Tenebrio molitor larval hot water extract inhibits flagellin-induced inflammation in Caco-2 cells
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Tenebrio molitor belongs to the order Coleoptera and family Tenebrionidae. In this study, we investigated the anti-inflammatory effects of the Tenebrio molitor larval hot water extract (TMH) on flagellin-stimulated Caco-2 human colon carcinoma cells. Flagellin induced the production of interleukin-8 in intestinal epithelial cells; however, TMH significantly suppressed this effect. Additionally, TMH effectively reduced the expression of inflammation-related genes, including interleukin-8, C-C motif chemokine ligand (CCL)-2, CCL5, CCL20, and C-X-C motif chemokine ligand 10, which was increased by flagellin. TMH exerted its anti-inflammatory effects by inhibiting the phosphorylation of key signaling molecules in the mitogen-activated protein kinase pathway, including p38, c-Jun N-terminal kinase, and extracellular signal regulated kinase, in flagellin-treated Caco-2 cells. Overall, this study demonstrates the anti inflammatory effects of TMH on flagellin-stimulated Caco-2 cells, suggesting that TMH is a promising candidate for novel therapeutic strategies to treat inflammatory bowel disease and intestinal inflammation.
Abstract
Tenebrio molitor belongs to the order Coleoptera and family Tenebrionidae. In this study, we investigated the anti-inflammatory effects of the Tenebrio molitor larval hot water extract (TMH) on flagellin-stimulated Caco-2 human colon carcinoma cells. Flagellin induced the production of interleukin-8 in intestinal epithelial cells; however, TMH significantly suppressed this effect. Additionally, TMH effectively reduced the expression of inflammation-related genes, including interleukin-8, C-C motif chemokine ligand (CCL)-2, CCL5, CCL20, and C-X-C motif chemokine ligand 10, which was increased by flagellin. TMH exerted its anti-inflammatory effects by inhibiting the phosphorylation of key signaling molecules in the mitogen-activated protein kinase pathway, including p38, c-Jun N-terminal kinase, and extracellular signal regulated kinase, in flagellin-treated Caco-2 cells. Overall, this study demonstrates the anti inflammatory effects of TMH on flagellin-stimulated Caco-2 cells, suggesting that TMH is a promising candidate for novel therapeutic strategies to treat inflammatory bowel disease and intestinal inflammation.
참고문헌 (20)
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