Saccharomyces boulardii 세포벽 추출물이 in vitro 반추위 발효성상에 미치는 영향
Effects of Cell Wall Extracts from Saccharomyces boulardii on in vitro Rumen Fermentation
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본 연구의 목표는 S. boulardii 유래 세포벽 추출물(CWSB)의 첨가가 반추위 발효에 미치는 영향을 평가하는 것이다. 본 연구의 in vitro 발효 실험은 CWSB의 첨가용량이 다른 2개의 실험으로 구성되었다. 실험 1에서는 대조구(CON, 시험사료)와 CWSB0.05(시험사료+CWSB 0.5g/kg 첨가), CWSB0.075(시험사료+CWSB 0.75g/kg 첨가)의 처리구로 구성되었고, 실험 2의 처리구는 대조구(CON, 시험사료)와 CWSB0.5(시험사료+ CWSB 5g/kg 첨가), CWSB1.0(시험사료+CWSB 10g/kg 첨가)의 처리구로 구성되었다. In vitro 배양 이후 3, 6, 12, 24, 48h 동안 발생한 가스 발생량 및 48h 배양 이후 발효 성상을 분석하였다. 실험 1에서는 CWSB0.05 처리구에서 유의적으로 높았던 Vmax(p<0.01)를 제외한 모든 반추위 발효성상에서 처리구간 유의적 차이가 관찰되지 않았고, 이에 따라 사료 내 0.5%, 1%의 고용량 첨가 수준을 설정한 실험 2를 수행하였다. 실험 2에서의 CWSB1.0 처리구는 대조구에 비해 총 VFA 발생량이 유의적으로 높았으나 전체 배양시간 내 가스 발생량 및 건물 소화율, 섬유소 소화율은 대조구와 유의적인 차이를 나타내지 않았다. 본 연구 결과를 종합해 볼 때, CWSB의 첨가수준은 0.5% 이상에서 반추위 발효 개선효과를 나타낼 것으로 추정된다.
Abstract
The aim of this study was to investigate the effects of dietary supplementation with yeast cell wall extracts from Saccharomyces boulardii (CWSB) on rumen fermentation in an in vitro model. Experiment 1 comprised control (CON, no additive), CWSB0.05 (0.5 g CWSB/kg of substrate), and CWSB0.075 (0.75 g CWSB/kg of substrate) treatment groups, and experiment 2 comprised control (CON, no additive), CWSB0.5 (5 g CWSB/kg of substrate), and CWSB1.0 (10 g CWSB/kg of substrate) treatment groups. In vitro fermentation experiments were performed for 48 h. Gas production was measured after 3, 6, 12, 24, and 48 h of incubation. At the end of the incubation period, in vitro dry matter degradability (IVDMD), neutral detergent fiber degradability (IVNDFD), pH, volatile fatty acid (VFA) levels, and ammonia nitrogen (NH3-N) levels were evaluated. In experiment 1, no significant differences in gas production were observed at any incubation time points, but the theoretical maximum gas production (Vmax) was significantly higher in the CWSB0.05 treatment group (p<0.01) than the other treatment groups. Fermentation parameters, including nutrient degradability, pH, and VFA and NH3-N levels, did not differ between the treatment groups (p>0.05). In experiment 2, gas production after 12 h of incubation tended to increase after supplementation with 1% CWSB (p=0.09). The CWSB1.0 group showed an increased total VFA production, but there were no differences in IVDMD or IVNDFD among the treatment groups. The results of the present study confirmed that a supplementation rate greater than 0.5% CWSB might be recommended to improve rumen fermentation.
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