화장료 소재로서 토복령 추출물의 항노화 및 항산화 활성
Anti-aging and Antioxidant Activity of Smilax china Roots Extract
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This study was conducted for the purpose of examining the anti-wrinkle, whitening, astringent effect, and antioxidant activities of Smilacis rhizoma, Smilax china roots, as a cosmeceutical, viz. SRW; water extract, SRE; 80% ethanol extract, SRHW; hot water extract. The highest content of flavonoid compound was 6.19 mg·QC/g in SRE, and that for the polyphenol compound was 291.58 291.58 mg·TA/g in SRHW. In the analysis of anti-wrinkle activity at 2,000 ㎍/ mL, the highest results for collagenase and elastase inhibition were53.28% in SRE and 9.42% in SRW. The result for tyrosinase inhibition activity was 32.84~36.49% and the astringent effect was less than 9%. The highest DPPH radical scavenging ability was 96.04% in SRE. With an ABTS radical scavenging ability of 500 ㎍/mL, greater than 90% was detected for all extracts, and, in particular, 98.14% activity was detected for SHRW at 250 ㎍/mL. The highest SOD- like activity was 20.08% for SHRW at 2,000 ㎍/mL. The highest nitrite scavenging capacity for pH 1.2 was 96.21% in SRHW at 2,000 ㎍/mL, and the highest condition for pH 3.0 was 54.12% in SRE. In RAW264.7 macrophages, a decrease in SRHW of approximately 10% was observed at a concentration of 200 ㎍/mL, and SRE decreased by 13%. These results demonstrated that S. china roots can differ depending on the extraction solvent and method, and that extract from S. china roots may be useful for extraction using hot water-soluble and 80% ethanol solvent due to its exceptional anti-wrinkle, whitening, astringent effect, and DPPH and ABTS radical scavenging activity. And S. china roots is believed to be a natural resource that can be used as a cosmeceutical material.
Abstract
This study was conducted for the purpose of examining the anti-wrinkle, whitening, astringent effect, and antioxidant activities of Smilacis rhizoma, Smilax china roots, as a cosmeceutical, viz. SRW; water extract, SRE; 80% ethanol extract, SRHW; hot water extract. The highest content of flavonoid compound was 6.19 mg·QC/g in SRE, and that for the polyphenol compound was 291.58 291.58 mg·TA/g in SRHW. In the analysis of anti-wrinkle activity at 2,000 ㎍/ mL, the highest results for collagenase and elastase inhibition were53.28% in SRE and 9.42% in SRW. The result for tyrosinase inhibition activity was 32.84~36.49% and the astringent effect was less than 9%. The highest DPPH radical scavenging ability was 96.04% in SRE. With an ABTS radical scavenging ability of 500 ㎍/mL, greater than 90% was detected for all extracts, and, in particular, 98.14% activity was detected for SHRW at 250 ㎍/mL. The highest SOD- like activity was 20.08% for SHRW at 2,000 ㎍/mL. The highest nitrite scavenging capacity for pH 1.2 was 96.21% in SRHW at 2,000 ㎍/mL, and the highest condition for pH 3.0 was 54.12% in SRE. In RAW264.7 macrophages, a decrease in SRHW of approximately 10% was observed at a concentration of 200 ㎍/mL, and SRE decreased by 13%. These results demonstrated that S. china roots can differ depending on the extraction solvent and method, and that extract from S. china roots may be useful for extraction using hot water-soluble and 80% ethanol solvent due to its exceptional anti-wrinkle, whitening, astringent effect, and DPPH and ABTS radical scavenging activity. And S. china roots is believed to be a natural resource that can be used as a cosmeceutical material.
참고문헌 (63)
- An DG, "Illustrated guide to clinical medicinal herbs", pp.356~357 (2012), Hyeonamsa
- AOAC, "Official method of analysis", pp.19~ (2005), Association of official analytical chemists
- Arnao MB, "The hydrophilic and lipophilic contribution to total antioxidant activity", Food Chem, 73, pp.239~244 (2001)[DOI]
- Bartsh H, "Inhibition of endogenous nitrosation : Mechanism and implications in human cancer prevention", Mut Res, 202, pp.307~324 (1988)[DOI]
- Blois MS, "Antioxidant determination by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Cannell RJP, "Results of a large scale screen of microalgae for the production of protease inhibitors", Planta Medica, 54, pp.10~14 (1988)[DOI]
- Chen L, "Anti-hyperuricemic and nephroprotective effects of Smilax china L", J Ethnopharmacol, 135, pp.399~405 (2011)[DOI]
- Cooney RV, "N-nitrosation and N-nitration of morphpline by nitrogen dioxide in aqueous solution: Effect of vanillin and related phenols", J Agric Food Chem, 35, pp.789~793 (1987)[DOI]
- Cowan MM, "Plant products as antimicrobial agents", Clin Microbiol Rev, 12, pp.564~582 (1999)[DOI]
- Crosby NT, "N-nitrosamines : A review of chemical and biological properties and their estimation in foodstuffs", Adv Food Res, 21, pp.1~56 (1976)
- Demina NS, "Collagenolytic enzymes synthesized by microorganisms", Mikrobiologiia, 65, pp.293~304 (1996)
- DeWitt DL, "Orientation of the active site, and antigenic determinants of prostaglandin endoperoxide of synthase in the endoplasmic reticulum", J Biol Chem, 256, pp.10375~10382 (1981)
- Donnelly JK, "Superoxide dismutase in foods", A Review Food Chem, 33, pp.243~270 (1989)
- Duval B, "Phenolic compounds and antioxidant properties in the snow alga Chlamydomonas nivalis after exposure to UV light", J Appl Phycol, 11, pp.559~566 (1999)
- Fang YZ, "Free radicals, antioxidants and nutrition", Nutrition, 18, pp.872~879 (2002)
- Fisher GJ, "Pathophysiology of premature skin aging induced by ultraviolet light", N Engl J Med, 337, pp.1419~1428 (1997)
- Giacomoni PU, "Factors of skin ageing share common mechanism", Biogerontol, 2, pp.219~229 (2001)
- Greenstock CL, "Radiation and aging: free radical damage, biological response and possible antioxidant intervention", Med Hypotheses, 41, pp.473~482 (1993)
- Halliwell B, "Free radicals, antioxidants and human disease: Where are we now?", J Lab Clinical Med, 119, pp.598~620 (1992)
- Halliwell B, "Drug antioxidant effects. A basic for drug selection?", Drugs, 42, pp.569~605 (1991)
- Hanasaki Y, "The corelation between active oxygen scavenging and antioxidative effects of flavonoids", Free radic Biol Med, 16, pp.845~850 (1994)
- Hearing VJJr, "Mammalian monophenol monooxygenase(tyrosinase): purification, properties, and reactions catalyzed", Methods Enzymol, 142, pp.154~165 (1987)
- Higuchi M, "Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L–arginine-dependent mechanism acts as synergistically as the major cytolytic mechanism of activated macrophages", J Immunol, 144, pp.1425~1431 (1990)
- 장태원, "토복령의 항산화 및 산화적 DNA 손상억제 활성", Journal of Applied Biological Chemistry, 61(2), pp.109~117 (2018), 한국응용생명화학회
- 강혜숙, "청미래 덩굴 뿌리 추출물 투여가 실험동물의 체내 중금속 함량에 미치는 영향", 한국식품영양학회지, 24(2), pp.233~238 (2011), 한국식품영양학회
- Kato H, "Inhibition of nitrosamine formation by nondialyzable melanoidins", Agric Biol Chem, 51, pp.1333~1338 (1987)
- 김은영, "항산화활성을 나타내는 약용식물 소재 탐색", 한국식품과학회지, 36(2), pp.333~338 (2004), 한국식품과학회
- 김민재, "Inhibition of Cell Growth by Anoikis in Various Human Cancer Cell Lines Treated with an Extract of Smilax china L.", 생명과학회지, 31(3), pp.266~279 (2021), 한국생명과학회[DOI]
- 김인덕, "한방원료의 초임계 추출을 이용한 항노화 및 주름개선 효과", KSBB Journal, 23(6), pp.529~534 (2008), 한국생물공학회
- Kim SW, "Steroidal saponins from the rhizomes of Smilax china", J Pharmacogn, 20, pp.76~82 (1989)
- Kim YH, "Study on cosmeceutical activity of four kinds of Korea herb medicine materials", J Korean Soc Fashion Beau, 5, pp.130~138 (2007)
- "Korean Herbal Pharmacopoeia" (2022), Ministry of Food and Drug Safety
- Korner A, "Mammalian tyrosinase catalyzes three reactions in the biosynthesis of melanin", Science, 217, pp.1163~1165 (1982)
- 이진태, "함초의 생리활성기능과 화장품소재로서의 응용", 대한본초학회지, 17(2), pp.6~60 (2002), 대한본초학회
- Kang-Hee Lee, "Inhibitory effect of Smilacis Glabrae Rhizoma on nitric oxide production in the macrophage cell line RAW 264.7", Korean Journal of Acupuncture, 26(3), pp.69~76 (2009), 경락경혈학회
- 이양숙, "연 부산물 혼합 추출물의 항산화 효능", 대한미용학회지, 19(1), pp.9~19 (2023), 대한미용학회
- Li YL, "A flavonoid glycoside isolated from Smilax china L. rhizome in vitro anticancer effects on human cancer cell lines", J Ethnopharmacol, 113, pp.115~124 (2007)
- Marklund S, "Involvement of superoxide anino radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase", Eur J Biochem, 47, pp.468~474 (1974)
- 문지숙, "약용식품 추출에 대한 항산화성과 알코올 탈수소효소 저해성 연구", Food Science and Preservation, 11(2), pp.201~206 (2004), 한국식품저장유통학회
- Na MK, "Inhibitory activity of medicinal plant extracts against tyrosinase", KJKIDA, 1, pp.91~95 (2005)
- Nieva Moreno MI, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina", J Ethnopharmacol, 71, pp.109~114 (2000)
- O,Boyle AR, "A nitrite-free curing system and its application to the production of wieners", Food Technol, 50, pp.88~104 (1990)
- Park DJ, "Trend analysis and prospect of cosmetic raw materials by global cosmetic consumer market analysis", Master, Department of Chemical and Biological Engineering, pp.2010
- 박정애, "토복령 추출물의 항비만 활성", 한국미생물·생명공학회지, 42(4), pp.354~360 (2014), 한국미생물·생명공학회[DOI]
- Pellegrin NRR, "Screening of dietary carotenoid rich fruit extracts for antioxidant activities applying 2,2’-azinobis (2-ethylbenzothiazoline-6-sulfonic acid) radical cation de-colorization assay", Method Enzymol, 299, pp.379~389 (1999)
- Perlish JS, "Identification of collagen fibrils in scleroderma skin", J Invest Dermatol, 90, pp.48~54 (1998)
- Pryor WA, "Oxy-radicals and related species: their formation, lifetimes, and reactions", Annu Rev Physiol, 48, pp.657~667 (1986)
- Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic Biol Med, 26, pp.1231~1237 (1999)
- Rittié L, "UV-light-induced signal cascades and skin aging", Ageing Res Rev, 1, pp.705~720 (2002)[DOI]
- 서수정, "저근백피 추출물의 항노화 활성에 관한 연구", 대한미용학회지, 14(2), pp.179~186 (2018), 대한미용학회[DOI]
- Shao B, "Steroidal saponins from Smilax china and their anti-inflammatory activities", Phytochem, 68, pp.623~630 (2007)
- 신유진, "한약재 열수추출물의 항산화 활성 및 Xanthine Oxidase 저해 활성", 한국식품영양과학회지, 42(10), pp.1712~1716 (2013), 한국식품영양과학회
- Shu XS, "Anti-inflammatory and antinociceptive activities of Smilax china L. aqueous extract", J Ethnopharmacol, 103, pp.327~332 (2006)
- 송희순, "청미래덩굴(Smilax china) 뿌리 추출물의 항산화활성 효과", 한국식품영양과학회지, 35(9), pp.1133~1138 (2006), 한국식품영양과학회
- Surveswaran S, "Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants", Food Chem, 102, pp.938~953 (2007)
- Swan GA, "Structure, chemistry and biosynthesis of the melanins", Fortschritte Chemie Organischer Naturstoffe, 31, pp.521~582 (1974)
- Tsuji N, "The role of elastase secreted by fibroblasts in wrinkle formation: implication through selective inhibition of elastase activity", Photochem Photobiol, 74, pp.283~290 (2001)
- -bis(4-methoxy-6-nitro) benzene sulfonic acid hydrate reduction by xanthine oxidase, "Anal Biochem", 1997, 206~209
- Wu LS, "Cytotoxic polyphenols against breast tumor cell in Smilax china L", J Ethnopharmacol, 130, pp.460~464 (2010)
- Wünsch E, "Zur quantitativen bestimmung der kollagenase", Hoppe Seylers Z Physiol Chem, 333, pp.149~151 (1963)
- Yagi A, "Inhibition of mushroom-tyrosinase by aloe extract", Planta Medica, 53, pp.515~517 (1987)
- Yoshizawa S, "Antitumor promoting activity of(-)-epigallocatechin gallate, the main constitutions of tannin green tea", Phytother Res, 1, pp.44~47 (1987)
- 이종수, "각종 약용 식물로부터 노화 억제 관련 생리활성 물질의 탐색 및 추출 조건", 한국식품영양과학회지, 34(8), pp.1136~1142 (2005), 한국식품영양과학회