Chromium Trioxide의 독성에 대한 산수유 추출물의 항산화 효과
Antioxidative Effects of Cornis fructus Extract on Chromium Trioxide Toxicity
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초록
본 연구 목적은 산수유 추출물의 항산화 효과를 배양 C6 glioma 세포를 대상으로 조사하였다. 이 같은 목적을 위하여C6 glioma 세포를 48시간 동안 배양한 후 6가 크롬의 세포독성및 산수유 추출물의 방어효과를 조사하였다. 이외에 EDA 및LDH 활성과 같은 항산화 효과를 분석하였다. 본 연구에서CrO3는 처리농도에 비례하여 세포생존율을 감소시켰다. 또한이 과정에서 세포를 48시간 동안 20∼40 uM로 각각 포함된 배양액에서 처리한 결과 XTT50 값은 33 uM로 나타났다. 항산화제인 CAT는 CrO3로 유도된 세포독성에 의해 감소된 세포생존율을 유의하게 증가시켰다. 한편 산수유 추출물의 보호효과에있어서, 산수유 추출물은 CrO3만의 처리군에 비하여 세포생존율을 유의하게 증가시켰다. 동시에 산수유 추출물은 EDA와LDH 활성과 같은 항산화 효과를 보였다. 이와 같은 결과로부터CrO3의 독성에 산화적 손상이 관련되어 있는 것으로 나타났다. 또한 산수유 추출물은 이의 항산화 효과에 의하여 CrO3의 세포독성을 효과적으로 방어하였다. 결론적으로, 산수유 추출물과같은 천연소재는 산화적 손상과 관련된 중금속에 의해 유발된세포독성을 방어 내지는 치료하는데 유용한 치료적 요소의 하나로 생각된다.
Abstract
This study examined the antioxidative effects of Cornis fructus (CF) extract on cultured C6 glioma cells. For this purpose, cytotoxicity analysis of chromium trioxide (CrO3) was performed and the protective effects of the CF extract on CrO3–induced cytotoxicity was examined after the C6 glioma cells were cultured for 48 hours. The antioxidative effects, such as electron donating activity (EDA) and lactate dehydrogenase (LDH) activity were also analyzed. In this study, CrO3 decreased the cell viability in a dose dependent manner. The XTT50 value was determined to be 33 M after the cells were treated for 48 hours at a concentrations of 20∼40 M CrO3. The catalase (CAT) antioxidant increased significantly the cell viability that had been decreased by CrO3–induced cytotoxicity. Regarding the protective effect of the CF extract, the cell viability of the CF extract was increased significantly compared to that of CrO3 only. In addition, the CF extract showed antioxidative effects, such as EDA and an inhibitory effect on the LDH activity. These findings suggest that the cytotoxicity of CrO3 is correlated with oxidative stress, and the CF extract effectively prevented CrO3–induced cytotoxicity through the antioxidative effects. In conclusion, natural products, such as the CF extract may be a useful therapeutic agent for the prevention or treatment of toxicity induced by heavy metals via oxidative stress.
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