Chromium Trioxide로 유발된 세포손상에 대한 Lavender 오일의 항산화 효과
Antioxidative Effect of Lavender Oil on Chromium Trioxide-Induced Cell Injury
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The cell viability by XTT assay, DPPH-radical scavenging activity, superoxide dismutase (SOD)-like activity and lipid peroxidation were measured to evaluate the cell injury of chromium trioxide (CrO_3) and the antioxidative effect of lavender (Lavendula angustifolia L.) oil on the cell injury induced by CrO_3 in cultured C6 glioma cells. C6 glioma cells were then treated with the media containing 30~50 μM of CrO_3 for 48 hours. The results of this study showed that CrO_3 remarkably decreased cell viability in dose-dependent manner and the XTT_50 value was appeared at 40.5 μM of CrO_3. In the effect of antioxidant, SOD on CrO_3-mediated cytotoxicity significantly increased the cell viability which was decreased by CrO_3. Meanwhile, in the protective effect of lavender oil on the cytotoxicity induced by CrO_3, it remarkably increased the cell viability damaged by CrO_3-mediated cell injury and also showed the DPPH-radical scavenging activity, SOD-like activity and the inhibitory activity of lipid peroxidation. From these results, it is revealed that the cell injury by CrO_3 was involved in oxidative stress and lavender oil was effective in the protection of CrO_3-mediated cell injury by antioxidative effect.
Abstract
The cell viability by XTT assay, DPPH-radical scavenging activity, superoxide dismutase (SOD)-like activity and lipid peroxidation were measured to evaluate the cell injury of chromium trioxide (CrO_3) and the antioxidative effect of lavender (Lavendula angustifolia L.) oil on the cell injury induced by CrO_3 in cultured C6 glioma cells. C6 glioma cells were then treated with the media containing 30~50 μM of CrO_3 for 48 hours. The results of this study showed that CrO_3 remarkably decreased cell viability in dose-dependent manner and the XTT_50 value was appeared at 40.5 μM of CrO_3. In the effect of antioxidant, SOD on CrO_3-mediated cytotoxicity significantly increased the cell viability which was decreased by CrO_3. Meanwhile, in the protective effect of lavender oil on the cytotoxicity induced by CrO_3, it remarkably increased the cell viability damaged by CrO_3-mediated cell injury and also showed the DPPH-radical scavenging activity, SOD-like activity and the inhibitory activity of lipid peroxidation. From these results, it is revealed that the cell injury by CrO_3 was involved in oxidative stress and lavender oil was effective in the protection of CrO_3-mediated cell injury by antioxidative effect.
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