백서향 메탄올추출물의 주름개선 및 피부미백 효능
Anti-wrinkle and Hypopigmentation Efficacies of Daphne kiusiana Methanol Extract in a Cell-free System
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The current study aimed to investigate the anti-wrinkle and hypopigmentation efficacies of Daphne kiusiana methanol extract (DKME) in a cell-free system. The total polyphenol and flavonoid contents of DKME were 275.3 TAE (tannic acid equivalent) mg/g and 230.8 RE (rutin equivalent) mg/g, respectively. At concentration of 1,000 μg/mL, the electron-donating ability of DKME was 94.4% with SC50 (50% scavenging concentration for free radical) of 69.5 μg/ mL. The IC50 (50% inhibitory concentration) of collagenase activity for DKME and ascorbic acid were 631.0 μg/mL and 448.3 μg/mL, respectively. The IC50 of elastase activity for DKME and ascorbic acid were 595.1 μg/mL and over 1,000 μg/mL, respectively. The IC50 of mushroom tyrosinase activity for DKME and arbutin were 97.1 μg/mL and 175.3 μg/mL, respectively. These results indicate that DKME has high antioxidant capacity and excellent anti-wrinkle and hypopigmentation efficacies which are similar to or even superior to ascorbic acid or arbutin in a cell-free system.
Abstract
The current study aimed to investigate the anti-wrinkle and hypopigmentation efficacies of Daphne kiusiana methanol extract (DKME) in a cell-free system. The total polyphenol and flavonoid contents of DKME were 275.3 TAE (tannic acid equivalent) mg/g and 230.8 RE (rutin equivalent) mg/g, respectively. At concentration of 1,000 μg/mL, the electron-donating ability of DKME was 94.4% with SC50 (50% scavenging concentration for free radical) of 69.5 μg/ mL. The IC50 (50% inhibitory concentration) of collagenase activity for DKME and ascorbic acid were 631.0 μg/mL and 448.3 μg/mL, respectively. The IC50 of elastase activity for DKME and ascorbic acid were 595.1 μg/mL and over 1,000 μg/mL, respectively. The IC50 of mushroom tyrosinase activity for DKME and arbutin were 97.1 μg/mL and 175.3 μg/mL, respectively. These results indicate that DKME has high antioxidant capacity and excellent anti-wrinkle and hypopigmentation efficacies which are similar to or even superior to ascorbic acid or arbutin in a cell-free system.
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