유산소운동에 따른 혈관 내피세포의 SIRT1 발현이 혈압개선에 미치는 효과
Aerobic Exercise Ameliorates Blood Pressure by the Enhanced SIRT1 Expression in Vascular Endothelial Cells
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본연구는 유산소운동에 따른 혈압의 개선에 있어 혈관 내피세포에서의 산화스트레스 저항성 증진의 생리학적 역할 및분자생물학적 기전을 밝히는 데 목적이 있다. 14명의 고혈압 전단계 중년여성에게 1일 1시간, 주 2-3회 10주간의 유산소운동을실시한 결과, 체지방률, 이완기 혈압 및 안정시 심박수에서 유의한 감소가 나타났다. H2O2에 노출하여 세포노화를 촉진한HUVEC (Human Umbilical Vein Endothelial Cells)에서 활성산소의 유의한 생성 증가 및 항산화효소 발현의 감소가 나타났으나,전단응력을 사전 처치한 세포군에서는 활성산소의 증가가 억제되었다.그러나 이러한 전단응력 효과는 SIRT1 발현을 억제하였을 때 사라졌으며, SIRT1의 역할을 재검증하기 위해 SIRT1 활성제인 resveratrol을 사전 처치한 세포군에서도 H2O2 노출에 따른 활성산소의 증가가 억제됨을 알수있었다.본연구에서는 유산소운동을 통한 혈관내피세포의 SIRT1 발현이 혈관조직 내활성산소 증가를 억제함으로써 혈압의 개선에 긍정적인 역할을 할수있음을 검증하였다.
Abstract
The purpose of this study is to elucidate the role of aerobic exercise and its underlying mechanism on the improvement of blood pressure. Aerobic exercise for 1 hour per day, 2 to 3 times a week for 10 weeks decreased %body fat, diastolic blood pressure, and resting heart rate in female pre-hypertensives. A significant increase of ROS production and a decrease of catalase expression were found in H2O2 treatment-induced senescent HUVECs. Interestingly, the increase of ROS production was suppressed in the cell group pre-treated with shear stress. However, the effect of shear stress was disappeared when SIRT1 expression was inhibited. It was also found that the increase of ROS production following H2O2 exposure was suppressed even in the cell group pre-treated with resveratrol, a SIRT1 activator. In this study, it was verified that the aerobic exercise-induced enhanced SIRT1 expression can play a positive role in the improvement of blood pressure partly by suppressing ROS production in vascular endothelial cells.
참고문헌 (20)
- Agarwal, D., "Role of proinflammatory cytokines and redox homeostasis in exercise-induced delayed progression of hypertension in spontaneously hypertensive rats", Hypertension, 54, pp.1393~1400 (2009)
- Amaral, S. L., "Exercise training normalizes wall-tolumen ratio of the gracilis muscle arterioles and reduces pressure in spontaneously hypertensive rats", J Hypertension, 18, pp.1563~1572 (2000)
- Collier, S. R., "Effect of 4 weeks of aerobic or resistance exercise training on arterial stiffness, blood flow and blood pressure in pre-and stage-1hypertensives", J Hum Hypertension, 22, pp.678~686 (2008)
- Drummond, G. R., "Combating oxidative stress in vascular disease : NADPH oxidases as therapeutic targets", Nat Rev Drug Discov, 10, pp.453~471 (2011)
- Fagard, R. H., "Effect of exercise on blood pressure control in hypertensive patients", Eur J Cardiovasc Prev. Rehabil, 14, pp.12~17 (2007)
- Gomez-Cabrera, M. C., "Moderate exercise is an antioxidant : Upregulation of antioxidant genes by training", Free Radic Biol Med, 44, pp.126~131 (2008)
- GuiMaraes, G. V., "Effects of continuous vs. interval exercise training on blood pressure and arterial stiffness in treated hypertension", Hypertension Res, 33, pp.627~632 (2010)
- Horta, P. P., "Exercise training attenuates blood pressure elevation and adverse remodeling in the aorta of spontaneously hypertensive rats", Life Sci, 77, pp.3336~3343 (2005)
- Kimura, H., "Effect of endurance exercise training on oxidative stress in spontaneously hypertensive rats(SHR)after emergence of hypertension", Clin Exp Hypertension, 32, pp.407~415 (2010)
- Lee, M. Y., "Redox signaling, vascular function and hypertension", Antioxid Redox Signal, 10, pp.1045~1059 (2008)
- Li, Z., "Oxygen-derived free radicals contribute to baroreceptor dysfunction in atherosclerotic rabbits", Circ Res, 79, pp.802~811 (1996)
- Martin, J. E., "Controlled trial of aerobic exercise in hypertension", Circulation, 81(5), pp.1560~1567 (1990)
- Melo, R. M., "Training-induced pressure-lowering effect in SHR: Wide effects on circulatory profile of exercised and nonexercised muscles", Hypertension, 42, pp.851~857 (2003)
- Mitchell, J. A., "The impact of combined health factors on cardiovascular disease mortality", Am Heart J, 160, pp.102~108 (2010)
- Roque, F. R., "Aerobic exercise reduces oxidative stress and improves vascular changes of small mesenteric and coronary arteries in hypertension", Brit J Pharmacol, 168, pp.686~703 (2013)
- Rowell, L. B, "Human cardiovascular control" (1993), Oxford University Press
- Rush, J. W., "Exercise training regulates SOD-1 and oxidative stress in porcine aortic endothelium", Am J Physiol Heart Circ Physiol, 284, pp.1378~1387 (2003)
- Touyz, R. M., "Reactive oxygen species, vascular oxidatve stress, and redox signaling in hypertension: what is the clinical significance?", Hypertension, 44, pp.248~252 (2004)
- Weinsier, R. L., "The relative contribution of body fat and fat pattern to blood pressure level", Hypertension, 7(4), pp.578~585 (1985)
- Yang, D., "Protective effects of quercetin and taraxasterol against H2O2‑induced human umbilical vein endothelial cell injury in vitro", Experimental and Therapeutic Medicine, 10(4), pp.1253~1260 (2015)