꼬리진달래 메탄올추출물의 항주름 및 피부미백 효능
Anti-wrinkle and Skin-whitening Efficacy of Rhododendron micranthum Methanol Extract
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본 연구는 꼬리진달래 메탄올추출물에 대하여 시약을 사용한 in vitro 방법을 통해 항산화능과 피부주름 생성 저해 및 미백 효능을 알아보았다. 꼬리진달래 메탄올추출물의 총 폴리페놀과 총 플라보노이드 함량은 각각 280.42 mg/g와 197.31 mg/g이며, 전자공여능은 꼬리진달래 메탄올추출물과 양성대조군인 ascorbic acid가 1000 μg/mL에서 각각 96.2%와 98.0%로 높은 수치를 보였다. 이를 통해 꼬리진달래 메탄올추출물은 항산화능이 높은 것을 확인하였다. 피부주름 생성 저해 지표로 사용한 collagenase 활성저해능은 꼬리진달래 메탄올추출물과 양성대조군인 ascorbic acid가 1000 μg/mL에서 각각 74.5%와 79.3%, IC50은 각각 128.6 μg/mL와 123.8 μg/mL로 대등한 수치를 보였다. Elastase 활성저해능은 1000 μg/mL에서 각각 72.0%와 40.6%, IC50은 각각 232.1 μg/mL와 1000 μg/mL 이상으로 꼬리진달래 메탄올추출물이 ascorbic acid보다 elastase 저해능이 우수하였다. 피부 미백 지표로 사용한 tyrosinase 활성저해능은 꼬리진달래 메탄올추출물과 양성대조군인 arbutin이 1000 μg/mL에서 각각 69.0%와 66.0%, IC50은 각각 420.0 Anti-wrinkle and Skin-whitening Efficacy of Rhododendron micranthum 410 μg/mL, 474.9 μg/mL로 꼬리진달래 메탄올추출물이 arbutin보다 tyrosinase 활성저해능이 우수하였다. 이들 결과를 종합해 볼 때, 꼬리진달래 메탄올추출물은 주름개선 및 피부미백용 기능성화장품 천연소재로 활용가능성이 높을 것으로 생각된다.
Abstract
The purpose of this study was to investigate the anti-wrinkle and skin- whitening efficacy of Rhododendron micranthum methanol extract (RMME) in a cell-free system. The total polyphenol and flavonoid contents of RMME were 280.42 mg/g and 197.31 mg/g, respectively. The electron-donating abilities of RMME and ascorbic acid were 96.2% and 98.0% at 1,000 μg/mL, respectively. The IC50 (50% inhibitory concentration) of collagenase activity for RMME and ascorbic acid were 128.6 μg/mL and 123.8 μg/mL, respectively. The IC50 of elastase activity for RMME and ascorbic acid were 232.1 μg/mL and greater than 1,000 μg/mL, respectively. The IC50 of mushroom tyrosinase activity for RMME and arbutin were 420.0 μg/mL and 474.9 μg/mL, respectively. These results indicate that RMME has high antioxidant capacity and excellent anti-wrinkle and skin whitening effects, which are similar to or even superior to ascorbic acid or arbutin in a cell-free system.
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