Bifidobacterium ruminantium 첨가가 청보리와 티모시 조사료의 in vitro 반추위 내발효성상과 메탄 발생에 미치는 영향
Effects of Supplementation of Bifidobacterium ruminantium on in vitro Ruminal Fermentation and Methane Production
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
This study was conducted to evaluate effects of supplementation of Bifidobacterium ruminantium on in vitro ruminal fermentation and methane production. Ruminal fermentation characteristics of Timothy hay (C1), whole barley (C2), Timothy hay + B. ruminantium (T1) and whole barley + B. ruminantium (T2) were evaluated at 0, 3, 6, 9, 12, 24 and 48 h incubation at 39℃. The amount of B. ruminantium culture added into T1 and T2 was 0.3 ml. The pH values ranged from 5.99 to 6.83 in all the treatments. Concentration of NH3-N of C2 and T2 was higher than C1 and T1 at 48 h (p<0.05). The total gas production of C2 and T2 was higher than C1 and T1 at 9 h (p<0.05). The total methane production of treatments with B. ruminantium was not significantly different at 24 and 48 h (p<0.05). Concentration of lactic acid was significantly different between both substrates (p<0.05). Therefore, B. ruminantium supplementation was determined to be insignificant in the in vitro ruminal fermentation and methane production, while a further study was required to investigate relation to lactic acid production with different forage sources.
Abstract
This study was conducted to evaluate effects of supplementation of Bifidobacterium ruminantium on in vitro ruminal fermentation and methane production. Ruminal fermentation characteristics of Timothy hay (C1), whole barley (C2), Timothy hay + B. ruminantium (T1) and whole barley + B. ruminantium (T2) were evaluated at 0, 3, 6, 9, 12, 24 and 48 h incubation at 39℃. The amount of B. ruminantium culture added into T1 and T2 was 0.3 ml. The pH values ranged from 5.99 to 6.83 in all the treatments. Concentration of NH3-N of C2 and T2 was higher than C1 and T1 at 48 h (p<0.05). The total gas production of C2 and T2 was higher than C1 and T1 at 9 h (p<0.05). The total methane production of treatments with B. ruminantium was not significantly different at 24 and 48 h (p<0.05). Concentration of lactic acid was significantly different between both substrates (p<0.05). Therefore, B. ruminantium supplementation was determined to be insignificant in the in vitro ruminal fermentation and methane production, while a further study was required to investigate relation to lactic acid production with different forage sources.
참고문헌 (23)
- Annison, E. F, "Tracer Studies on Non-protein Nitrogen for Ruminants-Ⅱ", pp.1975, FAO
- A.O.A.C, "Official Methods of Analysis" (1990), Association of Official Analytical Chemists
- Beuvink, J. M., "An automated method of measuring the time course of gas production of feedstuffs incubated with buffered rumen fluid", Neth. J. Agri. Sci, 40, pp.401~407 (1992)
- Biavati, B., "Bifidobacterium ruminantium sp. Nov. and Bifidobacterium merycicum sp. Nov. from the rumen of cattle", Int. J. Syst, 41, pp.163~168 (1991)
- Chaney, A. L, "Modification reagents for determination of urea and ammonia", Clinical Chem, 8, pp.130~132 (1962)
- Duncan, D. B., "Multiple range and multiple F tests", Biometrics, 11, pp.1~42 (1955)
- Ferorak, P. M., "A simple apparatus for measuring gas production by methanogenic culture in serum bottle", Environ. Technol. Lett, 4, pp.268~ (1983)
- Gray, F. V., "Fermentation in the rumen of the sheep", J. Exp. Biol, 28, pp.83~90 (1951)
- Havenaar, R., "The Lactic Acid Bacteria, Vol. I. The Lactic Acid Bacteria in Health and Disease", pp.1~125 (1992), Elsevier Applied Science Publishers
- Hiltner, P, "Effect of soluble carbohydrates on digestion of cellulose by pure cultures of rumen bacteria", Appl. Environ. Microbiol, 46, pp.642~648 (1983)
- Irmgard Immig, "The rumen and hindgut as source of ruminant methanogenesis", Environ. Monit. Assess, 42, pp.57~72 (1996)
- Maeng, W. J, "Ruminant Nutrition", pp.91~112 (1998), Hyangmunsa
- McDougall, E. I., "Studies on ruminant saliva. I. The composition and output of sheep's saliva", Biochem. J, 43(1), pp.99~109 (1948)
- Mehrez, A. Z., "Rates of rumen fermentation in relation to ammonia concentration", Br. J. Nutri, 38, pp.437~443 (1977)
- Russeil, J. B., "Effect of ionophores on ruminal fermentation", App. Environ. Microbil, 55, pp.1~6 (1989)
- SAS, "SAS procedures guide, Version 8" (1996), SAS Institute Inc
- Satter, L. D., "Effect of ammonia concentration on rumen microbial protein production in-vitro", Br. J. Nutr, 32, pp.199~208 (1974)
- Satter, L. D., "Nitrogen requirement and utilization in dairy cattle", J. Dairy Sci, 58, pp.1219~1237 (1975)
- Scardovi, V, "Bergey’s Manual of Systematic Bacteriology, Vol. 2", pp.1418~1435 (1986), Williams and Wilkins
- Scardovi, V., "Bifidobacteria in bovine rumen", Archiv. Mikrobiol, 68, pp.278~294 (1969)
- Shaw, J. C., "Relation of diet to rumen volatile fatty acids, digestibility, efficiency of gain and degree of unsaturation of body fat in steers", J. Nutr. Sci, 71, pp.203~208 (1960)
- Theodorou, M. K., "A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds", Anim. Feed Sci. Technol, 48, pp.185~197 (1994)
- Van Soest, P. J., "Methods for fiber, neutral detergent fiber, and non starch polysaccharides in relation to animal nutrition", J. Dairy Sci, 74, pp.3583~3597 (1991)