인체피부섬유아세포에서 녹차∙백차∙홍차 열수추출물의 콜라겐 합성능 비교 연구
Comparison in Collagen Synthetic Abilities of Water Extracts of Green Tea, White Tea and Black Tea in Human Dermal Fibroblasts
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This study was conducted to evaluate the collagen synthetic abilities of water extracts of green tea (GT), white tea (WT)and black tea (BT) in human dermal fibroblasts. In MTT assay, the maximum permissible level for GT, WT and BT was over 200 μg/mL, and that for EGCG was 50 μg/mL. In the experiment of type-I procollagen synthetic ability, TGF-β1(positive control) increased it by 179% (p⁄0.001) at the concentration of 5 ng/mL, compared to the control group. At the concentrations of 12.5, 25, and 50μg/mL, EGCG increased it by 49.3, 80.1 (p⁄0.01), and 141.6% (p⁄0.001), GT increased it by 8.3, 48.1, and 98.5% (p⁄0.01), WT increased it by 10.3, 38.6, 110.4% (p⁄0.05), and BT increased it by 89.5 (p⁄0.01), 96.9 (p⁄0.01), and 114.5% (p⁄0.01), respectively. The order of high to low sequence for the collagen synthetic ability was EGCG¤BT¤WT and GT. It was conformed that GT, WT and BT had high collagen synthetic abilities, and they could be a useful functional natural source for the anti-wrinkle cosmetic materials.
Abstract
This study was conducted to evaluate the collagen synthetic abilities of water extracts of green tea (GT), white tea (WT)and black tea (BT) in human dermal fibroblasts. In MTT assay, the maximum permissible level for GT, WT and BT was over 200 μg/mL, and that for EGCG was 50 μg/mL. In the experiment of type-I procollagen synthetic ability, TGF-β1(positive control) increased it by 179% (p⁄0.001) at the concentration of 5 ng/mL, compared to the control group. At the concentrations of 12.5, 25, and 50μg/mL, EGCG increased it by 49.3, 80.1 (p⁄0.01), and 141.6% (p⁄0.001), GT increased it by 8.3, 48.1, and 98.5% (p⁄0.01), WT increased it by 10.3, 38.6, 110.4% (p⁄0.05), and BT increased it by 89.5 (p⁄0.01), 96.9 (p⁄0.01), and 114.5% (p⁄0.01), respectively. The order of high to low sequence for the collagen synthetic ability was EGCG¤BT¤WT and GT. It was conformed that GT, WT and BT had high collagen synthetic abilities, and they could be a useful functional natural source for the anti-wrinkle cosmetic materials.
참고문헌 (15)
- 강성례, "치자와 삼백초 열수추출물의 항산화능 및 콜라겐 합성능", 대한미용학회지, 7(2), pp.165~171 (2011), 대한미용학회
- 구본석, "사상자(Torilis Fructus)가 섬유아세포의 Procollagen 생합성과Matrix metalloproteinase-I(MMP-1)의 활성에 미치는 영향", 생약학회지, 38(4), pp.349~353 (2007), 한국생약학회
- 김상남, "녹차 ․ 백차 ․ 홍차 열수추출물의 항산화능 비교", 대한미용학회지, 8(3), pp.165~169 (2012), 대한미용학회
- 서진영, "배양한 피부 섬유아세포에서의 Procollagenα1 (I), Tropoelastin 및 MMP-1 mRNA 발현에 미치는 RetinoicAcid 및 Vitamin C의 영향", 대한피부연구학회지, 8(1), pp.23~28 (2001)
- 한채정, "돈피 추출 콜라겐 보충 식이가 중년 여성의 혈중 콜라겐, 성호르몬, 지질대사 및 피부 갈라짐에 미치는 영향", 대한지역사회영양학회지, 13(6), pp.912~921 (2008), 대한지역사회영양학회
- Bateman JF, "Collagen superfamily extracellular matrix", pp.22~67 (1996), Harwood
- Dabbous M, "Isolation and partial characterization of bovine gingival AB collagen", Mol Cell Biochem, 34, pp.87~93 (1981)
- Lowry OH, "Protein measurement with the folin phenol reagent", J Bioll Chem, 193, pp.265~275 (1951)
- Mosmann T, "Rapid colorimetric assay for the cellular growth and survival: application to proliferation and cytotoxic assay", J Immun Methods, 65, pp.55~63 (1983)
- Parfitt AM, "Pro-collagen type I carboxy-terminal extension peptide in serum as a marker of collagen biosynthesis in bone. Correlation with iliac bone formation rates and comparison with total alkaline phosphatase", J Bone Min Res, 2, pp.427~436 (1987)
- Son ED, "Alphaketoglutarate stimulates procollagen production in cultured human dermal fibroblasts, and decreases UVB-induced wrinkle formation following topical application on the dorsal skin of hairless mice", Biol Pharm Bull, 30, pp.1395~1399 (2007)
- Talwar HS, "Reduced type I, type III procollagens in photodamaged adult human skin", J Invest Dermatol, 105, pp.285~290 (1995)
- Wulf HC, "Skin aging and natural photoprotection", Micron, 35, pp.185~191 (2004)
- Yaar M, "Photoageing: mechanism, prevention and therapy", British J Dermatol, 157, pp.874~887 (2007)
- 유정민, "양조 부산물인 주박의 주름개선 효과", 생명과학회지, 20(12), pp.1838~1843 (2010), 한국생명과학회