Anavrin®의 수준별 첨가가 In vitro 반추위 발효 및 메탄 생성에 미치는 영향
Effects of Different Supplemental Levels of Anavrin® on In vitro Rumen Fermentation and Methane Production
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This study aimed to evaluate the effects of Anavrin® supplementation on ruminal in vitro fermentation characteristics and methane production. Anavrin® was supplemented at levels of 0.00%, 0.03%, 0.05%, 0.10%, 0.50%, and 1.00%, and the fermentation was analyzed after 6 and 24 hours of incubation. At the levels of 0.03% and 0.05%, methane production was decreased by 21-27% compared to the control without alteration of the ruminal fermentation. However, in the groups with supplementation levels greater than 0.10%, reductions in total volatile fatty acid (VFA) production were observed, indicating potential disruptions in microbial population changes. The optimal supplementation level of Anavrin® was determined to be 0.037% using a linear plateau model. The optimization was targeted by achieving a balance between methane reduction and total VFA production. In conclusion, Anavrin® shows potential as a sustainable feed additive for reducing methane emissions in ruminants. However, as the current findings are based on in vitro conditions, further in vivo studies are necessary to validate its effectiveness and determine its practical applications.
Abstract
This study aimed to evaluate the effects of Anavrin® supplementation on ruminal in vitro fermentation characteristics and methane production. Anavrin® was supplemented at levels of 0.00%, 0.03%, 0.05%, 0.10%, 0.50%, and 1.00%, and the fermentation was analyzed after 6 and 24 hours of incubation. At the levels of 0.03% and 0.05%, methane production was decreased by 21-27% compared to the control without alteration of the ruminal fermentation. However, in the groups with supplementation levels greater than 0.10%, reductions in total volatile fatty acid (VFA) production were observed, indicating potential disruptions in microbial population changes. The optimal supplementation level of Anavrin® was determined to be 0.037% using a linear plateau model. The optimization was targeted by achieving a balance between methane reduction and total VFA production. In conclusion, Anavrin® shows potential as a sustainable feed additive for reducing methane emissions in ruminants. However, as the current findings are based on in vitro conditions, further in vivo studies are necessary to validate its effectiveness and determine its practical applications.