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페놀 화합물의 Hexavalent Chromium독성에 대한 세포 보호효과

Cytoprotective Effect of Phenolic Compounds Against Hexavalent Chromium-Induced Cytotoxicity

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Objectives: In order to evaluate the cytotoxicity of hexavalent chromium, the cytoprotective effect of phenolic compounds against hexavalent chromium-induced cytotoxicity, cell viability, cell adhesion ability, lactate dehydrogenase(LDH) activity, and morphological changes of cells were examined. Methods: We measured the cytotoxicity of hexavalent chromium with 3-[4,5-dimethyl-thiazol-2-yl]-2,5- diphenyltetrazolium bromide(MTT), 2,3-bis-[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-caboxanilide (XTT), LDH and DPPH methods. Results: The cytotoxicity of hexavalent chromium(IC50, 44.0-51.0μM) was high according to the toxic criteria. Cytoprotective effect of phenolic compounds against IC50 value of hexavalent chromium in cell morphology increased in a concentration-dependent manner. Conclusions: These results suggest that 3,4,5-trihydroxybenzoic acid may be used as a cytoprotective agent against chromium(IV)-mediated cytotoxicity.

Abstract

Objectives: In order to evaluate the cytotoxicity of hexavalent chromium, the cytoprotective effect of phenolic compounds against hexavalent chromium-induced cytotoxicity, cell viability, cell adhesion ability, lactate dehydrogenase(LDH) activity, and morphological changes of cells were examined. Methods: We measured the cytotoxicity of hexavalent chromium with 3-[4,5-dimethyl-thiazol-2-yl]-2,5- diphenyltetrazolium bromide(MTT), 2,3-bis-[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-caboxanilide (XTT), LDH and DPPH methods. Results: The cytotoxicity of hexavalent chromium(IC50, 44.0-51.0μM) was high according to the toxic criteria. Cytoprotective effect of phenolic compounds against IC50 value of hexavalent chromium in cell morphology increased in a concentration-dependent manner. Conclusions: These results suggest that 3,4,5-trihydroxybenzoic acid may be used as a cytoprotective agent against chromium(IV)-mediated cytotoxicity.

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