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Saponin 함유 식물 추출물의 첨가가 반추위 발효성상과 메탄생성에 미치는 영향

Effects of Saponin Contained Plant Extracts on Ruminal Fermentation Characteristics and Methane Production

이상철|이상석|김경훈|오영균|최창원|이강연|이성실|백열창|전체옥|설용주|옥지운
한국축산학회 (Korean Society of Animal Sciences and Technology)|2011.04|한국축산학회지 (Journal of Animal Science and Technology)|제53권 제2호|pp. 147-154

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사포닌SaponinMethaneProtozoa메탄반추위 발효Ruminal Fermentation프로토조아
주제분류축산학

초록

본 연구는 saponin 함유 식물 추출물을 이용하여 반추위 발효성상과 메탄생성에 미치는 영향을 알아보고자 실시하였다. 반추위액은 볏짚과 농후사료를 5:5 비율로 급여한 거세한우 2두의(체중: 803±0.5kg) 반추위 cannula를 통하여 채취하였다. 채취한 반추위액은 buffer와 1:2(V/V)의 비율로 혼합하여 배양액으로 사용하였으며, 15ml 배양액에 옥수수 전분 0.2g를 첨가하고, saponin 함유 식물 추출물 (비누풀, 오가피, 유카, 인삼 및 차나무)을 0.5% 첨가하여 39℃에서 24시간동안 배양하였다. 반추위 발효성상에 있어서 pH 값은 배양 시간동안 모든 처리구가 대조구에 비해 유의적 (p<0.05)으로 낮게 나타났으며, 총 휘발성 지방산의 함량은 배양 12시간부터 모든 처리구에서 유의적 (p<0.05)으로 높았다. Acetate의 농도는 배양 6시간부터 처리구에서 낮았고, propionate의 농도는 높아 역의 관계를 나타내었다. 암모니아의 농도는 최종 발효시간인 24시간에 오가피와 유카추출물 처리구는 대조구와 비슷한 수준이었지만, 다른 처리구는 유의적(p<0.05)으로 낮았다. 반추위 protozoa의 수는 비누풀 추출물 처리구를 제외한 모든 처리구에서 대조구에 비해 유의적(p<0.05)으로 적었다. 총 가스 발생량은 배양 12시간부터 처리구에서 높은 경향이었지만, 메탄 발생량은 오가피와 비누풀 추출물 처리구를 제외한 나머지 처리구에서 낮게 나타났다. 따라서 본 시험 결과를 볼 때 saponin의 첨가에 의해 메탄 발생량이 감소하는 것은 반추위 protozoa의 감소에 의해 나타난 것으로 생각된다.

Abstract

This study was conducted to evaluate the effects of saponin contained plant extracts on in vitro rumen fermentation characteristics and methane production. Ruminal fluid was collected from rumen cannulated Hanwoo steers fed rice straw and concentrate(5:5). Collected rumen fluids, corn starch and buffer including saponin contained plant extracts(ginseng, Ogapi, soapwort, tea plant and yucca; 0.5%/15ml) were incubated at 39^℃ for 24 h. All incubations were repeated five times. Rumen pH in all treatments was lower(p<0.05) compared with that of the control(no addition) during incubation time. The concentration of total VFA in all treatments was higher(p<0.05) than that of the control after 12h incubation. Compared with the control, the concentration of acetate and propionate in all treatments was lower and higher after 6h incubation, respectively. The concentration of NH3-N in all treatments was lower(p<0.05) than that of the control except for Ogapi or yucca extracts supplementation. The number of protozoa in all treatments was significantly(p<0.05) lower than that of the control except for soapwort extract supplementation. The total gas production and methane production in all treatments was higher(p<0.05) and lower(p<0.05) compared with the control, except for ogapi or soapwort extracts supplementation after 12h incubation, respectively. Therefore, reduction in methane production by saponins may could be results from decreased protozoal population without any negative in vitro fermentation .

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