Antioxidant Capacity and Anti-Wrinkle Efficacy of Rhamnus davurica Methanol Extract in a Cell-free System
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The current study aimed to investigate the antioxidant capacity and anti-wrinkle efficacy of Rhamnus davurica methanol extract (RDME) in a cell-free system. The amount of total polyphenols and flavonoids in RDME were 348.5 TAE mg/g and 234.1 RE mg/g, respectively. At concentration of 1,000 μg/mL, the electron-donating ability of RDME was found to be 89.8% with an SC50 of 194.9 μg/mL. Moreover, the collagenase and elastase inhibiting activities of RDME at 1,000 μg/mL were 73.3% and 70.1%, respectively. The IC50 values of RDME for collagenase and elastase activities were calculated to be 309.0 μg/mL and 324.3 μg/mL, respectively, which were superior to those of the positive control ascorbic acid (AA) (360.3 μg/mL and over 1,000 μg/mL, respectively). As determined from MTT assay, the maximum permissible level for treating RDME to human dermal fibroblasts was 100 μg/mL.
Abstract
The current study aimed to investigate the antioxidant capacity and anti-wrinkle efficacy of Rhamnus davurica methanol extract (RDME) in a cell-free system. The amount of total polyphenols and flavonoids in RDME were 348.5 TAE mg/g and 234.1 RE mg/g, respectively. At concentration of 1,000 μg/mL, the electron-donating ability of RDME was found to be 89.8% with an SC50 of 194.9 μg/mL. Moreover, the collagenase and elastase inhibiting activities of RDME at 1,000 μg/mL were 73.3% and 70.1%, respectively. The IC50 values of RDME for collagenase and elastase activities were calculated to be 309.0 μg/mL and 324.3 μg/mL, respectively, which were superior to those of the positive control ascorbic acid (AA) (360.3 μg/mL and over 1,000 μg/mL, respectively). As determined from MTT assay, the maximum permissible level for treating RDME to human dermal fibroblasts was 100 μg/mL.
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