Cell-free 시험법에서 가는갯능쟁이 메탄올추출물의 항 주름 및 피부미백 효능
Anti-wrinkle and Skin-whitening Efficacies of Atriplex gmelinii Methanol Extract in a Cell-free System
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
The purpose of this study was to examine the inhibitory efficacy of Atriplex gmelinii methanol extract (AGME) against wrinkle formation and hyperpigmentation in a cell-free system. The total polyphenol and flavonoid contents of AGME were 268.7 tannic acid equivalent (TAE) mg/g and 247.5 rutin equivalent (RE) mg/g, respectively. At a concentration of 1,000 μg/mL, the electron-donating ability of AGME was 83.2% with an SC50 (50% scavenging concentration for free radicals) of 249.0 μg/mL. The IC50 (50% inhibitory concentration) of collagenase activity for AGME and ascorbic acid was over 1,000 μg/mL and 448.3 μg/mL, respectively. The IC50 of elastase activity for both AGME and ascorbic acid was over 1,000 μg/mL. The IC50 of mushroom tyrosinase activity for AGME and arbutin were less than 62.5 μg/mL and 175.3 μg/mL, respectively. The results of this study demonstrate that AGME has high antioxidant capacity as well anti-wrinkle and skin-whitening efficacies in a cell-free system.
Abstract
The purpose of this study was to examine the inhibitory efficacy of Atriplex gmelinii methanol extract (AGME) against wrinkle formation and hyperpigmentation in a cell-free system. The total polyphenol and flavonoid contents of AGME were 268.7 tannic acid equivalent (TAE) mg/g and 247.5 rutin equivalent (RE) mg/g, respectively. At a concentration of 1,000 μg/mL, the electron-donating ability of AGME was 83.2% with an SC50 (50% scavenging concentration for free radicals) of 249.0 μg/mL. The IC50 (50% inhibitory concentration) of collagenase activity for AGME and ascorbic acid was over 1,000 μg/mL and 448.3 μg/mL, respectively. The IC50 of elastase activity for both AGME and ascorbic acid was over 1,000 μg/mL. The IC50 of mushroom tyrosinase activity for AGME and arbutin were less than 62.5 μg/mL and 175.3 μg/mL, respectively. The results of this study demonstrate that AGME has high antioxidant capacity as well anti-wrinkle and skin-whitening efficacies in a cell-free system.
참고문헌 (48)
- 배정진, "Atriplex gmelini(가는갯능쟁이)의 내염성과 항산화 효소 반응", Journal of Ecology and Environment, 26(5), pp.8~280 (2003), 한국생태학회
- Briganti S, "Chemical and instrumental approaches to treat hyperpigmentation", Pigment Cell Res, 16, pp.101~110 (2003)
- Cannell RJP, "Results of a large scale screen of microalgae for the production of protease inhibitors", Planta Med, 54, pp.10~14 (1998)
- Chang TS, "Natural melanogenesis inhibitors acting through the down-regulation of tyrosinase activity", Materials, 5, pp.1661~1685 (2012)
- Claus H, "Bacterial tyrosinases", Syst Appl Microbiol, 29, pp.3~14 (2006)
- Davies R, "The catalysis of the N-nitrosation of secondary amines by nitrosophenols", Food Chem, 6, pp.115~122 (1980)
- Fernando IPS, "The potential of fucoidans from Chnoospora minima and Sargassum polycystum in cosmetics : antioxidant, anti-inflammatory, skin-whitening, and antiwrinkle activities", J Appl Phycol, 30, pp.3223~ (2018)
- Folin O, "On phosphotungstic-phosphomolybdic compounds as color reagents", J Biol Chem, 12, pp.239~243 (1912)
- García-Rivera J, "Melanization of Cryptococcus neoformans reduces its susceptibility to the antimicrobial effects of silver nitrate", Med Mycol, 39, pp.353~357 (2001)
- Geng J, "Protective action of bacterial melanin against DNA damage in full UV spectrums by a sensitive plasmid-based noncellular system", J Biochem Biophys Methods, 70, pp.1151~1155 (2008)
- Hearing VJ, "Biogenesis of pigment granules: a sensitive way to regulate melanocyte function", J Dermatol Sci, 37, pp.3~14 (2005)
- Heim KE, "Flavonoid antioxidants: chemistry, metabolism, and structure-activity relationships", J Nutr Biochem, 13, pp.572~584 (2002)
- Hernández JA, "Response of antioxidant systems and leaf water relations to NaCl in pea plants", New Phytol, 141, pp.241~251 (1999)
- Hetog MGL, "Content of potentially anticarcinogenic flavonoids of tea infusions, wines and fruit juice", J Agr Food Chem, 41, pp.1242~1246 (1993)
- Howling GI, "The effect of chitin and chitosan on the proliferation of human skin fibroblasts and keratinocytes in vitro", Biomaterials, 22, pp.2959~2966 (2001)
- 정희정, "가는갯능쟁이 (Atriplex gmelinii)의 항산화 효과", KSBB Journal, 31(4), pp.200~207 (2016), 한국생물공학회[DOI]
- Jesumani V, "Unravelling property of polysaccharides from Sargassum sp. as an anti-wrinkle and skin whitening property", Int J Biol Macromol, 140, pp.216~224 (2019)
- Chanipa Jiratchayamaethasakul, "In vitro screening of elastase, collagenase, hyaluronidase, and tyrosinase inhibitory and antioxidant activities of 22 halophyte plant extracts for novel cosmeceuticals", Fisheries and Aquatic Sciences, 23(1), pp.1~9 (2020), 한국수산과학회[DOI]
- Jithesh MN, "Antioxidative response mechanisms in halophytes: their role in stress defence", J Genet, 85, pp.237~254 (2006)
- Kim JH, "Ceriporia lacerata mycelium culture medium as a novel anti-aging microbial material for cosmeceutical application", Cosmetics, 8, pp.101~ (2021)
- Kim KH, "A research trend of natural product on well-being industry", J Soc Cosmet Sci Kor, 30, pp.329~343 (2004)
- 김명현, "기후변화에 따른 국내 C_4 식물 목록과 분포 변화:고찰", 한국농림기상학회지, 13(3), pp.123~139 (2011), 한국농림기상학회
- Kong CS, "Flavonoid glycosides isolated from Salicornia herbacea inhibit matrix metalloproteinase in HT1080 cells", Toxicol In Vitro, 22, pp.1742~1748 (2008)
- 공창숙, "꽈배기모자반 추출물이 3T3-L1 지방세포 분화에 미치는 영향", Ocean and Polar Research, 37(4), pp.279~285 (2015), 한국해양과학기술원[DOI]
- Kwon BS, "Isolation and sequence of a cDNA clone for human tyrosinase that maps at the mouse c-albino locus", Proc Natl Acad Sci USA, 84, pp.7473~7477 (1987)
- Jung Im Lee, "Antioxidant Activity of the Halophyte Limonium tetragonum and Its Major Active Components", Biotechnology and Bioprocess Engineering, 16(5), pp.992~999 (2011), 한국생물공학회
- 이서현, "Antioxidant Capacity and Anti-Wrinkle Efficacy of Rhamnus davurica Methanol Extract in a Cell-free System", 대한미용학회지, 17(1), pp.1~7 (2021), 대한미용학회[DOI]
- Levy C, "MITF: master regulator of melanocyte development and melanoma oncogene", Trends Mol Med, 12, pp.406~414 (2006)
- Meot-Duros L, "Radical scavenging, antioxidant and antimicrobial activities of halophytic species", J Ethnopharmacol, 116, pp.258~262 (2008)
- Oueslati S, "Phenolic content, antioxidant, anti-inflammatory and anticancer activities of the edible halophyte Suaeda fruticosa Forsk", Food Chem, 132, pp.943~947 (2012)
- Ozgur R, "Reactive oxygen species regulation and antioxidant defence in halo phytes", Funct Plant Biol, 40, pp.832~847 (2013)
- Parfitt AM, "Procollagen type I carboxy-terminal extension peptide in serum as a marker of collagen biosynthesis in bone. Correlation with iliac bone formation rates and comparison with total alkaline phosphatase", J Bone Miner Res, 2, pp.427~436 (1987)
- Pérez GRM, "Antioxidant and free radical scavenging activities of 5, 7, 3’-trihydroxy-3, 6, 4’-trimethoxyflavone from Brickellia veronicaefolia", Phytother Res, 18, pp.428~430 (2004)
- Puupponen-Pimiä R, "Development of functional ingredients for gut health", Trends Food Sci Tech, 13, pp.3~11 (2002)
- Rio DD, "Dietary (poly) phenolics in human health: structures, bioavailability, and evidence of protective effects against chronic diseases", Antioxid Redox Signal, 18, pp.1818~1892 (2013)
- Scharffetter-Kochanek K, "Photoaging of the skin from phenotype mechanisms", Exp Gerontol, 35, pp.307~316 (2000)
- Sharma OP, "DPPH antioxidant assay revisited", Food Chem, 113, pp.1201~1205 (2009)
- Singer AJ, "Cutaneous wound healing", New Engl J Med, 2341, pp.738~746 (1999)
- Slominski A, "Melanin pigmentation in mammalian skin and its hormonal regulation", Physiol Rev Suppl, 84, pp.1155~1228 (2004)
- 손호용, "호이초(Saxifraga stolonifera) 지상부의 항균, 항혈전 및 항산화 활성 평가", 한국미생물·생명공학회지, 36(3), pp.195~200 (2008), 한국미생물·생명공학회
- Trabelsi N, "Solvent effects on phenolic contents and biological activities of the halophyte Limoniastrum monopetalum leaves", Food Sci Technol, 43, pp.632~639 (2010)
- Tsao R, "Chemistry and biochemistry of dietary polyphenols", Nutrients, 2, pp.1231~1246 (2010)
- Williams RJ, "Flavonoids: antioxidants or signaling molecules?", Free Radic Biol Med, 36, pp.838~849 (2004)
- Wlaschek M, "Solar UV irradiation and dermal photoaging", J Photochem Photobiol B Biol, 63, pp.41~51 (2001)
- Yaar M, "Photoageing: mechanism, prevention and therapy", Br J Dermatol, 157, pp.874~887 (2007)
- Yang B, "Trans-Zeatin inhibits UVB-induced matrix metalloproteinase-1 expression via MAP kinase signaling in human skin fibroblasts", Int J Mol Med, 23, pp.555~560 (2009)
- Yasui H, "Age-dependent generation of reactive oxygen species in the skin of live hairless rats exposed to UVA light", Exp Dermatol, 12, pp.655~661 (2003)
- Zhu W, "The use of botanical extracts as topical skin-lightening agents for the improvement of skin pigmentation disorders", J Investig Dermatol Symp Proc, 13, pp.20~24 (2008)