유산소성 운동이 혈관 내피세포의 오토파지에 미치는 효과
Effects of Aerobic Exercise on the Autophagy-Related Protein Expressions in Vascular Endothelial Cells
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PURPOSE: Autophagy is a highly conserved process that maintains cellular energy and redox homeostasis by degrading damaged cytosolic constituents in response to external stress. The purpose of this study was to determine the effect of exercise-induced laminar shear stress (LSS) on the autophagy-related protein expressions in vascular endothelial cells. METHODS: The orbital-shaker LSS was applied to the cultured human aortic endothelial cells (HAECs) or human umbilical vein endothelial cells (HUVECs) in the intensity of 20 dyne/cm2 for 24 hours. LSS was estimated as: τmax=α√ (p×η (2πf)3). Ten C57BL/6 male mice were subjected to the voluntary wheel running for 12 weeks, while the other ten mice were used as sedentary control. The protein expressions of autophagy-related genes were determined by western-blotting. RESULTS: In HAECs, Atg3 expression was significantly increased by LSS, while Atg7 and LC3II only showed increased patterns. In HUVECs, expressions of Atg3 and LC3II were significantly increased, while p62 was significantly decreased. In exercised mice, Atg3, Atg7 and LC3II expressions were significantly increased. CONCLUSIONS: The current study has shown that the exercise-induced increased LSS enhances the level of autophagy-related protein expressions in vascular endothelial cells.
Abstract
PURPOSE: Autophagy is a highly conserved process that maintains cellular energy and redox homeostasis by degrading damaged cytosolic constituents in response to external stress. The purpose of this study was to determine the effect of exercise-induced laminar shear stress (LSS) on the autophagy-related protein expressions in vascular endothelial cells. METHODS: The orbital-shaker LSS was applied to the cultured human aortic endothelial cells (HAECs) or human umbilical vein endothelial cells (HUVECs) in the intensity of 20 dyne/cm2 for 24 hours. LSS was estimated as: τmax=α√ (p×η (2πf)3). Ten C57BL/6 male mice were subjected to the voluntary wheel running for 12 weeks, while the other ten mice were used as sedentary control. The protein expressions of autophagy-related genes were determined by western-blotting. RESULTS: In HAECs, Atg3 expression was significantly increased by LSS, while Atg7 and LC3II only showed increased patterns. In HUVECs, expressions of Atg3 and LC3II were significantly increased, while p62 was significantly decreased. In exercised mice, Atg3, Atg7 and LC3II expressions were significantly increased. CONCLUSIONS: The current study has shown that the exercise-induced increased LSS enhances the level of autophagy-related protein expressions in vascular endothelial cells.
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