Echinacea angustifolia 열수 추출물의 항산화, 항염 효과
The Antioxidant and Anti-inflammatory Activities of Echinacea angustifolia Hot Water Extract
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목적: 본 연구에서는 Echinacea angustifolia (E. angustifolia)열수 추출물의 화장품 원료로서의 기능성을 확인하기 위해 항산화능및 항염활성을 측정하였다. 방법: 라디컬 소거능으로서 DPPH, ABTS, FRAP 실험, 항산화 물질 측정으로서 폴리페놀과 플라보노이드 농도 측정을 실시하였다. 또한, 세포실험에서는 RAW 264.7 cell을 사용하여 세포독성 실험과 항염 실험을 실시하였다. 결과: 실험결과 DPPH 실험에서는 ascorbic acid와 비교 시 27.98%의 항산화능을 나타내었으며, ABTS 실험에서는 ascorbic acid와 비교시 20.29%의 항산화능을 나타내었다. FRAP에서는E . angustifolia 추출물의 1 mg이 ascorbic acid 0.412±0.013 μg의 환원력을 보였다. 폴리페놀 농도는 104.42±3.21 mg/g으로 나타났으며, 플라보노이드 농도는 62.86±2.26 mg/g으로 나타났다. 세포실험결과 세포독성의 경우 80% 이상의 세포가 생존하여 E. angustifolia 추출물이 낮은 독성을 가지고 있음을 확인하였다. 한편 항염 활성 실험에서도 농도에 따라 염증반응을 감소시키는 동시에 200 μg/mL 농도에서 51.07±4.58%의 염증 억제능을 보였다. 결론: E. angustifolia 추출물의 코스메슈티컬 원료로서의 가능성을 확인할 수 있었다.
Abstract
Purpose: This study attempted to investigate the antioxidant and anti-inflammatory effects of Echinacea angustifolia extract. Methods: For analysis of radical scavenging activities, DPPH, ABTS and FRAP were performed. To analyze antioxidant properties, in addition, polyphenol and flavonoid concentrations were measured. In cell-based assays, cytotoxicity and anti-inflammatory assays were performed, using RAW 264.7. Results: The results found the followings: DPPH and ABTS assays revealed 27.98% and 20.29% respectively in terms of antioxidant activities when compared to ascorbic acid. In the FRAP assay, E. angustifolia extract (1 mg) showed 0.412±0.013 μg of reducing power (ascorbic acid). Polyphenol and flavonoid concentrations were 104.42±3.21 mg/g and 62.86±2.26 mg/g each. In terms of cytotoxicity, more than 80% cells survived after the assays, confirming the low toxicity of E. angustifolia extract. In anti-inflammatory activities, the extract reduced the inflammatory response and, at the same time, showed 51.07±4.58% inflammationinhibiting effects at 200 μg/mL, confirming the potential of E. angustifolia extract as an ingredient of cosmeceuticals. Conclusion: These results confirmed the potential of E. angustifolia extract as an ingredient of cosmeceuticals.
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