항생제 대체제로서 Dicarboxylic Acid 급여가 in vitro 반추위 발효성상, 착유우의 유량 및 유성분에 미치는 영향
Effects of Dicarboxylic Acid as an Alternative to Antibiotic on in vitro Rumen Parameters, Milk yield and Milk Compositions in Lactating Cows
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
This study was undertaken to investigate the effects of dicarboxylic acid supplementation, as replacement antibiotics, of on in vitro ruminal parameters and milk yield and milk composition in lactating cows. in vitro treatments were 1) Con (4 g of basal diet), 2) CM (4 g of basal diet + 0.05 ml of monensin), 3) CR (4 g of basal diet+0.1 ml of dicarboxylic acid) and in vivo treatments were 1) Con (25 kg of basal diet/head/day), and 2) CR (25 kg of basal diet+5 g of dicarboxylic acid/head/day), respectively. A total 10 lactating dairy cows (649±19 kg average body weight, 99±65 average milking days) were divided in to two groups according to mean milk yield and number of days of postpartum. The cows fed a basal diet during adaptation (2 wk) and experimental diets during the treatment periods (4 wk). In the first in vitro experiment, there were no statistical differences between treatments in pH, gas production, and ammonia-N and lactic acid concentration during incubation. However, dry matter digestibility was significantly higher in CR treatment compared to control or CM treatment (P<0.05). Total VFA was tended to higher in CR treatment than those of control and CM treatment (P> 0.05). In the second experiment, milk yield was significantly higher in treatment (40.39 kg) compared to control (35.19 kg), (P<0.05). Milk composition and MUN were not changed by dietary supplementing dicarboxylic acid. Therefore the present results reporting that supplementation of dicarboxylic acid might enhance the stabilization of ruminal fermentation and increase the milk yield of lactating cows.
Abstract
This study was undertaken to investigate the effects of dicarboxylic acid supplementation, as replacement antibiotics, of on in vitro ruminal parameters and milk yield and milk composition in lactating cows. in vitro treatments were 1) Con (4 g of basal diet), 2) CM (4 g of basal diet + 0.05 ml of monensin), 3) CR (4 g of basal diet+0.1 ml of dicarboxylic acid) and in vivo treatments were 1) Con (25 kg of basal diet/head/day), and 2) CR (25 kg of basal diet+5 g of dicarboxylic acid/head/day), respectively. A total 10 lactating dairy cows (649±19 kg average body weight, 99±65 average milking days) were divided in to two groups according to mean milk yield and number of days of postpartum. The cows fed a basal diet during adaptation (2 wk) and experimental diets during the treatment periods (4 wk). In the first in vitro experiment, there were no statistical differences between treatments in pH, gas production, and ammonia-N and lactic acid concentration during incubation. However, dry matter digestibility was significantly higher in CR treatment compared to control or CM treatment (P<0.05). Total VFA was tended to higher in CR treatment than those of control and CM treatment (P> 0.05). In the second experiment, milk yield was significantly higher in treatment (40.39 kg) compared to control (35.19 kg), (P<0.05). Milk composition and MUN were not changed by dietary supplementing dicarboxylic acid. Therefore the present results reporting that supplementation of dicarboxylic acid might enhance the stabilization of ruminal fermentation and increase the milk yield of lactating cows.
참고문헌 (27)
- A. O. A. C., "Official Methods of Analysis" (1990), Association of Official Agricultural Chemists
- Callaway, T. R., "Effect of organic acid and monensin treatment on in vitro mixed ruminal microorganisms fermentation of cracked corn", J. Anim. Sci, 74, pp.1982~1989 (1996)
- Chaney, A. L., "Modified reagent for determination of urea and ammonia", Clinical Chemistry, 8, pp.130~132 (1962)
- Relation between percentage fat content and yield of milk. Correction of milk yield for fat content, "Gaines, W. L.", Illinois Agr. Expt. Sta, ~, pp.Bull. No. 245 (1923)
- Goering, H. K., "Agric. Handbook 379" (1970), ARS
- Higginbotham, G. E., "Effect of yeast culture and aspergillus oryzae extract on milk yield in a commercial dairy herd", J. Dairy Sci, 77, pp.343~348 (1994)
- James, E. N., "Bovine acidosis: Implication on laminitis", J. Dairy Sci, 80, pp.1005~1028 (1997)
- Lehninger, A. L., "Biochemistry" (1975), Worth Publishers
- Looper, M. L., "Managing milk composition: evaluating herd potential", New Mexico State University Extension Service. Guide D-104, 1~4 (2001)
- Martin, C., "Influence of sampling site on concentrations and carbohydrate degrading enzyme activity of protozoa and bacteria in the rumen", J. Anim. Sci, 77, pp.979~987 (1999)
- Martin, S. A., "Effect of malate on in vitro mixed ruminal microorganisms fermentatio", J. Anim. Sci, 73, pp.2141~2145 (1995)
- McDougall, E. J., "Studies of ruminant saliva. 1. The composition of output of sheep's saliva", Biochem. J, 43, pp.99~109 (1948)
- Moon, S. J., "Interpretation of protein-energy balance of feeding by milk urea nitrogen and protein contents in lactating Holstein cow", Kor. J. Anim. Sci and Tech, 42, pp.499~510 (2000)
- Moore, G. A., "Effect of growth conditions on the Streptococcus bovis phosphopenol pyruvate glucose phosphotransferase system", J. Anim. Sci, 69, pp.4967~4973 (1991)
- Newbold, C. J., "Mode of action of the yeast Saccharomyces cerevisiae as a feed additive for ruminants", Br. J. Nutr, 76, pp.249~261 (1996)
- Nisbet, D. J., "Effect of furmarate, L-malate and Aspergillus oryzae fermentation extract on D-lactate utilization by the rumen Selenomonas ruminantium", Curr. Microbiol, 26, pp.133~136 (1993)
- Nisbet, D. J., "Factors affecting L-lactate utilization by Selenomonas ruminantium", J. Anim. Sci, 72, pp.1355~1361 (1994)
- Owens, F. N., "Acidosis in cattle : a review", J. Anim. Sci, 76, pp.275~286 (1998)
- Russell, J. B., "Effects of various methane inhibitions on the fermentation of amino acids by mixed rumen microorganisms in vitro", J. Anim. Sci, 59, pp.1329~1338 (1984)
- Russell, J. B, "Effect of ionophore on ruminal fermentation", Appl. Environ. Microbiol, 55, pp.1~6 (1989)
- Sanson, D. W., "Growth response and serum constituents of Holstein bulls fed malic acid", Nutr. Rep. Int, 30, pp.1261~1267 (1984)
- SAS, "Statistics, Version 8.0 Edition" (2002), SAS Institute. Inc
- Stone, W. C., "The effect of subclinical rumen acidosis on milk component", Cornell nutrition conference, pp.40~46 (1999), Cornell University
- Stroble, H. J., "Effect of pH and energy spilling on bacterial protein synthesis by carbohydrate-limited cultures of mixed rumen bacteria", J. Dairy Sci, 69, pp.2941~2947 (1986)
- Tilly, J. M. A., "A two stage technique for in vitro digestion of forage crop", J. Brit. Grass. Soc, 18, pp.104~111 (1963)
- Waghorn, G. C., "Gas production and nitrogen digestion by rumen microbes from deer and sheep", New Zealand J. Agri. Res, 36, pp.493~497 (1993)
- Wanapat, M., "Manipulation of rumen fermentation with organic acids supplementation in ruminants raised in the tropics", Pakis. J. Nutr, 6, pp.20~27 (2007)