패션프루트 껍질의 생리활성에 대한 연구
Study on the Physiological Activity of Passiflora Edulis f. Peel
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In this study, the effects of the physiological activity of the Passiflora edulis f. peel (PEP) were verified and the applicability as a cosmetic material was reviewed. As a result of the total polyphenol content, the content was expressed as 95.88±0.45mg/100g dry in the hot water extract and 119.99±1.17mg/100g dry in the 70% ethanol extract. The antioxidant effects were measured through an experiment based on evaluating the electron-donating ability and ABTS radical scavenging activities, and the results showed that the effects were better in the 70% ethanol extract than in the hot water extract. In order to verify the whitening effect, the measurement of tyrosinase inhibitory activity showed an effect of 30.73±0.77% at 1,000 ㎍/㎖ of PEP in the 70% ethanol extract, which showed a greater whitening effect than of the hot water extract. Active suppression of nitric oxide (NO), an inflammatory inducer, was measured to verify the anti-inflammatory efficacy. Prior to that, an MTT assay was conducted in RAW 264.7 cells to assess the cell survival rate; it was found that no toxicity was apparent at any concentration. In the NO assay, experiments were conducted using a concentration of 500 ㎍/㎖ with a 100% survival rate. The NO assay was measured using Griess reagent; compared with the lipopolysaccharide treatment group and non-treatment group, it showed better activity in the ethanol extract than in the hot water extract. With the above result, it has been confirmed that PEP extract is an effective antioxidant, has whitening effects, and is an anti-inflammatory agent. In particular, the use of ethanol extracts as natural materials for cosmetics was confirmed to have excellent effects.
Abstract
In this study, the effects of the physiological activity of the Passiflora edulis f. peel (PEP) were verified and the applicability as a cosmetic material was reviewed. As a result of the total polyphenol content, the content was expressed as 95.88±0.45mg/100g dry in the hot water extract and 119.99±1.17mg/100g dry in the 70% ethanol extract. The antioxidant effects were measured through an experiment based on evaluating the electron-donating ability and ABTS radical scavenging activities, and the results showed that the effects were better in the 70% ethanol extract than in the hot water extract. In order to verify the whitening effect, the measurement of tyrosinase inhibitory activity showed an effect of 30.73±0.77% at 1,000 ㎍/㎖ of PEP in the 70% ethanol extract, which showed a greater whitening effect than of the hot water extract. Active suppression of nitric oxide (NO), an inflammatory inducer, was measured to verify the anti-inflammatory efficacy. Prior to that, an MTT assay was conducted in RAW 264.7 cells to assess the cell survival rate; it was found that no toxicity was apparent at any concentration. In the NO assay, experiments were conducted using a concentration of 500 ㎍/㎖ with a 100% survival rate. The NO assay was measured using Griess reagent; compared with the lipopolysaccharide treatment group and non-treatment group, it showed better activity in the ethanol extract than in the hot water extract. With the above result, it has been confirmed that PEP extract is an effective antioxidant, has whitening effects, and is an anti-inflammatory agent. In particular, the use of ethanol extracts as natural materials for cosmetics was confirmed to have excellent effects.
참고문헌 (28)
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