치자와 삼백초 열수추출물의 항산화능 및 콜라겐 합성능
Antioxidative and Collagen Synthetic Abilities of Gardeniae fructus and Saururus chinensis Water Extracts
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초록
The purpose of this study was to investigate the effect of Gardeniae fructus water extract (GFWE) and Saururus chinensis water extract (SCWE) on the antioxidative and collagen synthetic abilities. The contents of total polyphenol in GFWE and SCWE were 66.1 mg/g and 53.5 mg/g, respectively. The contents of total flavonoid in GFWE and SCWE were 34.5mg/g and 28.7 mg/g, respectively. Electron donating abilities of GFWE, SCWE and BHT (positive control) showed a dose response relationship and they were 24.6%, 21.3% and 82.5% at 1,000 ppm, respectively. GFWE and SCWE have potent free radical scavenging activity even though showing lower value than that of BHT. In human dermal fibroblasts, cell growth activities of GFWE and SCWE were more than 90% in all the measured concentrations. Thus, the maximum permissible levels of GFWE and SCWE in human dermal fibroblasts were confirmed to be more than 200 ppm. TGF-β_1 (5ng/mL) increased type-1 procollagen protein production in cultured human dermal fibroblasts by 127%. GFWE and SCWE increased the procollagen protein production by 34.5% and 33.9%, respectively, at the concentration of 100 ppm.
Abstract
The purpose of this study was to investigate the effect of Gardeniae fructus water extract (GFWE) and Saururus chinensis water extract (SCWE) on the antioxidative and collagen synthetic abilities. The contents of total polyphenol in GFWE and SCWE were 66.1 mg/g and 53.5 mg/g, respectively. The contents of total flavonoid in GFWE and SCWE were 34.5mg/g and 28.7 mg/g, respectively. Electron donating abilities of GFWE, SCWE and BHT (positive control) showed a dose response relationship and they were 24.6%, 21.3% and 82.5% at 1,000 ppm, respectively. GFWE and SCWE have potent free radical scavenging activity even though showing lower value than that of BHT. In human dermal fibroblasts, cell growth activities of GFWE and SCWE were more than 90% in all the measured concentrations. Thus, the maximum permissible levels of GFWE and SCWE in human dermal fibroblasts were confirmed to be more than 200 ppm. TGF-β_1 (5ng/mL) increased type-1 procollagen protein production in cultured human dermal fibroblasts by 127%. GFWE and SCWE increased the procollagen protein production by 34.5% and 33.9%, respectively, at the concentration of 100 ppm.
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