알칸디올의 세포독성 및 항균력의 상관관계와 적용한 제형 안정성에 미치는 영향
Correlation of Cytotoxicity and Antimicrobial Activity of Alkandiols and Their Impact on Stability in Emulsions
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본 연구는 1,2-octanediol과 1,2-hexanediol을 중심으로 ethyl hexanediol, methylpropanediol, 2-butyl-2-ethyl-1,3-propanediol, ethylhexylglycerin의 알칸디올을 유화 에멀젼 제형에 적용하여 미생물 오염에 대한 보존력과 함께 제형 내 상안정성을 확인하여 다양한 화장품 처방 개발에 기여하고자 한다. 방법: 에멀젼에 적용하여 보존력과 3개월 동안 온도 보관에 따른 상안정성을 확인하고 섬유아세포의 세포독성과 균주별 항균력을 비교하였다. 결과: 알칸디올류의 알킬 선형 탄소 사슬 길이가 길수록 보존력이 우수하나 제형 함유 함량이 높을수록 상안정성에 불안정성이 나타났다. 섬유아세포 세포독성 평가를 통해 세포막을 변형시켜 항균력을 나타나는 관계를 확인하였다. 결론: 탄소수와 다이올의 화학구조에 의한 세포독성과 항균력의 상관관계를 활용하여 향 후 미생물 오염에 대한 항균력, 인체에 대한 안전성, 제형의 상안정성 항목에서 상승효과를 부여하는 이상적인 보존제 부스터를 개발하는데 기여할 것으로 기대된다.
Abstract
Purpose: This study aimed to contribute to the development of various cosmetic formulations by adding alkanediols of ethyl hexanediol, methylpropanediol, 2-butyl-2-ethyl-1,3-propanediol, and ethylhexylglycerin (mainly 1,2-octanediol and 1,2-hexanediol) to emulsion formulations to determine their emulsion phase stability as well as their preservation against microbial contamination. Methods: The alkanediols were added to the emulsions to assess their preservation effects and phase stability under various storage temperatures for 3 months. In addition, their fibroblast cytotoxicity and anti-bacterial activity against microorganisms were compared. Results: A greater preservation effect was observed with longer linear alkanediol alkyl carbon chain lengths. However, higher formulation content revealed instability in phase stability. Furthermore, evaluation of fibroblast cytotoxicity showed that the relationship between cell membrane disruption and antimicrobial activity was affected. Conclusion: Utilizing the correlation between cytotoxicity and antimicrobial activity because of the carbon number and diol chemical structure, these results are expected to contribute to the development of improved preservative boosters that provide synergistic effects in terms of antimicrobial activity against microbial contamination, human safety, and the phase stability of formulations in the future.
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