해수 중의 수온, 염분 및 pH에 따른 노로바이러스 및 Male Specific Coliphage 농도변화
Change in Concentrations of Human Norovirus and Male-Specific Coliphage under Various Temperatures, Salinities, and pH Levels in Seawater
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초록
Pre- or post-harvest processing is required to mitigate the risk of norovirus infection mediated by shellfish or seafood. We investigated the environmental resistance of human norovirus (HuNoV) under various conditions of temperature, salinity, and pH in seawater. Male-specific coliphage (MSC) was as the reference virus for all tests. At 4℃, HuNoV GII4 spiked into seawater was continually detected by RT-PCR for 35 days, regardless of salinity or pH level. It maintained nearly stable concentrations, meaning HuNoV can sustain a viral population in seawater long enough to be accumulated by shellfish and other filter feeders during winter. MSC was also stable at 4℃ although viral infectivity dropped sharply after 28 days. The effects of salinity and pH on MSC were indistinct. At 25℃ the detectable period of HuNoV GII4 by RT-PCR in seawater decreased to about one-third or half of the period at 4℃. High salinity (32 psu) and alkaline pH (8.5) were also unfavorable for sustaining HuNoV abundance at 25℃ in seawater. The resistance patterns of MSC to high temperature, high salinity, and alkaline pH were more dramatic and viral infectivity decreased over time, almost in direct proportion to experimental days. MSC was undetectable after 12 days under all salinities and pH levels at 25℃.
Abstract
Pre- or post-harvest processing is required to mitigate the risk of norovirus infection mediated by shellfish or seafood. We investigated the environmental resistance of human norovirus (HuNoV) under various conditions of temperature, salinity, and pH in seawater. Male-specific coliphage (MSC) was as the reference virus for all tests. At 4℃, HuNoV GII4 spiked into seawater was continually detected by RT-PCR for 35 days, regardless of salinity or pH level. It maintained nearly stable concentrations, meaning HuNoV can sustain a viral population in seawater long enough to be accumulated by shellfish and other filter feeders during winter. MSC was also stable at 4℃ although viral infectivity dropped sharply after 28 days. The effects of salinity and pH on MSC were indistinct. At 25℃ the detectable period of HuNoV GII4 by RT-PCR in seawater decreased to about one-third or half of the period at 4℃. High salinity (32 psu) and alkaline pH (8.5) were also unfavorable for sustaining HuNoV abundance at 25℃ in seawater. The resistance patterns of MSC to high temperature, high salinity, and alkaline pH were more dramatic and viral infectivity decreased over time, almost in direct proportion to experimental days. MSC was undetectable after 12 days under all salinities and pH levels at 25℃.
참고문헌 (24)
- E. ALFANO-SOBSEY, "Norovirus outbreak associated with undercooked oysters and secondary household transmission", Epidemiology and Infection, 140(02), pp.276~282 (2012), Cambridge University Press (CUP)[DOI]
- Allwood PB, "Survival of F-specific RNA coliphage, feline calicivirus and Escherichia coli in water : a comparative study", Appl Environ Micrbiol, 69, pp.5707~5710 (2003)
- G. Arcangeli, "Effect of high hydrostatic pressure on murine norovirus in Manila clams", Letters in Applied Microbiology, 54(4), pp.325~329 (2012), Wiley-Blackwell[DOI]
- Blackburn, BG, "Surveillance for waterborne-disease outbreaks associated with drinking water-United States. 2001-2002", MMWR Surveill Summ, 53, pp.23~45 (2004)
- Burkhardt W 3rd, "Survival and replication of male-specific bacteriophages in molluscan shellfish", Appl Environ Microbiol, 58, pp.1371~1373 (1992)
- Bull RA, "Emergence of a new norovirus genotype Ⅱ. 4 variant associated with global outbreaks of gastroenteritis", J Clin Microbiol, 44, pp.327~333 (2006)
- Cannon JL, "Surrogate for the study of norovirus stability and inactivation in the environment : A comparison of murine norovirus and feline calicivirus", J Food Protect, 69, pp.2761~2765 (2006)
- Chen H, "Temperature and treatment time influence high hydrostatic pressure inactivation of feline calicivirus, a norovirus surrogate", J Food Protect, 68, pp.2389~2394 (2005)
- DeBartolomeis J, "Evaluation of an Escherichia coli of F male-specific bacteriophages", Appl Environ Microbiol, 57, pp.1301~1305 (1991)
- Nobuhiro Iritani, "Detection and genetic characterization of human enteric viruses in oyster-associated gastroenteritis outbreaks between 2001 and 2012 in Osaka City, Japan", Journal of Medical Virology, 86(12), pp.2019~2025 (2014), Wiley-Blackwell[DOI]
- Jung S, "Occurrence of Norovirus GⅡ 4 Sydney variant-related outbreaks in Korea", Osong Public Health Res Perspect, 6, pp.322~326 (2015)
- Koopmans M, "Foodborne viruses", FEMS Microbiol, 26, pp.187~205 (2002)
- A. Kroneman, "Analysis of Integrated Virological and Epidemiological Reports of Norovirus Outbreaks Collected within the Foodborne Viruses in Europe Network from 1 July 2001 to 30 June 2006", Journal of Clinical Microbiology, 46(9), pp.2959~2965 (2008), American Society for Microbiology[DOI]
- 이태식, "패류 양식장의 세균학적 수질에 미치는 강우의 영향", 한국수산과학회지, 43(5), pp.406~414 (2010), 한국수산과학회
- Lee JE, "Inactivation and UV disinfection of murine norovirus with TiO2 under vatious environmental conditions", Appl Environ Micrbiol, 74, pp.2111~2117 (2008)
- 문애리, "Prevalence of Noroviruses in Oysters in Korea", Food Science and Biotechnology, 20(4), pp.1151~1154 (2011), 한국식품과학회
- 오은경, "패류 중 자연정화 및 인공정화에 의한 위생지표세균의 변화", 한국수산과학회지, 45(1), pp.11~16 (2012), 한국수산과학회
- Elaine Scallan, "Foodborne Illness Acquired in the United States—Major Pathogens", Emerging Infectious Diseases, 17(1), pp.7~15 (2011), Centers for Disease Control and Prevention (CDC)[DOI]
- Seo K, "Effect of temperature, pH and NaCl on the inactivation kinetics of murine norovirus", J Food Protect, 69, pp.2761~2765 (2012)
- 신순범, "Genetic Diversity of Noroviruses Detected in Oysters in Jinhae Bay,Korea", Food Science and Biotechnology, 22(5), pp.1453~1460 (2013), 한국식품과학회[DOI]
- 신순범, "통영시 연안의 양식굴(Crassostrea gigas)에서 검출된 노로바이러스의 정량분석", 한국수산과학회지, 47(5), pp.501~507 (2014), 한국수산과학회
- Simmons G, "A New Zealand outbreak of norovirus gastroenteritis linked to the consumption of imported raw Korean oysters", The New Zealand Medical J, 120, pp.U2773~ (2007)
- Y. Ueki, "Persistence of Caliciviruses in Artificially Contaminated Oysters during Depuration", Applied and Environmental Microbiology, 73(17), pp.5698~5701 (2007), American Society for Microbiology[DOI]
- Webby RJ, "Internationally distributed frozen oyster meat causing multiple outbreaks of norovirus infection in Australia", Clinical Infectious Disease, 44, pp.1026~1031 (2007)