전단응력이 혈관내피세포의 마이크로파티클 생성에 미치는 영향
Effects of laminar Shear Stress on Hydrogen Peroxide-Induced Microparticle Production in Human Endothelial Cells
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초록
본 연구의 목적은 혈류흐름으로 인해 혈관에 부여되는 자극(전단응력)을 in vitro에 구현하여 혈관내피세포 (Endothelium)의 마이크로파티클(Microparticle) 생성에 미치는 영향을 확인하고 이와 관련한 생물학적 기전 을 규명하는 것이다. 인간제대정맥내피세포(Human Umbilical Vein Endothelial Cell, HUVEC)를 배양 후 전 단응력을 처치한 결과, 전단응력이 HUVEC에서 분비하는 마이크로파티클과 미토콘드리아 활성산소종 생성 을 유의하게 감소시켰고, 미토콘드리아 생합성 인자(PGC-1α)는 유의하게 증가시켰음을 확인하였다. 또한, SIRT1 inhibitor(sirtinol) 처치 시 전단응력의 긍정적인 효과가 소멸함을 확인하였다. 해당 결과는 전단응력이 HUVEC에서 마이크로파티클을 SIRT1 의존적으로 조절함을 보여주었다. 본 연구는 규칙적인 운동을 통해 유도되는 전단응력이 혈관건강을 향상시킬 수 있음을 분자적/세포학적으로 보여주었다.
Abstract
The purpose of this study was to identify the effects of laminar shear stress on microparticle production and its possible mechanisms in human-derived endothelial cells. The laminar shear stress was directly associated with decreased production of microparticle and mitochondrial reactive oxygen species. The protein expressions of a key mitochondrial biogenesis marker, PGC-1α was significantly increased after the treatment of laminar shear stress. The positive effects of laminar shear stress was abolished by the treatment of sirtinol, indicating that the positive effects of shear stress is SIRT1-dependent in endothelial cells. The results of this study are cellular/molecular verification that regular exercise can improve vascular health.
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