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3,4,5-Trihydroxybenzoic Acid와 관련 화합물의 구강암 세포주에 대한 세포독성효과

The Cytotoxic Effect of 3,4,5-Trihydroxybenzoic Acid and Related Compounds against Oral Cancer Cell Lines

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Objectives:In order to evaluate the cytotoxicity of 3,4,5-trihydroxybenzoic acid and related compounds on the growth of normal cell lines and human oral epithelioid cell line, cell viability, cell adhesion ability, and morphological changes of cells were examined. Methods:We measured the cytotoxicity of 3,4,5-trihydroxybenzoic acid and related compounds with 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide-[MTT), and 2,3-bis-[2-methoxy-4-nitro- 5-sulfophenyl]-2H-tetrazolium -5-caboxanilide-[XTT) methods. Results:The cytotoxicity of 3,4,5-trihydroxybenzoic acid(IC50, 2,552.40 μM) was low according to the toxic criteria. Cytotoxic effect of 3,4,5-trihydroxybenzoic acid and related compounds against IC50 value in cell morphology increased in a concentration-dependent manner. In light microscopy, 100 μM 3,4,5- trihydroxy-benzoic acid showed th highest cytotoxic activity. Conclusions:These results suggest that 3,4,5-trihydroxybenzoic acid may have a potential anticancer activity.

Abstract

Objectives:In order to evaluate the cytotoxicity of 3,4,5-trihydroxybenzoic acid and related compounds on the growth of normal cell lines and human oral epithelioid cell line, cell viability, cell adhesion ability, and morphological changes of cells were examined. Methods:We measured the cytotoxicity of 3,4,5-trihydroxybenzoic acid and related compounds with 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide-[MTT), and 2,3-bis-[2-methoxy-4-nitro- 5-sulfophenyl]-2H-tetrazolium -5-caboxanilide-[XTT) methods. Results:The cytotoxicity of 3,4,5-trihydroxybenzoic acid(IC50, 2,552.40 μM) was low according to the toxic criteria. Cytotoxic effect of 3,4,5-trihydroxybenzoic acid and related compounds against IC50 value in cell morphology increased in a concentration-dependent manner. In light microscopy, 100 μM 3,4,5- trihydroxy-benzoic acid showed th highest cytotoxic activity. Conclusions:These results suggest that 3,4,5-trihydroxybenzoic acid may have a potential anticancer activity.

참고문헌 (31)

  1. Faber E, "The multistep nature of cancer development", Cancer Res., 44, pp.4217~4223 (1984)
  2. Weinstein IB, "The origin of human cancer;molecular mechanisms of carcinogenesis and their implications for cancer prevention and treatment", Cancer Res., 48, pp.4135~4139 (1998)
  3. Schlepper JR, "Prevention, detection and diganosis of head and neck cancer", Semi Oncol, 5, pp.139~149 (1989)
  4. Woods KV, "Analysis of human papilloma virus DNA in oral squamous cell carcinomas", J Oral Pathol Med., 2, pp.101~108 (1993)
  5. Slaughter DL, "Field cacerization in oral stratified squamous epithelium. clinical implication of muticentric origin", Cancer, 6, pp.963~968 (1953)
  6. Shin DM, "Activation of p53 gene expression in premalignant lesions during head and neck tumorigenesis", Cancer Res., 54, pp.321~326 (1994)
  7. Voravud N, "Detection of chromosomes 7 and 17 aneuploidies during multistep carcinogenesis of head and neck cancer by in sit hybridization", Cancer Res, 53(3), pp.2874~2883 (1993)
  8. Ray D, "Differential effects of tri-n-butylstannyl benzoates on induction of apoptosis in K562 and MCF-7 cells", IUBMB Life, 49, pp.519~525 (2000)
  9. Lee HS, "Tyrosinase inhibitors of Pulsatilla cernua root-derived materials", J Agric Food Chem., 50, pp.1400~1403 (2002)
  10. Ishiwata H, "Estimation of preservative concentrations in foods and their daily intake based on official inspection results in Japan in fiscal year 1998", Shokuhin Eiseigaku Zasshi, 42, pp.404~412 (2001)
  11. Reichelt M, "Benzoic acid glucosinolate esters and other glucosinolates from Arabidopsis thaliana", Phytochem, 59, pp.663~671 (2002)
  12. Wu TS, "The constituents of the root and stem of Aristolochia cucurbitifolia Hayata and their biological activity", Chem Pharm Bull, 48, pp.1006~1009 (2000)
  13. Kammerer D, "Characteriztion of phenolic acids in black carrots(Daucus carota ssp. sativus var. atrorubens Alef.) by high-performance liquid chromatography ionization mass spectrometry", Rapid Commun Spectrom, 18, pp.1331~1340 (2004)
  14. Abd El-Mawla AA, "Benzoic acid biosynthesis in cell cultures of Hypericum androsaemum", Planta, 214, pp.727~733 (2002)
  15. Wilhelm KP, "Quantitative assessment of primary skin irritants in vitro in a cytotoxicity model: comparison with in vivo human irritation tests", Br J Dermatol, 145, pp.709~715 (2001)
  16. Nunez Selles AJ, "Isolation and quantitative analysis of phenolic antioxidants, free sugars, and polyols from mango(Mangifera indica L.) stem bark aqueous decoction used in Cuba as a nutritional supplement", J Agric Food Chem., 50, pp.762~766 (2002)
  17. benzoic acid(TAC-101) inhibits the intrahepatic spread of hepatocellular carcinoma and prolongs the life-span of tumor-bearing animals, "Clin Exp Metastasis", 1998, 633~643
  18. De Graff WG, "Protection against adriamycin cytotoxicity and inhibition of DNA topoisomerase Ⅱ activity by 3,4-dihydroxybenzoic acid", Int J Oncol., 23, pp.159~163 (2003)
  19. "2003", 58
  20. Marom M, "Selective inhibition of ras-dependent cell growth by farnesylthiosalisylic acid", J Biol Chem., 270, pp.22263~22270 (1995)
  21. Abu-Amsha R, "Phenolic content of various beverages determines the extent of inhibition of human serum and low-density lipoprotein oxidation in vitro: Identification and mechanism of action of some cinnamic acid derivatives from red wine", Clin Sci., 91, pp.449~458 (1996)
  22. Rice-Evans CA, "Structure-antioxidant activity relationships of flavonoids and phenolic acids", Free Radic Biol Med., 20, pp.933~956 (1996)
  23. 한두석, "페놀 화합물의 Hexavalent Chromium독성에 대한 세포 보호효과", 대한예방한의학회지, 13(2), pp.1~18 (2009), 대한예방한의학회
  24. Sauvant MP, "Effects of humic substances and phenolic compounds on the in vitro toxicity of aluminium", Ecotoxicol Environ Saf., 44, pp.47~55 (1999)
  25. Mosmann T, "Rapid colorimetric assays for cellular growth and survival: Application to proliferation and cytotoxicity assays", J Immunol Methods, 65, pp.55~63 (1983)
  26. 채영암, "기초생물통계학 제 9장 직선회귀", pp.179~198 (1991), 학문사
  27. Valgimigli M, "HydroxcochidicaiVAeneration, levels of tumor necrosis factor-aipha, and progression to heart failure after acute myocardial in fmigtion", J Am Coll Cardiol., 43, pp.l000~l008 (2004)
  28. Kalenilova EI, "Role of reactive oxygen species in the sensitivity of rat hypertrophied myocardium to ischemia", Biochem., 69, pp.311~316 (2004)
  29. Hashimoto T, "Selective brain hypothermia protects against hypoxic-ischemic injury in newborn rats by reducing hydroxyl radical production", Kobe J Med Sci., 49, pp.83~91 (2003)
  30. 한두석, "벤조산과 관련 화합물의 구강암 세포주에 대한 세포독성효과", 약 학 회 지, 52(4), pp.252~258 (2008), 대한약학회
  31. Borenfreund E, "Comparisons of two in vitro cytotoxicity assay. The neutral red(NR) and tetrazolium MTT tests", Toxicol In Vitro, 2, pp.1~8 (1988)

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