자색 백향과(Passiflora edulis f. edulis) 과피 추출물의 항산화 효과및 세포 생리 활성에 미치는 영향
The Antioxidation Effect of Passiflora edulis f. edulis Rind Extract and Its Influence on Cell Bioactivity
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초록
본 연구를 통해 자색 백향과 과피 추출물이 피부개선을 위한 기능성 화장품의 소재로 활용이 가능성을 확인하고자 항산화활성 및 피부세포에서의 항염, 미백, 항노화 효과를 연구하였다. 실험결과 DPPH, FRAP, TEAC assay 모두 농도 의존적으로 항산화력이 증가하는 양상을 보였으며, 총 폴리페놀의 함량은 105.81 ± 2.85 mg/g이었고, 총 플라보노이드 함량은 15.94 ± 2.61 mg/g으로 나타났다. 세포 내 ROS 저해율에 있어서도 50 μg/mL 농도에서 훌륭한 항산화 효과를 확인하였다. 그리고 피부개선효과를 확인하기 위한 독성실험에서 RAW 264.7, B16F10, CCD-986sk의 모든 세포에서 자색 백향과 과피 추출물을 100 μg/mL 농도까지 처리했을 때 모두 85%이상의 세포 생존율을 확인하였다. RAW 264.7 세포를 이용한 항염 실험에서는 10 μg/mL의 저농도에서도 높은 수준의 NO생성 저해효과가 나타났으며, B16F10 세포를 이용한 미백 실험에서도 10 μg/mL의 저농도에서 멜라닌의 생합성을 효과적으로 억제하였다. CCD-986sk 세포를 이용한 항노화 효능 또한 10 μg/mL의 저농도의 추출물처리에도 MMP-1의 발현량을 현저히 낮췄다. 이와 같은 결과를 종합해 볼 때 자색 백향과 과피 추출물에 함유되어 있는 phenolic compounds의 높은 radical 소거 활성과 세포 내 ROS 생성 억제를 통한 항산화 효과를 나타낸 것으로 보인다. 또한 세포 독성이 낮고, 피부 개선 효과를 가진 화장품 소재로서의 개발 가능성이 있을 뿐만 아니라 주스로 가공하고 남은 자색 백향과의 과피 부분을 재활용하여 사용함으로서 경제적 효율성까지 있을 것으로 판단된다.
Abstract
Currently, the passion fruit (Passiflora edulis f. edulis) used in varying drinks tends to be cultivated more in Korea. The rind of passion fruit is thrown away, and its amount is on the rise. Interestingly, a use for passion fruit rind as a cosmetic product material was recently identified. The rind of passion fruit was extracted in 50% ethanol to measure anti-oxidative activity using DPPH radical elimination analysis, as well as FRAP and TEAC assays. The results of these test reveal high anti-oxidative activity of this extract. In addition, total polyphenol and flavonoid contents were measured. Large amounts of polyphenol and flavonoid influenced anti-oxidative activity. Regarding cell bioactivity, neutral red assay was conducted revealing no cytotoxicity associated with this extract. A degree of the generation suppression of ROS in cells, and anti-inflammatory, skin whitening, and anti-ageing effects were measured. Cell bioactivity was significantly effective at 10 μg/mL (considered a low concentration). Therefore, this study suggests that the extract of passion fruit rind can be used as a functional cosmetic product material.
참고문헌 (45)
- Ahn KS, "Inhibition of inducible nitric oxide synthase and cyclooxygenase II by Platycodon grandiflorum saponins via suppression of nuclear factor-κB activation in RAW 264.7 cells", Life Sci, 76, pp.2315~2328 (2005)
- Ancerewicz, J, "Structure–property relationships of trimetazidine derivatives and model compounds as potential antioxidants", Free Radic Biol Med, 25, pp.113~120 (1998)
- Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Bogdan C, "The role of nitric oxide in innate immunity", Immunol Rev, 173, pp.17~26 (2000)
- Briganti S, "Chemical and instrumental approaches to treat hyperpigmentation", Pigment Cell Res, 16, pp.101~110 (2003)
- 조혜은, "오미자 열수 추출물의 항산화 활성과 아질산 소거능 및 α-Glucosidase 저해 효과", 한국식품영양과학회지, 39(4), pp.481~486 (2010), 한국식품영양과학회
- Choi YJ, "Radical scavenging activities of the extracts from Punica granatum (Pomegrante) Peels", J Natural Sci, 22, pp.111~117 (2010)
- Dhawan K, "Antitussive activity of the methanol extract of Passiflora incarnata leaves", Fitoterapia, 73, pp.397~399 (2002)
- Dhawan K, "Comparative biological activity study on Passiflora incarnata and P. edulis", Fitoterapia, 72, pp.698~702 (2001)
- Duarte J, "Vasodilatory effects of flavonoids in rat aortic smooth muscle. Structure-activity relationships", Gen Pharmacol, 24, pp.857~862 (1993)
- Eruslanov E, "Identification of ROS using oxidized DCFDA and flow-cytometry", pp.57~72 (2010), Humana Press
- Fisher GJ, "Molecular basis of sun-induced premature skin ageing and retinoid antagonism", Nature, 379, pp.335~ (1996)
- Folin O, "On phosphotungstic-phosphomolybdic compounds as color reagents", J Biol Chem, 12, pp.239~243 (1912)
- Guo C, "Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay", Nutr Res, 23, pp.1719~1726 (2003)
- Gutfinger T, "Polyphenols in olive oils", J Am Oil Chem Soc, 58, pp.966~968 (1981)
- Halliwell B, "The characterization of antioxidants", Food Chem Toxicol, 33, pp.601~617 (1995)
- Han SM, "Anti-melanogenic properties of honeybee (Apis mellifera L.) venom in α-MSH-stimulated B16F1 cells", Food Agr Immunol, 26, pp.451~462 (2015)
- Hearing VJ, "Biogenesis of pigment granules: a sensitive way to regulate melanocyte function", J Dermatol Sci, 37, pp.3~14 (2005)
- Heo YS, "Effect of medium composition on in vitro shoot regeneration from leaves of Passiflora species through somatic embryogenesis and callus induction", 2017 Korean J Plant Research Academic Symposium, pp.237~ (2017)
- Hertog MG, "Content of potentially anticarcinogenic flavonoids of tea infusions, wines, and fruit juices", J Agric Food Chem, 41, pp.1242~1246 (1993)
- Hosoi J, "Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid", Cancer Res, 45, pp.1474~1478 (1985)
- Imai J, "Antioxidant and radical scavenging effects of aged garlic extract and its constituents", Planta Med, 60, pp.417~420 (1994)
- Jung CH, "Eleutherococcus senticosus extract attenuates LPS-induced iNOS expression through the inhibition of Akt and JNK pathways in murine macrophage", J Ethnopharmacol, 113, pp.183~187 (2007)
- Kalt W, "Recent research on polyphenolics in vision and eye health", J Agr Food Chem, 58, pp.4001~4007 (2010)
- 강명화, "각 부위별 활나물(Crotalaria sessiflora L.) 에탄올 추출물의 항산화효과", 한국식품과학회지, 34(6), pp.1098~1102 (2002), 한국식품과학회
- Kang S, "Topical N-acetyl cysteine and genistein prevent ultraviolet-light-induced signaling that leads to photoaging in human skin in vivo", J Invest Dermatol, 120, pp.835~841 (2003)
- Kim J, "Inhibition effect of Gynura procumbens extract on UV-B-induced matrix-metalloproteinase expression in human dermal fibroblasts", J Ethnopharmacol, 137, pp.427~433 (2011)
- 이민영, "과일 껍질의 비타민 C , 폴리페놀, 플라보노이드 함량과 항산화 활성", 한국식품과학회지, 44(5), pp.540~544 (2012), 한국식품과학회[DOI]
- 이소연, "瓊玉膏의 성분 분석 및 추출물별 抗酸化 효능 비교", 대한본초학회지, 23(2), pp.123~136 (2008), 대한본초학회
- MacMicking J, "Nitric oxide and macrophage function", Ann Rev Immunol, 15, pp.323~350 (1997)
- Malacrida CR, "Yellow passion fruit seed oil (Passiflora edulis f. flavicarpa) : physical and chemical characteristics", Braz Arch Biol Techn, 55, pp.127~134 (2012)
- Moreno MIN, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina", J Ethnopharmacol, 71, pp.109~114 (2000)
- Nathan CF, "Role of nitric oxide synthesis in macrophage antimicrobial activity", Curr Opin Immunol, 3, pp.65~70 (1991)
- Oyaizu M, "Antioxidative activities of browning reaction prepared from glucosamine", Jpn J Nutr, 44, pp.307~315 (1986)
- Perina NP, "Effect of vegetal-oil emulsion and passion fruit peel-powder on sensory acceptance of functional yogurt", Food Res Int, 70, pp.134~141 (2015)
- Prior RL, "Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements", J Agric Food Chem, 53, pp.4290~4302 (2005)
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic Biol Med, 26, pp.1231~1237 (1999)
- Repetto G, "Neutral red uptake assay for the estimation of cell viability", cytotoxicity, 2008, pp.3, 1125~
- Sander CS, "Photoaging is associated with protein oxidation in human skin in vivo", J Invest Dermatol, 118, pp.618~625 (2002)
- Sunitha M, "Antioxidant activity of Passiflora edulis Sims leaves", Indian J Pharm Sci, 71, pp.310~311 (2009)
- Vámos Vigyázó L, "Polyphenol oxidases and peroxidases in fruits and vegetables", Crit Rev Food Sci Nutr, 15, pp.49~127 (1981)
- Watson RR, "Oral administration of the purple passion fruit peel extract reduces wheeze and cough and improves shortness of breath in adults with asthma", Nutr Res, 28, pp.166~171 (2008)
- Wong YS, "Influence of extraction conditions on antioxidant properties of passion fruit (Passiflora edulis) peel", Acta Sci Pol Technol Aliment, 13, pp.257~256 (2014)
- Yamaguchi Y, "Physiological factors that regulate skin pigmentation", Biofactors, 35, pp.193~199 (2009)
- Yoon S B, "Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS-activated RAW 264.7 macrophages", J Ethnopharmacol, 125, pp.286~290 (2009)