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볏짚 조사료에 대한 효모 배양물 첨가가 반추위 소화율 및 섬유소 분해균의 군락 변화에 미치는 영향

Effects of Yeast Culture Supplementation on Rice Straw Digestibility and Cellulolytic Bacterial Community in the Rumen

성하균
한국축산학회 (Korean Society of Animal Sciences and Technology)|2013.02|한국축산학회지 (Journal of Animal Science and Technology)|제55권 제1호|pp. 41-49

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키워드

볏짚Saccharomyces cerevisiaeF. succinogenesR. flavefaciensR. albus섬유소분해 박테리아한국 재래산양반추위Rice straw digestion
주제분류축산학

초록

본 연구는 효모(Saccharomyces cerevisiae)가 조사료의 반추위 미생물 분해 및 섬유소 분해 박테리아의 군집 변화에 미치는 관계를 검증하고자, 생효모 배양물 첨가 및 급여가 볏짚 소화율에 미치는 영향을 in vitro 및 in situ 실험을 통해 측정하였고, 볏짚 분해시 볏짚 표면에 부착한 섬유소분해 박테리아 군집의 변화를 real-time PCR 방법을 이용하여 비교 분석하였다. 생효모 배양물의 첨가 수준(0.0, 0.2, 0.4, 0.6, 0.8 및 1.0% )에 따른 볏짚의 in vitro 건물 소화율을 비교 하였을 때 첨가 수준이 증가 할수록 소화율도 점진적으로 높아 졌다. 특히, 생효모 배양물의 첨가 수준을 0.6% 이상으로 하였을 때 0.0, 0.2 및 0.4% 첨가보다도 확연한 소화율 증가를 보이기 시작하였다(p<0.05). 또한 효모배양물의 0.6% 첨가를 NaOH 4% 처리 볏짚 및 무처리 볏짚에 적용하여 소화율을 재평가하였을 때 두 가지 볏짚 모두에서 효모배양물 첨가에 의하여 볏짚 소화율이 유의적으로 증가하였다(p<0.05). 생효모 배양물을 한국재래산양에 실제 급여에 의한 볏짚의 in situ 건물소화율을 24 및 48 시간 후에 관측하였을 때 NaOH 무처리 볏짚의 효모배양물 비급여구의 소화율이 가장 낮았고, NaOH 무처리 볏짚의 효모배양물 급여구, 4% NaOH 처리 볏짚의 효모배양물 비급여구, 그리고 4% NaOH 처리 볏짚의 효모배양물 급여구 순으로 소화율이 유의적으로 각각 증가하였다(p<0.05). 이는 NaOH 무처리 볏짚 및 4% NaOH 처리 볏짚 모두에서 생효모 배양물 급여에 의하여 in situ 건물 소화율이 유의적으로 증가함을 보여준 것이다(p<0.05). 그리고 볏짚을 효모배양물의 두 가지 농도로 볏짚을 분무 처리하여 in situ 소화율과 볏짚 표면 부착 섬유소분해 박테리아(F. succiongenes, R. flavefaciens, R. albus)의 군집 변화를 측정하였을 때 효모배양물 처리 농도가 증가함에 따라 소화율도 유의적으로 높게 나타났고(p<0.05), 동시에 이들 박테리아의 볏짚 표면 부착 군집도 효모배양물의 처리 농도 증가에 따라 증가 하였다. F. succiongenes과 R. flavefaciens는 배양 12 및 24시간 모두 처리농도에 따라 군락의 수가 유의적으로 증가하였다(p<0.05). 그리고 R. albus도 배양 12 시간 처리농도에 따라 군락의 수가 유의적으로 증가하였고(p<0.05), 24 시간도 처리농도에 따라 증가 경향을 나타냈다. 따라서 본 연구는 효모 첨가는 조사료 소화율을 증진에 확실히 좋은 영향을 주며, 이것은 조사료 표면 부착 섬유소분해 박테리아의 군락 형성 증가에서 기인함을 보여준다고 사료된다.

Abstract

In vitro and in situ incubation studies were conducted to determine effects of yeast culture supplements(Saccharomyces cerevisiae) on cellulolytic bacterial function and fiber digestion in rice straw. In vitro dry matter digestibility of rice straw gradually increased according to supplemental levels of yeast culture(0.0, 0.2, 0.4, 0.6, 0.8 and 1.0%). Digestibility of rice straw started to increase apparently when yeast culture was added more than 0.6% level(p<0.05). Also, we reconfirmed that in vitro dry matter digestibility was significantly increased by 0.6% of yeast culture addition in 4% NaOH treated and non-treated rice straws(p<0.05). When in situ dry matter digestibility was tested in Korean native goats fed basal diet or experimental diet which contained 1.0% of yeast culture, the yeast culture feeding improved in situ dry matter digestibility in both 4% NaOH treated and non-treated rice straws(p<0.05). In case of real-time PCR monitoring cellulolytic bacterial function, the bacterial population attached on rice straw showed the increasing trends with higher level of yeast culture spraying on rice straw. F. succinogenes and R. flavefaciens were significantly increased in accordance to spraying levels of yeast culture (0.0, 0.1 and 0.3%) at both 12 and 24 hrs of in situ incubation(p<0.05). R. albus was significantly higher population in yeast culture spraying than non-soraying at 12 hrs of in situ incubation(p<0.05). These bacterial populations were showed the increasing trends with digestibility enhancement of rice straw according to the higher levels of yeast culture supplement. Overall, these results clearly suggest that the presence of yeast culture result in noticeable increase of rice straw digestion, which is modulated via good effect on cellulolytic bacterial attachment to fiber substrates.

참고문헌 (56)

  1. Adams, D. C., "Influence of viable yeast culture, sodium bicarbonate and menensin on liquid dilution rate, rumen fermentation and feedlot performance of growing steers and digestibility in lambs", J. Anim. Sci, 53, pp.780~782 (1981)
  2. Chaucheyras-Durand, F, "Establishment of cellulolytic bacteria and development of fermentative activities in the rumen of gnotobiotically-reared lamb receiving the microbial additive Saccharomyces cerevisiae Cncm I-1077", Reprod. Nutr. Dev, 41, pp.57~68
  3. Chaucheyras-Durand, F., "Influence of a probiotic yeast(Sccharomyces cerevisiae CNCM I-1077) on microbial colonization and fermentation in the rumen of newborn lambs", Microb. Ecol. Health Dis, 14, pp.30~36 (2002)
  4. Chevaux, E., "Probiotic yeast in small ruminants", Feed Mix, 15, pp.2809~2016 (2007)
  5. Dann, H. M., "Effests of a yeast culture(Saccharomyces cerevisiae) on prepartum intake and postpartum intake and milk production of Jersey cows", J. Dairy Sci, 83, pp.123~127 (2000)
  6. Dawson, K. A., "Mode of action of yeast cultures in rumen-natural fermentation modifiers", Proceedings, Alltech's Third Annual Symposium. Biotechnology in the Feed Industry. Lexington. USA (1987)
  7. Dawson, K. A., "Current and future role of yeast culture in animal production: A review of research over the last six years", In: Supplement to the Proceedings of Alltech's 8th Annual Symposium. Alltech Technical Publication, Nicholasville, KY, USA, pp.45~56 (1992)
  8. Dawson, K. A., "Current and future role of yeast culture in animal production: A review of research over the last six years", In: Supplement to the proceedings of Alltech's 8th Annual Symposium. Alltech Technical Publication. Nicholasville, KY, USA, pp.45~46 (1992)
  9. Dawson, K. A., "Differential effects of live yeast on the cellulolytic activities of anaerobic ruminal bacteria", J. Anim. Sci, 69(Suppl. 1), pp.531~ (1991)
  10. Dawson, K. A., "The evolution of yeast cultures-20 years of research. In: Navigating from Niche Markets to Mainstream", Proceeding of Alltech's European, Middle Eastern and African Lecture Tour, pp.26~43 (2002)
  11. Dawson, K. A., "Effects of microbial supplements containing yeast and lactobacilli on roughage-fed ruminal microbial activities", J. Anim. Sci, 68, pp.3392~3398 (1990)
  12. Denev, S. A., "Yeast culture in ruminant nutrition", Bulgarian J. of Agricultureal Sci, 13, pp.357~374 (2007)
  13. Devandra, C., "Perspectives in the utilization of untreated rice straw by ruminants in Asia. In: The utilization of fibrous agricultural residue as animal feeds, In School of Agric. and Foresty" (1982), Univ. of Melvourne
  14. Duncan, D. B., "Multiple range and multiple F test", Biometrics, 11, pp.1~42 (1955)
  15. Fallon, R. J., "Effects of Yea-SaccⓇ 1026 inclusion on the performance of finishing bulls offered an all concentrate diet", Proceeding of the 20th Annual Symposium “Nutritional Biotechnology in the Feed and Food Industries”, (Suppl. 1), pp.75~
  16. Girard, I. D., "Characterization of stimulatory activities of Saccharomyces cerevisiae 1026 on the growth and metabolism of ruminal bacteria", In: Alltech’s 13th Annual symposium Biotechnology in the Feed Industry, Lexington, kentucky, USA, pp.45~47
  17. Gunter, K. D, "Yeast cultures success under German dairy conditions. In: Biotechnology in the Feed Industry", pp.38~ (1989), Alltech's Technical Publications
  18. Harrison, G. A., "Influence of addition of yeast culture supplement to diets of lactating cows on ruminal fermentation and microbial populations", J. Dairy Sci, 71, pp.2967~2979 (1988)
  19. Hoyos, G., "Effects of feeding viable microbial feed additives on performance of lactating cows in a large dairy herd", J. Dairy Sci, 70, pp.217~ (1987)
  20. Jankson, M. G., "The alkali treatment of straw", Anim. Feed Sci. Technol, 2, pp.105~116 (1977)
  21. Jouany, J. P., "Methods of manipulating the microbial metabolism in the rumen", Ann. Zootech, 43, pp.49~62 (1994)
  22. Jouany, J. P., "Manipulation of the rumen ecosystem to support high- performance beef cattle-review", Asian Aus. J. Anim. Sci, 13, pp.96~114 (2000)
  23. Jounany, J. P, "20 years of research and now more relevant than ever- the coming of age of yeast cultures in ruminant diets. In: Respomding to a Changing Agricultural Landscape", Alltech's European, Middle Eastern an African Lecture Tour, 44~69 (2001)
  24. Koike, S., "Development and use of competitive PCR asays for the rumen cellulolytic bacteria : Fibrobacter succinogens, Ruminococcus albus and Ruminococcus flavefaciens", FEMS Microbiol. Lett, 204, pp.361~366 (2001)
  25. Koike, S., "Kinestics of in sacco fiber-attachment of representative ruminal cellulolytic bacteria monitored by competitive PCR", J. Dairy Sci, 86, pp.1429~1435 (2003)
  26. Kumar, U., "Effect of yeast culture supplement on ruminal microbial population and metabolism in buffalo calves fed a high roughage diet", J. Dci. Food Agric, 73, pp.231~236 (1997)
  27. Lee, S. S., "Effects of yeast culture supplements on growth and carcass traits in Korean cattle", Korean J. Anim. Sci, 39, pp.391~400 (1997)
  28. Lynch, H. A, "Effects of Saccharomyces cerevisiae culture and Saccharomyces cerevisiae live cells on in vitro mixed ruminal microorganism fermentation", J. Dairy Sci, 85, pp.2009~2014 (2002)
  29. Martin, S. A., "Influence of a commercial yeast supplement on the in vitro ruminal fermentation", Nutr. Rep. Int, 40, pp.395~417 (1989)
  30. Mathieu, F., "The effect of Saccharomyces cerevisiae and Aspergillus oryzae on fermentation in the rumen of faunated and defaunated sheep. protozoal and probiotic interactions", Reprod. Nutr. Dev, 36, pp.271~287 (1996)
  31. McAllister, T. A., "Microbial attachment and feed digestion in the rumen", J. Anim. Sci, 72, pp.3004~3018 (1994)
  32. McDougall, E. I., "Studies on ruminant saliva. 1. The composition and output of sheep’s saliva", Biochem. J, 43, pp.99~109 (1948)
  33. Mehrez, A. A., "A study of the artificial bac technique for determining the digestibility of feeds in the rumen", J. Agr. Sci, 88, pp.645~652 (1977)
  34. Miller-Webster, T., "Influence of yeast culture on ruminal microbial metabolism in continuous culture", J. Dairy Sci, 85, pp.2009~2014 (2002)
  35. Monsoni, P., "Quantification by real-time PCR of cellulolytic bacteria in the rumen of sheep after supplementation of a forage diet with readily fermentable carbohtdrates: effects of a yeast additive", J. of Appl. Microbiol, 103, pp.2676~2685 (2007)
  36. Mpofu, I. D. T., "The potential of yeast and natural fungi for enhancing fibre digestibility of forage and roughages", Anim. Feed Sci. and Tech, 48, pp.39~47 (1994)
  37. Newbold, C. J., "Mode of action of the yeast Saccharomyces cerevisiae as a feed additive for ruminants", Brit. J. Nutr, 76, pp.249~256 (1996)
  38. Newbold, C. J., "Different strains of Saccharomyces cerevisiae differ in their effects on ruminal bacterial numbers in vitro and in sheep", J. Anim. Sci, 73, pp.1811~1818 (1995)
  39. Padel, A. M. A., "Effects of supplemental yeast(Saccharomyces cerevisiae) culture on NDF digestibility and rumen fermentation of foragr sorghum hay in Nubian goat’s kids", J. Agric. and Biol. Sci, 3, pp.133~137 (2007)
  40. Palata, F. P., "effect of a yeast culture(Saccharomyces cerevisiae) on neutral detergent fiber digestion in steers fed oat straw based diets", Anim. Feed Sci. Tech, 49, pp.210~ (1994)
  41. Park, H. S., "Effect of feeding viable yeast and lactobacillus on milk yield and milk fat content", Korean J. Anim. Sci, 38, pp.77~84 (1996)
  42. Paryad, A, "Effect of yeast(Saccharomyces cerevisiae) on apparent digestibility and nitrogen retention of tomato pomace in sheep", Pakistan J. of Nutrition, 8, pp.273~278 (2009)
  43. Phillips, W. A., "The effect of yeast culture on the post stressperformance of feeder calves", Nutr. Rep. Int, 32, pp.287~296 (1985)
  44. Purdy, K. J., "Rapid extraction of DNA and RNA from sediments by novel hydroxyapatite spin-colum method", Appl. Environ. Microbial, 62, pp.3905~3970 (1996)
  45. Rose, A. H., "Rent research on industrially important strains of Saccharomyces cerevisae, In Biology and Activities of Yeasts. The Society for Applied Bacteriology Symposium Series" (1980), Academic Press
  46. STAT softwareⓇ for PC, "SAS", pp.1996, SAS.
  47. Smith, W. A., "Effects of forage type on production of dairy cows supplemented with whole cottonseed, tallow and yeast", J. Daily Sci, 76, pp.205~213 (1993)
  48. Ha Guyn Sung, "Low Ruminal pH Reduces Dietary Fiber Digestion via Reduced Microbial Attachment", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 20(2), pp.200~207 (2007), 아세아·태평양축산학회
  49. 성하균, "조사료의 곰팡이 발생과 곰팡이독소 오염", 한국초지조사료학회지, 30(1), pp.77~88 (2010), 한국초지조사료학회
  50. Tajima, K., "Diet-Dependent shifts in the bacterial population of the rumen revealed with real-time PCR", Appl. Environ. Microb, pp.2766~2744 (2001)
  51. Van Keuren, R. W, "Study of a nylon bag technique for in vitro estimation of forage disappearance rate", J. Anim. Sci, 21, pp.340~345 (1962)
  52. Wallace, R. J, "The mode of action of yeast culture in modifying rumen fermentation", pp.332~338 (1996), In: Proceedings of Alltech's 12th Annual Symposium on Biotechnology in the Feed Industry. Nottingham University Press. Loughborough, Leics. UK
  53. Weidmeier, R. D., "Effects of supplemental Saccharomyces cerevisiae and", or Aspergillus oryzae on rumen fermentation, 1985, ~
  54. Weidmeier, R. D., "Effect of yeast culture and Aspergillus oryzae fermentation extract on ruminal characteristics and nutrient digestibility", J. Dairy Sci, 70, pp.2063~2079 (1987)
  55. Williams, P. E. V., "The effects of novel microorganisms on rumen fermentation and ruminant productivity. In: Recent Advances in Animal Nutrrition 1990" (1990), Butterworths
  56. Williams, P. E. V., "Effects of the inclusion of the yeast culture (Saccharomyces cerevisiae plus growth medium) in the diet of dairy cows on milk yield and forage degradation and fermentation patterns in the rumen of steers", J. Anim. Sci, 69, pp.3016~3025 (1991)

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