Osteoblastic cell behavior to tantalum coating on Ti-6Al-4V alloy
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Tantalum (Ta) coating is expected to improve clinical outcomes of titanium (Ti) dental implants due to its anticorrosion and biocompatibility. The purpose of this study was to evaluate the effect of Ta coating on surface characteristics and osteoblastic cell response of Ti-6Al-4V alloy surface. Radio frequency (RF) magnetron sputtering method was used to develop the Ta coating layer on Ti- 6Al-4V disks. Surface properties were analyzed by scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). Electrochemical test was performed to evaluate the corrosion behavior. Cell responses were evaluated using 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) assay and alkaline phosphatase (ALP) activity. Ta coating layer was formed with a uniform thickness. In electrochemical test, the passive layer was more uniform and stable in Ta-coating group than Ti-6Al-4V group. Cell morphology showed no difference between Ti-6Al-4V group and Ta-coating group. Ta-coating group showed higher cell proliferation than Ti-6Al-4V group at 1, 3 and 5 days (p<0.05). There was no significant difference in ALP activity level between two groups (p>0.05). These results suggest that Ta coating on Ti-6Al-4V alloy could enhance surface properties and early osteogenic responses.
Abstract
Tantalum (Ta) coating is expected to improve clinical outcomes of titanium (Ti) dental implants due to its anticorrosion and biocompatibility. The purpose of this study was to evaluate the effect of Ta coating on surface characteristics and osteoblastic cell response of Ti-6Al-4V alloy surface. Radio frequency (RF) magnetron sputtering method was used to develop the Ta coating layer on Ti- 6Al-4V disks. Surface properties were analyzed by scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). Electrochemical test was performed to evaluate the corrosion behavior. Cell responses were evaluated using 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT) assay and alkaline phosphatase (ALP) activity. Ta coating layer was formed with a uniform thickness. In electrochemical test, the passive layer was more uniform and stable in Ta-coating group than Ti-6Al-4V group. Cell morphology showed no difference between Ti-6Al-4V group and Ta-coating group. Ta-coating group showed higher cell proliferation than Ti-6Al-4V group at 1, 3 and 5 days (p<0.05). There was no significant difference in ALP activity level between two groups (p>0.05). These results suggest that Ta coating on Ti-6Al-4V alloy could enhance surface properties and early osteogenic responses.
참고문헌 (23)
- Dehua Li, "Biomechanical comparison of the sandblasted and acid-etched and the machined and acid-etched titanium surface for dental implants", Journal of Biomedical Materials Research, 60(2), pp.325~332 (2002), Wiley-Blackwell[DOI]
- L. Le Guéhennec, "Surface treatments of titanium dental implants for rapid osseointegration", Dental Materials, 23(7), pp.844~854 (2007), Elsevier BV[DOI]
- Rüdiger Junker, "Effects of implant surface coatings and composition on bone integration: a systematic review", Clinical Oral Implants Research, 20, pp.185~206 (2009), Wiley-Blackwell[DOI]
- Gustavo Mendonça, "Advancing dental implant surface technology – From micron- to nanotopography", Biomaterials, 29(28), pp.3822~3835 (2008), Elsevier BV[DOI]
- Vincent Offermanns, "Enhanced osseointegration of endosseous implants by predictable sustained release properties of strontium", Journal of Biomedical Materials Research Part B: Applied Biomaterials, 103(5), pp.1099~1106 (2015), Wiley-Blackwell[DOI]
- Giuseppe Maria de Peppo, "Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro", International Journal of Nanomedicine, pp.2499~ (2014), Dove Medical Press Ltd.[DOI]
- Lise T. de Jonge, "Organic–Inorganic Surface Modifications for Titanium Implant Surfaces", Pharmaceutical Research, 25(10), pp.2357~2369 (2008), Springer Nature[DOI]
- Y YANG, "A review on calcium phosphate coatings produced using a sputtering process?an alternative to plasma spraying", Biomaterials, 26(3), pp.327~337 (2005), Elsevier BV[DOI]
- 박기덕, "Effect of magnesium and calcium phosphate coatings on osteoblastic responses to the titanium surface", The Journal of Advanced of Prosthodontics, 5(4), pp.402~408 (2013), 대한치과보철학회[DOI]
- María-Dolores Bermúdez, "Erosion–corrosion of stainless steels, titanium, tantalum and zirconium", Wear, 258(1-4), pp.693~700 (2005), Elsevier BV[DOI]
- Kato H, "Bonding of alkaliand heat-treated tantalum implants to bone", J Biomed Mater Res, 53, pp.28~35 (2000)
- Miyaza T, "Mechanism of bonelike apatite formation on bioactive tantalum metal in a simulated body fluid", Biomaterials, 23, pp.827~832 (2002)
- Vamsi Krishna Balla, "Direct laser processing of a tantalum coating on titanium for bone replacement structures", Acta Biomaterialia, 6(6), pp.2329~2334 (2010), Elsevier BV[DOI]
- Sompop Bencharit, "Immediate placement of a porous-tantalum, trabecular metal-enhanced titanium dental implant with demineralized bone matrix into a socket with deficient buccal bone: A clinical report", The Journal of Prosthetic Dentistry, 113(4), pp.262~269 (2015), Elsevier BV[DOI]
- Van Oss CJ, "Monopolar surfaces", Adv Colloid Interface Sci, 28, pp.35~64 (1987)
- C.F. Almeida Alves, "Bioactivity response of Ta1-xOx coatings deposited by reactive DC magnetron sputtering", Materials Science and Engineering: C, 58, pp.110~118 (2016), Elsevier BV[DOI]
- C. Arnould, "Titanium modified with layer-by-layer sol–gel tantalum oxide and an organodiphosphonic acid: A coating for hydroxyapatite growth", Journal of Colloid and Interface Science, 336(2), pp.497~503 (2009), Elsevier BV[DOI]
- Yoshimitsu Okazaki, "Comparison of metal release from various metallic biomaterials in vitro", Biomaterials, 26(1), pp.11~21 (2005), Elsevier BV[DOI]
- Lucie Bacakova, "Modulation of cell adhesion, proliferation and differentiation on materials designed for body implants", Biotechnology Advances, 29(6), pp.739~767 (2011), Elsevier BV[DOI]
- V. PeŠŠková, "The influence of implant surface properties on cell adhesion and proliferation", Journal of Materials Science: Materials in Medicine, 18(3), pp.465~473 (2007), Springer Nature[DOI]
- Louis C. Gerstenfeld, "Expression of differentiated function by mineralizing cultures of chicken osteoblasts", Developmental Biology, 122(1), pp.49~60 (1987), Elsevier BV[DOI]
- T. Berglundh, "Spontaneous progression of ligature induced peri-implantitis at implants with different surface roughness: an experimental study in dogs", Clinical Oral Implants Research, 18(5), pp.655~661 (2007), Wiley-Blackwell[DOI]
- Albrektsson T, "Direct bone anchorage of oral implants : clinical and experimental considerations of the concept of osseointegration", Int J Prosthodont, 3, pp.30~41 (1990)