Surface characteristics and osteoblastic cell response of alkali-and heat-treated titanium- 8tantalum-3niobium alloy
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Purpose: The aim of the present study was to evaluate the biological response of alkali- and heat-treated titanium-8tantalum-3niobium surfaces by cell proliferation and alkaline phosphatase (ALP) activity analysis. Methods: Commercial pure titanium (group cp-Ti) and alkali- and heat-treated titanium-8tantalum-3niobium (group AHT)disks were prepared. The surface properties were evaluated using scanning electron microscopy, energy dispersed spectroscopy and X-ray photoelectron spectroscopy (XPS). The surface roughness was evaluated by atomic force microscopy and a profilometer. The contact angle and surface energy were also analyzed. The biological response of fetal rat calvarial cells on group AHT was assessed by cell proliferation and ALP activity. Results: Group AHT showed a flake-like morphology microprofile and dense structure. XPS analysis of group AHT showed an increased amount of oxygen in the basic hydroxyl residue of titanium hydroxide groups compared with group cp-Ti. The surface roughness (Ra) measured by a profilometer showed no significant difference (P>0.05). Group AHT showed a lower contact angle and higher surface energy than group cp-Ti. Cell proliferation on group AHT surfaces was significantly higher than on group cp-Ti surfaces (P<0.05). In comparison to group cp-Ti, group AHT enhanced ALP activity (P<0.05). Conclusions: These results suggest that group AHT stimulates osteoblast differentiation.
Abstract
Purpose: The aim of the present study was to evaluate the biological response of alkali- and heat-treated titanium-8tantalum-3niobium surfaces by cell proliferation and alkaline phosphatase (ALP) activity analysis. Methods: Commercial pure titanium (group cp-Ti) and alkali- and heat-treated titanium-8tantalum-3niobium (group AHT)disks were prepared. The surface properties were evaluated using scanning electron microscopy, energy dispersed spectroscopy and X-ray photoelectron spectroscopy (XPS). The surface roughness was evaluated by atomic force microscopy and a profilometer. The contact angle and surface energy were also analyzed. The biological response of fetal rat calvarial cells on group AHT was assessed by cell proliferation and ALP activity. Results: Group AHT showed a flake-like morphology microprofile and dense structure. XPS analysis of group AHT showed an increased amount of oxygen in the basic hydroxyl residue of titanium hydroxide groups compared with group cp-Ti. The surface roughness (Ra) measured by a profilometer showed no significant difference (P>0.05). Group AHT showed a lower contact angle and higher surface energy than group cp-Ti. Cell proliferation on group AHT surfaces was significantly higher than on group cp-Ti surfaces (P<0.05). In comparison to group cp-Ti, group AHT enhanced ALP activity (P<0.05). Conclusions: These results suggest that group AHT stimulates osteoblast differentiation.
참고문헌 (28)
- Park JB, "Biomaterials: an introduction. 2nd ed" (1992), Kluwer Academic Publishers
- Cochran DL, "Bone response to unloaded and loaded titanium implants with a sandblasted and acid-etched surface: a histometric study in the canine mandible", J Biomed Mater Res, 40, pp.1~11 (1998)
- Li D, "Biomechanical comparison of the sandblasted and acid-etched and the machined and acid-etched titanium surface for dental implants", J Biomed Mater Res, 60, pp.325~332 (2002)
- Kokubo T, "Spontaneous formation of bonelike apatite layer on chemically treated titanium metals", J Am Ceram Soc, 79, pp.1127~1129 (1996)
- Shi S, "The role of type I collagen in the regulation of the osteoblast phenotype", J Bone Miner Res, 11, pp.1139~1145 (1996)
- Sisk MA, "Inhibition of cyclooxygenase by indomethacin modulates osteoblast response to titanium surface roughness in a time-dependent manner", Clin Oral Implants Res, 12, pp.52~61 (2001)
- de Groot K, "Calcium phosphate coatings for medical implants", Proc Inst Mech Eng H, 212, pp.137~147 (1998)
- Nishiguchi S, "The effect of heat treatment on bone-bonding ability of alkali-treated titanium", Biomaterials, 20, pp.491~500 (1999)
- Nishiguchi S, "Alkali- and heat-treated porous titanium for orthopedic implants", J Biomed Mater Res, 54, pp.198~208 (2001)
- Nishiguchi S, "Biology of alkali- and heat-treated titanium implants", J Biomed Mater Res A, 67, pp.26~35 (2003)
- Green J, "Cell-matrix interaction in bone: type I collagen modulates signal transduction in osteoblast-like cells", Am J Physiol, 268(5), pp.C1090~C1103 (1995)
- McCarthy TL, "Further biochemical and molecular characterization of primary rat parietal bone cell cultures", J Bone Miner Res, 3, pp.401~408 (1988)
- Healy KE, "Hydration and preferential molecular adsorption on titanium in vitro", Biomaterials, 13, pp.553~561 (1992)
- Luben RA, "Biochemical characterization with parathormone and calcitonin of isolated bone cells: provisional identification of osteoclasts and osteoblasts", Endocrinology, 99, pp.526~534 (1976)
- Lincks J, "Response of MG63 osteoblast-like cells to titanium and titanium alloy is dependent on surface roughness and composition", Biomaterials, 19, pp.2219~2232 (1998)
- Kawahara H, "In vitro study on bone formation and surface topography from the standpoint of biomechanics", J Mater Sci Mater Med, 15, pp.1297~1307 (2004)
- Berglundh T, "Spontaneous progression of ligature induced peri-implantitis at implants with different surface roughness: an experimental study in dogs", Clin Oral Implants Res, 18, pp.655~661 (2007)
- Abrahamsson I, "Early bone formation adjacent to rough and turned endosseous implant surfaces. An experimental study in the dog", Clin Oral Implants Res, 15, pp.381~392 (2004)
- Pesskova V, "The influence of implant surface properties on cell adhesion and proliferation", J Mater Sci Mater Med, 18, pp.465~473 (2007)
- Sultana R, "Surface modification of titanium with hydrothermal treatment at high pressure", Dent Mater J, 25, pp.470~479 (2006)
- Jonasova L, "Biomimetic apatite formation on chemically treated titanium", Biomaterials, 25, pp.1187~1194 (2004)
- Kim HM, "Graded surface structure of bioactive titanium prepared by chemical treatment", J Biomed Mater Res, 45, pp.100~107 (1999)
- Kim HM, "Effect of heat treatment on apatite-forming ability of Ti metal induced by alkali treatment", J Mater Sci Mater Med, 8, pp.341~347 (1997)
- Tamilselvi S, "In vitro and in vivo studies of alkali- and heat-treated Ti-6Al-7Nb and Ti-5Al-2Nb-1Ta alloys for orthopedic implants", J Biomed Mater Res A, 90, pp.380~386 (2009)
- Zhao G, "High surface energy enhances cell response to titanium substrate microstructure", J Biomed Mater Res A, 74, pp.49~58 (2005)
- Lian JB, "The developmental stages of osteoblast growth and differentiation exhibit selective responses of genes to growth factors (TGF beta 1) and hormones (vitamin D and glucocorticoids)", J Oral Implantol, 19, pp.95~105 (1993)
- Genge BR, "Correlation between loss of alkaline phosphatase activity and accumulation of calcium during matrix vesicle-mediated mineralization", J Biol Chem, 263, pp.18513~18519 (1988)
- Chosa N, "Characterization of apatite formed on alkaline-heat-treated Ti", J Dent Res, 83, pp.465~469 (2004)