Enteric viruses of human and animals in aquatic environments : health risks, detection, and potential water quality assessment tools", Microbiol Mol Biol Rev, 69, pp.357~371 (2005)
- Formiga-Cruz M, "Distribution of human virus contamination in shellfish from different growing areas in Greece, Spain, Sweden, and the United Kingdom", Appl Environ Microbiol, 68, pp.5990~5998 (2002)
- Gerba CP, "Comparative inactivation of enterovirus and adenovirus 2 by UV light", Appl Environ Microbiol, 68, pp.5167~5169 (2002)
- Greening GE, "Norovirus detection in shellfish using a rapid, sensitive virus recovery and real-time RT-PCR detection protocol", Food Analytical Methods, 1, pp.109~118 (2008)
- Greening GE, "Enteric viruses management of shellfish production in New Zealand", Food Environ Virol, 2, pp.167~175 (2010)
- Haramoto E, "Seasonal profiles of human noroviruses and indicator bacteria in a wastewater treatment plant in Tokyo, Japan", Water Sci Technol, 54, pp.301~308 (2006)
- International Organization for Standardization, "Microbiology of food and animal feeding stuffs horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli. Part 3. Most probable number technique using 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide", ISO, 2005
- Jiang S, "Human adenoviruses and coliphages in urban runoff-impacted coastal waters of Southern California", Appl Environ Microbiol, 67, pp.179~184 (2001)
- Kageyama T, "Broadly reactive and highly sensitive assay for Norwalk-like viruses based on real-time quantitative reverse transcription-PCR", J Clinic Microbiol, pp.1548~1557 (2003)
- Korea Food and Drug Administrati, "Guideline for investigation on the cause of food poisoning", pp.182~201 (2014), Korea Food and Drug Administration
- Le Guyader F, "Three-year study to assess human enteric viruses in shellfish", Appl Environ Microbiol, 66, pp.3241~3248 (2000)
- 이희정, "양전하 및 음전하 필터를 이용한 해수 중 Norovirus Surrogate의 회수", 한국수산과학회지, 42(3), pp.238~242 (2009), 한국수산과학회
- Lees DN, "Viruses in bivalve shellfish", Int J Food Microbiol, 59, pp.81~116 (2000)
- Lees DN, "International standardisation of a method for detection of human pathogenic viruses in molluscan shellfish(CENWG6TAG4). Food Environ Virol 2, 146 –155", Food Environ Virol, 2, pp.146~155 (2010)
- Legeay O, "Simplified procedure for detection of enteric pathogenic viruses in shellfish by RT-PCR", J Virol Methods, 90, pp.1~14 (2000)
- Lowther JA, "Two-year systematic study to assess norovirus contamination in oysters from commercial harvesting areas in the United Kingdom", Appl Environ Microbiol, 78, pp.5812~5817 (2012)
- Lowther JA, "Determination of norovirus contamination in oysters from two commercial harvesting areas over an extended period, using semiquantitative real-time reverse transcription PCR", J Food Prot, 71, pp.1427~1433 (2008)
- Manso CF, "Detection of hepatitis A virus and norovirus from Galicia(NW Spain)", Food Environ Virol, 5, pp.110~118 (2013)
- 신순범, "Genetic Diversity of Noroviruses Detected in Oysters in Jinhae Bay,Korea", Food Science and Biotechnology, 22(5), pp.1453~1460 (2013), 한국식품과학회[DOI]
- Troussellier M, "Responses of enteric bacteria to environmental stresses in seawater", Oceanologica Acta, 21, pp.965~981 (1998)