페놀산의 수산기 수와 생리활성과의 관계에 관한 연구
Studies on the Relationship between Hydroxyl Radical Number and Biological Activity of Phenolic Acid
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In order to find selective substances which have strong toxic effect on cancer cell line and non-toxic effect on nonnal cell line by the biological activity according to the nurnber of hydroxy radicals conjugated aromatic ring of phenolic acid, N퍼3T3 fibroblasts and hurnan oral epithelioid carcinoma cells were treated with 3-HBA (3-hydroxybenzoic acid) having aromatic ring conjugated one hydroxy radical, 2,3-DHBA (2,3-dihydroxybenzoic acid) having two hydroxy radical or 3,4,5-THBA (3,4,5-trihydroxybenzoic acid, gallic acid) having three hydroxy radical. Cytotoxicity was determined by MTT assay for cell viability and XTT assay for cell adhesion ability. And also, DPPH radical scavenging activiη was perfonned to examine the antioxidant activity in these cell lines. The results were as follows : l. 3-HBA, 2,3-DHBA and 3,4,5-THBA decreased cell viability and cell adhesion ability on NIH3T3 fibroblasts in dose-dependent manner, but they did not show statistically significance in cell viability and cell adhesion ability compared with control. 2. 3-HBA, 2,3-DHBA and 3,4,5-THBA decreased cell viability and cell adhesion ability dose dependently on human oral epitheloid carcinoma cells, and also, they showed significant decrease at 50 ‘lM(P<O.05) and 100 ‘lM( P<O.OI) in these cells. 3. The range of ICso by MTT assay and XTT assay in human oral epithelioid carcinoma cells was 3-HBA > 2,3-DHBA > 3,4,5-THBA. 4. 3-HBA, 2,3-DHBA and 3,4,5-THBA increased DPPH radical scavenging activity in dose-dependent manner, and also, the radical scavenging activity of 2,3• DHBA and 3,4,5-THBA which had more hydroxy radical were more stronger than that of 3-HBA. From these results, it is suggested that the more nurnber of hydroxy radicals conjugated aromatic ring of phenolic acid increased, the more strong, the biological activity.
Abstract
In order to find selective substances which have strong toxic effect on cancer cell line and non-toxic effect on nonnal cell line by the biological activity according to the nurnber of hydroxy radicals conjugated aromatic ring of phenolic acid, N퍼3T3 fibroblasts and hurnan oral epithelioid carcinoma cells were treated with 3-HBA (3-hydroxybenzoic acid) having aromatic ring conjugated one hydroxy radical, 2,3-DHBA (2,3-dihydroxybenzoic acid) having two hydroxy radical or 3,4,5-THBA (3,4,5-trihydroxybenzoic acid, gallic acid) having three hydroxy radical. Cytotoxicity was determined by MTT assay for cell viability and XTT assay for cell adhesion ability. And also, DPPH radical scavenging activiη was perfonned to examine the antioxidant activity in these cell lines. The results were as follows : l. 3-HBA, 2,3-DHBA and 3,4,5-THBA decreased cell viability and cell adhesion ability on NIH3T3 fibroblasts in dose-dependent manner, but they did not show statistically significance in cell viability and cell adhesion ability compared with control. 2. 3-HBA, 2,3-DHBA and 3,4,5-THBA decreased cell viability and cell adhesion ability dose dependently on human oral epitheloid carcinoma cells, and also, they showed significant decrease at 50 ‘lM(P<O.05) and 100 ‘lM( P<O.OI) in these cells. 3. The range of ICso by MTT assay and XTT assay in human oral epithelioid carcinoma cells was 3-HBA > 2,3-DHBA > 3,4,5-THBA. 4. 3-HBA, 2,3-DHBA and 3,4,5-THBA increased DPPH radical scavenging activity in dose-dependent manner, and also, the radical scavenging activity of 2,3• DHBA and 3,4,5-THBA which had more hydroxy radical were more stronger than that of 3-HBA. From these results, it is suggested that the more nurnber of hydroxy radicals conjugated aromatic ring of phenolic acid increased, the more strong, the biological activity.